LOCUS Evcara 16285 bp DNA linear 16-JUL-2024 DEFINITION Microbacterium phage Evcara. ACCESSION VERSION KEYWORDS . SOURCE Microbacterium phage Evcara ORGANISM Microbacterium phage Evcara Viruses; dsDNA viruses, no RNA stage; Caudovirales; Siphoviridae. REFERENCE 1 (bases 1 to 16285) AUTHORS Bland,J.A., Bonogafsky,H.B., Brown,K.D., DeRosa,E.D., Franke,E.L., Freer,K.M., Golden,E.A., Grover,C.M., Kennedy,S.M., Lady,F.L., Martin,A.M., Mens,J.M., Parker,C.J., Perkins,A.P., Price,A.M., Pyle,A.J., Rowley,G., Sanderson,T.B., Trivette,O.S., Vaughan,E., Walker,M.W., Wilcox,J.R., Williams,V.P., Yost,K.A., Gainey,M.D., Viland,M.D., Lewis,C.M., Garlena,R.A., Russell,D.A., Jacobs-Sera,D. and Hatfull,G.F. TITLE Direct Submission JOURNAL Submitted (16-JUL-2024) Chemistry and Physics, Western Carolina University, 460 Memorial Drive, Cullowhee, NC 28723, USA COMMENT Phage isolation, DNA preparation, and annotation analysis was performed at Western Carolina University, Cullowhee, NC Sequencing by Illumina to approximately 5008x coverage was performed at the Pittsburgh Bacteriophage Institute, Pittsburgh, PA Assembly performed with Newbler and Consed software as of Jan, 2024. Supported by the Science Education Alliance, Howard Hughes Medical Institute, Chevy Chase, MD This genome has covalent terminal protein bound, defined ends. FEATURES Location/Qualifiers source 1..16285 /organism="Microbacterium phage Evcara" /mol_type="genomic DNA" /isolation_source="compost, Cullowhee, NC" /lab_host="Microbacterium paraoxydans NRRL B-24275" /country="USA" /lat_lon="35.317780 N 83.180832 W" /collection_date="01-Sep-2023" /collected_by="Ginavieve Rowley" /identified_by="Ginavieve Rowley" /note="complete genome" gene 485..967 /gene="1" /locus_tag="SEA_EVCARA_1" CDS 485..967 /gene="1" /locus_tag="SEA_EVCARA_1" /codon_start=1 /transl_table=11 /product="SSB protein" /translation="MYFRGRHTFNHRLTKGNKMSNEVAVIEQTPGSNLRPFTSVEGSD METRFSLAAAVMGATPLADHKGVPFNLRHIVRQPVEVVNQQTKAKSITYRTTLITDDG RAFSATSRGIDVAVDNILATLGNPKEWGDSSFPFIAEERGSGTSKFLTLVPVVAKGKK " gene 1028..1795 /gene="2" /locus_tag="SEA_EVCARA_2" CDS 1028..1795 /gene="2" /locus_tag="SEA_EVCARA_2" /codon_start=1 /transl_table=11 /product="DNA terminal protein" /translation="MLPGIVALRAAALKKHANAVSKVSRLKARGVDVAGTEFDPRRPW ATVKRYNSKQLESYMERLDRFNARTFRLNALADRKPLPLGRWEQYQRTERQYNRKVDS ALDRIGDLVPPGSGLSLRQREDMRKSVNGTANTENTILHTTNRKPQYVKDMNALEKLE AQLVRRMSDEYQIGQIQSSRVSAEKMVDAIGAQDINDALAELSDEQFDILWSRTRFRD TLIAVYVEDEDSKDNTHVGYDLDDARQLISWAKGLRL" gene 1746..3494 /gene="3" /locus_tag="SEA_EVCARA_3" CDS 1746..3494 /gene="3" /locus_tag="SEA_EVCARA_3" /codon_start=1 /transl_table=11 /product="DNA polymerase" /translation="MTTLDSSSVGRKVSASKKGKEWLAADFETTTDPFDVRVWAWGVA RVSNPNDVVIDNSIESFIDFISMDDSITFFHNLRFDGLFILDHILNQGFTYVEGRANP KPKEFKALISDKNKFYSITVCWENGVITEFRDSAKKFPGMSISRIAKTFGLEESKGTI DYHAPRPVGHQITPEEREYLNDDVVILAKALQITKETGLVKLTVASDAKAEYLSLVGD KLYEARFPVLSYAMDAELRRAYRGGFTYADPRFLQQRTGSGIVLDVNSLYPYIMSDRP LPYGEPEYVPGEVETTPERPLAVFSITFTARLKADHIPCIQIKGTSMFAPTEYLSVID EPTTLMMTNIDFQLYQDHYDIEILEYHGGWRFKAARGMFDVYIQKWAKIKAESTGGLR ELAKLQLNSLYGKFGANPNVRGKIPTLTDGKVKLELGAPDVRDSMYVPIAVFVTSYGR DLTIRAGQENYDTFAYADTDSLHLLRDTVPDNIEVDDKRMGAWALEYAFDQAFYVRAK VYLEHIPGIDPDCDDPDCTARHDYHNAFAGVPDRISNRLTFDDAWNGNIFTGKMIPQI VPGGVVLKDVPYELKF" gene 3552..3878 /gene="4" /locus_tag="SEA_EVCARA_4" CDS 3552..3878 /gene="4" /locus_tag="SEA_EVCARA_4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /translation="MGIEITDYVPPVKPRKESPYDPVIADLAQYDDGTKAATLTESTE NFSKELRKFREAARHAGFRTRERSRTEDAKAQTVSVSLTIHKRKEKAEAAEVSTDNAD EGESED" gene 4053..4364 /gene="5" /locus_tag="SEA_EVCARA_5" CDS 4053..4364 /gene="5" /locus_tag="SEA_EVCARA_5" /codon_start=1 /transl_table=11 /product="hypothetical protein" /translation="MPLPDYLASLADLEAYPDTLIPDITAEYDTDIAAQVSVITEKDV EIARLMAVNTALQEVNTQLIASVGVAPSDNNDNPDNTPDGDDDTPDEGDISYDDLLES E" gene 4370..5803 /gene="6" /locus_tag="SEA_EVCARA_6" CDS 4370..5803 /gene="6" /locus_tag="SEA_EVCARA_6" /codon_start=1 /transl_table=11 /product="major capsid protein" /translation="MVIAATRPRRFKQFNNIEWLNAFRKTATPEYQSRIPVATQANYD TVLRNLDNYIPGQNAVLAALINKVGLTKFNADHFDNPMAEFIKGTLEYGESVEDVQLG MIQASSYDTDRETGEQIVFSREDIELDSSYYEINRQDRYKVTLDNMGGEIRKAFYNME SGLSDFLNSAMAALQTSANYDEFMLMANLISHYENMGGFFKVHVPDLKTGTRDERKAA AEILLESLREFAAEFAYPNRLYNASGMPTFAKPEKLIILTTPAGKAALDVQALAYMMQ IDKADVPSRIVMIPSNKWKIPGAQAILTTTDFFQVYNVLNTMTEQANPAALYTNHWLH IHQIIAYSRFVPAVLFWTGESTVTDEISWTVTGLGATTAADLDGTSFAELERGGVYEL STTATGTGEGLPTDVGYRLAQPGTSRNTRVGKSGVLLIGHDEYAEEIVIERFSVADTD IRGNQAFPLTGPILDEWPVKETVDPTP" gene 5878..7983 /gene="7" /locus_tag="SEA_EVCARA_7" CDS 5878..7983 /gene="7" /locus_tag="SEA_EVCARA_7" /codon_start=1 /transl_table=11 /product="tail knob protein" /translation="MDMSFSSEFAYNLWTPNTELTFANVPWSNTASDVWYPDVPKSDI RTAFNHYINTLPGIRTINNVNVARPNEPIVVDLLFTEVQQYNYIRATNNVQAGVEGSK TMSYYYFILGAKHTATHTTELVLQLDVWHTFLEWFDIGNAYLEQGHYAVAAENAFQNY GRDFLTVGEGLDYGSEYRIVDTVQRSLITTNGSYSLLVGSTIDLLGDLGTSEKTAKTP SAKASKVQGLISGVAFYVFDTPDSFLTYMENMSLKSWATQGIVSITAIPTLFSWVNGW ADEPAVPALANAGRIAPAAKIPHRTEDGFTNWRNAILDAIPARYRKFTKFLTSPYCMV EMTTYTGQPLMLKPESWRDPNARYRIAISILPPGQRAVFYPVGYNRNTDVDRQAVYTE SLRGDDEGEYLDVATTVSGFPSFAMVNNSAITVLASNQGGLSFAQQAADWSQQRNLEA NRVGYEQAGSGIDLATQLTELANSGIRESRGISDAHAIWGAVSDTAAGIPNFAIGSSL TAGVNAALASSEANNQAALEQRQNTAASRLTGQNADYLRDTNKKLADWGAKGDHALAI AAIDAKTRDINMIPPSVVGQVGGDQFNMVASATGVGMSLRFKLIDDGAIRRIGEHWIR YGYQVHQRITNVGSLHCMSHFTYWKMREVYLVNTNLLEGHKNIIAGMFEKGFTVWKDP NNIGRIDMGINAPLEGITL" gene 7980..8936 /gene="8" /locus_tag="SEA_EVCARA_8" CDS 7980..8936 /gene="8" /locus_tag="SEA_EVCARA_8" /codon_start=1 /transl_table=11 /product="portal protein" /translation="MSGKGNDTVSKIIGQHLTGVAARYLNQRSDNNADLFYSDNFRAL AEMAQNRFEWKNLPATISEEYIEKTLFYKGCVVFFRDRPTRTNAFFALQGHGTGNLNM YDEHTRYQITAPNFRDTSLLSDQCVPIYTNTSRVPDALRASMYANKLTELDVTFGINA QQTRRTRTVVTDANGRMTTAAIIDAIDRGDPVIELTRGLEKDQFDTIDFGVEPKALID LHIARQRIWAEAMTKLGVDNANQDKHERLVSDEVDANNAQIGAMRHSPLQQRRKAVAL INRRYDLDIEVNYRPMETDTGTDDSTPSGDNGEENANGDLHD" gene 8917..9630 /gene="9" /locus_tag="SEA_EVCARA_9" CDS 8917..9630 /gene="9" /locus_tag="SEA_EVCARA_9" /codon_start=1 /transl_table=11 /product="lower collar protein" /translation="MAIYTIDLWELVEHYGLAWLNEYPLEYEDHRDALNRMILDTYWN REIGVETPSMFFVSFRNHMSLNMPVFNELYKSQRIQFDPMITTKLETTTDATTESSSA TSGTSESKSLATETSESVSSSNSEGMSVSYAFPQTRLADRKDYATSAGDSEQESTNTA DSSGSSNRTDEGSDSRNTTTEGADKAKSVTVGYQGVPADLINAFRATIINVDQMVINS LEPLFMGIWGTNSRRIERR" gene 9632..11668 /gene="10" /locus_tag="SEA_EVCARA_10" CDS 9632..11668 /gene="10" /locus_tag="SEA_EVCARA_10" /note="Pre-neck appendage protein" /codon_start=1 /transl_table=11 /product="pre-neck appendage protein" /translation="MANSVVLTGLVLNLFTRALNPTRPLVYEDNLTIIRMLGELEKWI NKNLVEGLNLEVSELIGSINEYRDQLQESIDNYQNEISGELNVLDSSVTARLVENLAT VTAAINEVKDNSIELQDAVLAGILDNVESLAREKADTLYVDKSVVDSGRLSETEVDAT IDARIGGRINVATYPTIQDAIDAAPDGSTLHFPSSMSPVETATGFTLKSNNLTIDAMG VEFRVTQWGVPLFLAARENNGADGHTFRIGMVRYTGVRGNHLGATIRGSAPYCSGCAV WTNGDRNYFEYVRTDGMPTPIYLSSWDGVTVSDRIGVGNRIGYLEAARYNFALLFVAQ RGWDFGNAYCHDDLDDSNGANPTHAIYGSASENFRSPGGTLGRWLVENNNSGAPFQFK FTDGIKADILTANSSAGIASIQNCHDLAVNAMIGYNVKPAITGSRIIELVGAQGCKRP NIGRILVDKAPADKGESLILILDEGGFIGSILVTSRNTVDVGAEIAVRGVGTFTIPAI LVTALNVPARPVRIGNGTEAGKAVGWNMPNVRSVGGGTTYDPVPVEEYTQGHSNSWGD GNSYLAAAAPTRGIFRRGMKWSNAAPATGQPRGWTQVTNGAHAKADWTAATPVAIGDF LAISNGAVIRYYVAGNTGATQPAAVAVGDRGTDGTAAFEVVSLSRATVVSDGVL" gene 11670..12314 /gene="11" /locus_tag="SEA_EVCARA_11" CDS 11670..12314 /gene="11" /locus_tag="SEA_EVCARA_11" /codon_start=1 /transl_table=11 /product="endolysin, protease M15 domain" /translation="MKALKNHPGMYLRDDAADAINALEDKYGTIRINRATVSEAVQQT LIDRWNKGGAANRPPFLFQPKSPAKKSEHVQAIAVDVYNYTSDRAKLEEFGFRWYGPT DPVHYTFVGWNRPATNTPTPAPEEKDEDMANKALGHYVGKGAEVPAEQRTVLIYWPDS GLWTMFSGADQAYINARATQACEGNFTTISVDEYTRYLRPNLKALSGVEPPAVR" gene 12378..13010 /gene="12" /locus_tag="SEA_EVCARA_12" CDS 12378..13010 /gene="12" /locus_tag="SEA_EVCARA_12" /codon_start=1 /transl_table=11 /product="endolysin, protease M23 domain" /translation="MAEPEKVFITGNTMRLIMDDGSQYVANPTPSQVWVFTGGSGGGT DPDPGGSSGFRVPFSWDAVTSEYGPRSGGAGSFHEGTDFGAGLVGGPGTPIITVAGGV VEAEYYHRNFGNMIIVNHGVFSVGPYAGMRLRTLYAHMENPALFEQGQIVPKDAVLGG VGNTGGSFGAHLHLEVHVSGPNSGIVWNTSNNGNYRTAVNARDFFAAYGG" gene 13007..14074 /gene="13" /locus_tag="SEA_EVCARA_13" CDS 13007..14074 /gene="13" /locus_tag="SEA_EVCARA_13" /codon_start=1 /transl_table=11 /product="terminase" /translation="MNMVKLKELPWYNFDKICSYNAIQNYIVGSRGEGKTYGAIKRSI KKFIRHNEEFVYLRRTDTELKPSISSFFRALEIKNEFPDYDFRTTTMMGVHVGQIAHK STRDHDKRVWNTMVYFLALSKAQSYKSVNFLNVTTIIYDEFIKEKGFTHYIPNEFEAF MGLYSTIDRNYDKTVVFFLANAVSIMNPYFIALDIQPDLQREFGRYAKDSQGRYYVAV HISDNKDFKAAVGKTRFAQFSAGTSFGEYAIGNTFRDSHQELIRKKTSTARYRYSLET AKGSFAVWQDFEENLFFLSAKQPKVPTWLTTVPENMSDTKRLVTPTHKLLSSLRTAFT HGRAYFDMASTRNAFAQVFNK" gene 14074..14403 /gene="14" /locus_tag="SEA_EVCARA_14" CDS 14074..14403 /gene="14" /locus_tag="SEA_EVCARA_14" /codon_start=1 /transl_table=11 /product="hypothetical protein" /translation="MRGKCTVSSDDPIMQTRLDVAEMKGMLTQIVSTHEARLLAHDVR LDTHDTRLNEKAKTLARHDEKISDTEEDVKQLQSDNTARLGRNLGVGGLIVAIIGIIL NASKFLP" gene 14419..14655 /gene="15" /locus_tag="SEA_EVCARA_15" CDS 14419..14655 /gene="15" /locus_tag="SEA_EVCARA_15" /codon_start=1 /transl_table=11 /product="hypothetical protein" /translation="MNTPILPNETPNIVIQNPDRRRIIRTVIDSIGGLTFIATAVDAA APEIDITAWTFPILAGYAAARAVFGFTVDNPNTP" gene 14668..14922 /gene="16" /locus_tag="SEA_EVCARA_16" CDS 14668..14922 /gene="16" /locus_tag="SEA_EVCARA_16" /codon_start=1 /transl_table=11 /product="hypothetical protein" /translation="MKRLPAIIPPELIREGDTISVKLPRERGLTIHHEGTVASIEDYA GGRFFKTAEGTRIAVYVPGRNNQARYTLIARKEQEQVSLW" gene 14916..15056 /gene="17" /locus_tag="SEA_EVCARA_17" CDS 14916..15056 /gene="17" /locus_tag="SEA_EVCARA_17" /codon_start=1 /transl_table=11 /product="hypothetical protein" /translation="MVTVAVGFVAFGIGCFLGHANGKWKERERRRRAISAYLDAHDTL DN" gene 15107..15256 /gene="18" /locus_tag="SEA_EVCARA_18" CDS 15107..15256 /gene="18" /locus_tag="SEA_EVCARA_18" /codon_start=1 /transl_table=11 /product="hypothetical protein" /translation="MESEYRNHADELQAIATLAIKDRAMAYWTDPANFPTETDRLFWQ IVKAS" gene 15253..15597 /gene="19" /locus_tag="SEA_EVCARA_19" CDS 15253..15597 /gene="19" /locus_tag="SEA_EVCARA_19" /codon_start=1 /transl_table=11 /product="hypothetical protein" /translation="MNPGYIALAAVLALAVGIAVWFPLWQRRTINTVRYVIINESTHQ ACFGGKRFRSRWKAVKYTQARIPINFQTLFRYSTRAVIKRNGKWQAFGDHPVAIRPAH VQRNPFLRGFGR" gene 15599..15748 /gene="20" /locus_tag="SEA_EVCARA_20" CDS 15599..15748 /gene="20" /locus_tag="SEA_EVCARA_20" /codon_start=1 /transl_table=11 /product="hypothetical protein" /translation="MPVRIHNISKYQCTKCTYGTYDEMLINSHILDNLCKNGSTSYYE RKVTD" gene 15748..15906 /gene="21" /locus_tag="SEA_EVCARA_21" CDS 15748..15906 /gene="21" /locus_tag="SEA_EVCARA_21" /codon_start=1 /transl_table=11 /product="hypothetical protein" /translation="MGASENRAKSAKMAADLKARGIFHGKRTTTPHHNNIPTDAAGSA AYRRLKRK" gene 15977..16156 /gene="22" /locus_tag="SEA_EVCARA_22" CDS 15977..16156 /gene="22" /locus_tag="SEA_EVCARA_22" /codon_start=1 /transl_table=11 /product="hypothetical protein" /translation="MANNETHTVVIGADTEYFNSAEEAADRGYELSKLNPQSRICRYR NGTLVSAYSPKMGWW" ORIGIN 1 gggatgtcac gggattccct gtacaaaaaa ttcccccaat ccttgtaggg ttcctacaaa 61 ggattggggg aggcagtgtg ttgtggggtg tctacaatgt gagtgtagca tacctacaac 121 ttgtaggata gtcacaacca acactgtcaa tcgcgttaac ggttgtgcat ggccaatctg 181 tgtatagtga tatcagcagg aaccgaaagg cacccgctcc cgctcgctgg ggtttcgcag 241 cggtgacaac ttaataggct agcgaagatt gacaaagcca cacagtaacc aagcaatcag 301 ggtggggtta cggctagcgg gtttggcgct ccaaatgaaa cgcgcgacat tgcatgacta 361 gctagttcaa tggggattga tagtgtagga ttgcagtaca atttaacaac acggctagca 421 acagtagaac atctcaccct ttgatatttg ttcggctaga gtcggcggaa ttctgcgctt 481 gagaatgtac tttcgtggcc gacacacttt caatcaccgc ctaactaaag gaaacaaaat 541 gtccaacgaa gttgcagtca tcgagcagac cccgggttcc aacctccgcc cgttcacgtc 601 ggtcgagggg tccgacatgg agacgcggtt ctcgctcgcc gcggccgtca tgggtgcgac 661 cccgctcgcg gatcacaagg gtgttccgtt caacctgcgt cacatcgtgc gtcagccggt 721 ggaggtcgtg aaccagcaga ccaaggctaa gtcgatcacc taccggacca cgctcatcac 781 ggacgacggt cgtgcgttct ccgcaacgtc gcgcggtatc gacgtggccg tggacaacat 841 cctcgcgacg ctcggtaacc cgaaggagtg gggcgactcg tccttcccgt tcatcgctga 901 ggagcgcggt agcggcacgt cgaagttcct gaccctcgtg cccgtggtgg cgaagggcaa 961 gaagtaacac atcagctaag gccgtccccg tttggtacgg gggcggcctt tactgtacct 1021 atagatcatg ctccctggga ttgtagctct acgtgcagca gcactaaaga aacatgcaaa 1081 tgcggtcagt aaggtgtcac gacttaaggc aaggggtgtt gacgttgcgg gtaccgagtt 1141 cgatcctcgt aggccgtggg caacggttaa acggtacaac tcgaaacagc ttgaatcgta 1201 catggaacga ttggacaggt tcaatgcgcg aacgtttcga ctcaacgcgc ttgcagatcg 1261 aaagccgctc ccgctcgggc ggtgggaaca gtatcagcga acggaacgcc agtacaaccg 1321 caaggttgat tcggcgctag atcgcattgg cgatttggtc cctcccggtt ccggcctctc 1381 actacgtcaa cgtgaggaca tgcgtaaatc tgtcaatggc acagcgaaca ctgagaacac 1441 cattctgcac acgaccaacc gtaagcctca atatgtcaaa gacatgaatg cgcttgaaaa 1501 acttgaggcg caactggtaa ggcgaatgtc tgacgagtat cagataggcc agattcagtc 1561 atcgagagtg tccgcggaaa agatggtgga cgccattgga gcgcaagaca ttaacgacgc 1621 actggccgaa ctttctgacg agcaattcga tatcttgtgg agtcgcacac gattccgtga 1681 cactctcatc gctgtgtatg tagaggatga ggatagcaag gataatacac atgtcggtta 1741 cgaccttgac gacgctagac agctcatcag ttgggcgaaa ggtctccgcc tctaagaaag 1801 gcaaagagtg gcttgctgcc gactttgaga ctactacaga cccttttgac gttcgggttt 1861 gggcttgggg cgtcgcacgc gtttcaaacc ccaatgacgt tgtaatcgac aattcaatag 1921 agtcattcat cgatttcatt tcgatggatg actctattac gttcttccat aacctacggt 1981 tcgatggtct gttcatcctt gaccatattc ttaaccaggg attcacgtac gtcgaaggtc 2041 gcgccaatcc gaaaccgaaa gagttcaagg cgcttatctc agataagaat aagttctact 2101 caatcacggt gtgttgggag aacggcgtaa tcactgagtt ccgcgattca gctaaaaagt 2161 tccctggcat gtccatttct cgaatcgcaa agacatttgg actagaggaa tcgaaaggca 2221 ctatcgacta tcacgctccc cggccagtag gacatcagat cacgcccgag gaacgcgagt 2281 acctgaatga cgatgttgtc attctcgcta aggctttgca gatcacaaaa gagacaggtc 2341 tagtcaagct aaccgttgcg tcggacgcga aggctgagta tctgtcacta gttggcgaca 2401 aactgtatga ggcacgcttc ccggtacttt cgtacgcgat ggatgccgaa ctacgacgcg 2461 cataccgtgg cggattcacg tatgccgatc cgcgattctt gcagcagcgt acggggtcgg 2521 gaattgtcct agacgtcaac tcgctctatc cgtacatcat gtctgacagg ccacttccgt 2581 acggcgaacc tgagtacgta ccgggtgagg tcgaaacgac gccagaacgt cccctagcgg 2641 ttttcagcat cacgtttacg gcgcgcctga aagccgatca tatcccgtgc attcagatta 2701 agggcacatc catgttcgcc cccactgagt acctttctgt catcgatgag ccgacaactc 2761 tgatgatgac caacattgac ttccagcttt atcaagacca ttacgatatc gaaatcctcg 2821 aatatcacgg gggctggaga ttcaaagccg cccgcggaat gttcgatgtg tacattcaga 2881 aatgggcaaa gattaaagcc gaaagcacag gcggcttgcg ggagctagcc aaattgcagc 2941 ttaactcttt gtacggcaaa ttcggcgcaa accctaacgt gcgcggaaaa atcccaaccc 3001 tcaccgatgg aaaagtcaag cttgaattgg gcgcccctga tgtacgggat tcgatgtacg 3061 ttcctatcgc cgtgtttgtt acctcatacg gacgcgacct gaccattcgt gcaggtcaag 3121 agaattacga cacattcgcc tacgccgata ccgactcatt gcacttgctg agagatacgg 3181 ttcctgacaa catcgaagtt gacgataagc gcatgggcgc atgggcactc gaatacgcgt 3241 tcgatcaagc gttctacgtc cgcgcaaagg tctacctaga acacatcccc ggtatcgatc 3301 ctgactgcga tgaccctgat tgcacagccc gccacgacta tcacaatgcg ttcgcgggag 3361 tccctgaccg tatctcaaat cgcttgacgt tcgacgacgc ctggaacggg aacatcttta 3421 caggcaagat gatccctcag attgtccctg gcggcgtcgt cctaaaagat gtgccatacg 3481 aattgaaatt ctgaatcagt atggtgtagt gttggcttat cggaaacacc gaccaaacta 3541 aggagaaaat catgggcatc gaaatcacgg actacgtccc gccggtcaag ccgcgcaagg 3601 agtccccgta cgaccccgtc atcgcggacc tcgcacagta cgatgacggc accaaggccg 3661 cgaccctgac cgagtccacc gagaacttct cgaaggaact gcgcaagttc cgtgaggccg 3721 cgcgccacgc cggtttccgc actcgagagc gttcccgcac cgaggatgcc aaggcacaga 3781 ctgtttcggt cagcctgacc atccacaagc gtaaggaaaa ggctgaggcc gctgaggttt 3841 ccaccgacaa cgccgatgag ggcgagtcgg aggactgaga atcgcgatac caccggaccg 3901 acctagaacg aatatcacca aggcgcaacg gttgaacccg cttggtgcta ttgtgggaac 3961 gcatcccacc tagtaggcgg tgagtgtccg ataagatggg cgctaccctt aacggggtag 4021 cgcccattct catatacgaa caggaaaaga caatgccact tcccgattac ctcgccagtc 4081 tggcggacct tgaggcgtac ccggatactc ttatcccgga tatcaccgcc gaatatgaca 4141 ccgacattgc tgcacaggtt tctgtcatca ctgaaaagga tgttgaaatc gcgcgcctta 4201 tggcggtgaa cacggcacta caggaggtta acacgcagct tatcgcaagt gtcggtgttg 4261 caccttccga caacaacgac aaccccgaca acaccccgga cggcgacgac gatacgccgg 4321 acgagggcga catttcatac gatgatctac tggaaagtga gtaacaacaa tggttattgc 4381 agccacgcgc ccacggcgct ttaagcagtt caacaatatc gaatggctca acgccttccg 4441 aaagactgca acccctgagt accagtcccg tatcccggtt gccacgcaag caaactatga 4501 cacggttctg cgcaatctcg acaactacat ccccggtcag aacgccgttc tcgcggcgct 4561 gatcaataag gttggactta ccaagttcaa cgccgaccat ttcgataacc ctatggctga 4621 gttcatcaag ggtacccttg aatacggcga gtcggttgag gacgtgcagc ttggtatgat 4681 ccaggcgagt tcgtacgaca ccgaccggga aaccggcgaa cagatcgtat tctctcgcga 4741 ggatatcgaa ctcgacagtt cgtactacga aatcaaccgc caggaccgtt acaaggttac 4801 ccttgacaac atgggcggcg agattcgcaa ggctttctac aacatggaaa gcggcctttc 4861 ggacttcctg aattcggcaa tggccgcact tcagacttcc gcgaactacg acgaattcat 4921 gcttatggcg aacctgattt cgcactacga gaacatgggc ggattcttca aggtgcatgt 4981 tcccgacctc aagacgggaa cgcgcgatga gcgcaaggcg gcagcagaaa ttctgcttga 5041 gagtctgcgc gagtttgcgg ctgagtttgc ttacccgaac cgtctttaca acgcatcggg 5101 aatgccgact ttcgccaagc cggaaaagct catcatcctt acgacccctg ccggtaaggc 5161 agcgcttgac gtgcaggcgc tcgcatacat gatgcagatc gataaggctg atgtgccgtc 5221 gcgcatcgtc atgattccgt cgaataagtg gaagattcct ggtgcacagg caatcctcac 5281 gactacggac ttcttccagg tctataacgt cctgaacacc atgaccgagc aggccaaccc 5341 tgccgcgctc tacactaacc actggctgca catccaccag atcatcgcgt attcgcgctt 5401 tgttccggca gtgcttttct ggacgggaga atccaccgtg actgacgaaa tctcgtggac 5461 tgtcaccgga cttggtgcca cgaccgccgc ggacctggac gggacgtctt tcgcggagct 5521 tgagcgcggt ggcgtttacg agctgtccac gaccgccacc ggaaccggtg agggtctgcc 5581 gaccgatgtt gggtaccgtc tcgcacagcc cggaacgtcg cgcaacacgc gcgtgggtaa 5641 gtctggcgtc ctgctcatcg gacatgatga gtacgctgag gaaatcgtca tcgagcgatt 5701 cagcgttgcg gacaccgaca ttcgcggcaa ccaggcgttc ccgcttacgg gcccgatcct 5761 tgatgagtgg ccggtcaagg agacggttga ccccacgccg taaggtgtag gctgtcaaag 5821 aaaacccctt ccccgatcca atcggggaag gggttttcgc atgctatggt ggcctttatg 5881 gacatgtcat tttcgagtga attcgcatac aacttgtgga caccgaacac cgaacttact 5941 ttcgctaacg ttccctggtc gaatacggct agcgatgttt ggtacccgga tgtacctaag 6001 tcggatatcc gcacagcttt caatcactac atcaatactc ttcccgggat tcgcacgatc 6061 aacaacgtga acgttgcacg cccgaatgag ccgattgttg tagaccttct ctttacggaa 6121 gtgcagcagt acaactacat tcgtgcgacc aataacgttc aagcgggcgt cgaaggtagt 6181 aagacgatga gctactacta tttcatcctt ggtgcaaagc atacggccac gcacaccacc 6241 gagcttgtac tacagcttga cgtatggcac acgttccttg aatggttcga tatcggtaac 6301 gcgtatcttg aacaaggaca ttacgccgta gcggctgaaa acgcattcca gaactacggc 6361 cgcgacttcc ttacggttgg ggaaggactt gactacggca gtgaataccg cattgttgac 6421 acagtgcagc gttccctcat cactacgaac ggttcctact cgctactggt tgggtcaacc 6481 attgatctac ttggcgattt gggcacgtcg gaaaagacag ctaaaacacc gtccgctaaa 6541 gcgtcgaagg tgcaaggcct gatttcgggt gtagcattct acgtgttcga tacccccgat 6601 tccttcctca cgtacatgga aaacatgtcc ctcaagtcgt gggcaacgca aggcattgtc 6661 agcatcaccg cgatccccac actctttagt tgggtgaacg gttgggcgga tgaaccggcg 6721 gttcccgcac tggcaaacgc ggggcgtatc gcgccggccg caaagattcc ccatcgtacc 6781 gaggacggtt tcacgaattg gcgtaatgcc attctggacg ccattcccgc acgttaccgg 6841 aagttcacca agttccttac ttcgccgtat tgcatggtcg aaatgacgac gtatacggga 6901 cagccgctca tgctcaagcc ggaatcgtgg cgtgacccta acgcacgtta ccgcatcgct 6961 atttcgatcc tcccgcccgg acagcgggca gtgttctacc ccgttggtta caaccggaac 7021 accgacgtag accggcaggc cgtctacact gagtctttgc gcggcgacga tgagggcgag 7081 taccttgacg tggcaacaac ggttagcggt ttcccgtcgt tcgctatggt aaacaactca 7141 gctattaccg tgctcgcatc taaccagggc ggtttgtcgt tcgcccaaca ggccgcggac 7201 tggtcgcagc agcgcaacct agaggcaaac cgcgttgggt atgaacaggc cggttcaggt 7261 attgaccttg cgacgcagct aaccgagctt gccaatagcg gcattcgtga gagtcgcgga 7321 atttccgatg cccacgcgat ttggggtgct gtcagcgata ccgcggcagg tattccgaac 7381 ttcgctatcg gtagctcgct tacggcaggt gtaaacgctg cactggcgtc gagtgaggca 7441 aacaaccagg cagctttgga gcagcgacag aacacggcgg catcgcgtct caccggtcag 7501 aacgctgact acctgagaga cacgaacaag aaacttgctg attggggtgc aaagggcgat 7561 cacgcgctag caattgcagc tatcgacgca aagacgagag atatcaacat gattcccccg 7621 tcggttgttg ggcaggtcgg cggcgatcag ttcaacatgg ttgcaagtgc aaccggcgtg 7681 ggaatgagtt tgcggttcaa gctgattgac gacggcgcta ttcgtcgcat tggtgagcat 7741 tggattaggt acgggtatca ggtacatcag cgcattacca acgtcggcag cctgcattgt 7801 atgagccact ttacgtactg gaaaatgcgg gaagtgtatc ttgtgaacac gaaccttttg 7861 gaaggacaca agaatatcat tgctggaatg tttgaaaagg gattcaccgt gtggaaggat 7921 cctaacaaca tcggtcgtat cgacatgggc attaacgccc cactggaagg aattacgctg 7981 tgagcggcaa gggaaatgac actgtctcaa agattatcgg gcagcatcta acgggtgttg 8041 cagcacggta tctgaatcag cgttccgaca acaacgcgga tttgttctat tctgacaact 8101 tccgtgcact agccgaaatg gcgcaaaaca ggtttgagtg gaagaatctt cccgcaacga 8161 tcagtgagga atacatcgaa aagacactgt tctacaaggg ctgtgtggta ttcttccgcg 8221 accgtcccac acgtacaaac gcattcttcg cgctacaggg acatggcacg ggcaacttga 8281 atatgtacga tgagcacact cgatatcaga tcaccgcgcc caacttccgt gatacaagtc 8341 tgttgtctga ccagtgcgtg ccgatctaca cgaacacatc gcgggtgccg gacgctttgc 8401 gagcatcaat gtacgcgaac aaacttaccg agcttgacgt tacgttcggc atcaatgcgc 8461 agcagacacg gcgaacccgt acggttgtaa ccgacgcaaa cggccgcatg acaaccgcgg 8521 ccattatcga cgctatcgac cgaggcgatc ccgttatcga gcttacgcgc ggactcgaaa 8581 aagaccagtt cgacacgatc gattttgggg tcgagcctaa agcactgatt gatttgcaca 8641 ttgcacggca acgcatttgg gctgaggcta tgaccaagct cggtgtagac aatgcgaatc 8701 aggataagca tgaacggctt gtatctgacg aggttgacgc gaacaacgca cagattggcg 8761 ctatgcgaca ctcgccgttg cagcagcgtc gtaaagcggt tgcacttatc aaccgccgat 8821 atgaccttga cattgaggtc aattaccggc cgatggaaac cgacacggga actgatgaca 8881 gcacccctag cggtgacaat ggagaggaaa acgccaatgg cgatctacac gattgacttg 8941 tgggagcttg tggagcacta cggcctagca tggctcaatg agtacccgct tgaatacgag 9001 gaccatcgtg acgcgctaaa ccgtatgatc cttgacacat actggaaccg tgaaattggg 9061 gtcgaaaccc cttccatgtt ctttgtgtcg ttccgtaatc acatgagctt gaacatgccc 9121 gtgttcaatg agctttataa gagtcaacgg attcagttcg atccgatgat tacgaccaag 9181 ctggaaacga ctacggacgc tacgactgag tcgagttccg ccactagtgg aactagcgag 9241 tccaagtcgc tcgctaccga aacatcggaa tccgtaagtt ctagcaactc tgagggaatg 9301 agcgtatcct atgcattccc gcaaacgcgc cttgcagatc gtaaggatta cgccactagc 9361 gcgggagaca gtgaacagga aagcacgaac actgccgaca gtagcggtag cagcaatcgg 9421 acggatgagg ggtccgatag ccgcaatacc acgactgagg gcgcggacaa ggcaaagagt 9481 gttactgtcg ggtatcaggg tgtcccggca gacctgatta acgcgttccg cgcaacaatc 9541 attaacgtag accaaatggt aatcaattcc cttgaacccc ttttcatggg gatttgggga 9601 actaattccc gtcgcatcga aaggcgataa catggctaac agcgtcgtac ttacagggct 9661 tgtgctcaac ctctttacga gggcactaaa ccccacgcgc ccgcttgtgt atgaggataa 9721 ccttacgatt atccgcatgc ttggtgagct cgaaaagtgg attaataaga accttgtgga 9781 aggtttgaac ctggaagtta gcgagctgat cggctctatc aatgagtacc gcgatcagtt 9841 gcaggaatct atcgacaatt accagaacga gatttccggc gagcttaacg tacttgacag 9901 ttccgtcact gcccgactgg ttgagaacct ggcgaccgtc accgcagcga ttaatgaggt 9961 caaggacaat tccattgagc tacaggacgc cgttctcgca ggcattcttg acaatgtcga 10021 atcgctcgcg cgtgaaaagg ccgatacgct ctacgttgat aagtcggtcg tggacagcgg 10081 tcgactttcg gaaacggaag ttgacgcaac aatcgacgcc cgtatcggtg ggcgcatcaa 10141 tgtcgcaaca tacccgacca ttcaggacgc tatcgacgct gcacccgatg gtagtacgct 10201 gcactttccg tcatccatgt cgccagtcga aaccgcaacc ggtttcacgc tcaagtctaa 10261 caacttgact attgacgcaa tgggggtcga gttccgcgtc acacagtggg gagttccgct 10321 gttccttgcg gcacgtgaga acaacggcgc ggacggtcat accttccgta tcggaatggt 10381 gcgttatacg ggtgtccgtg gcaatcacct tggtgccacg attcgaggtt ccgcgcctta 10441 ctgttccggc tgtgctgtgt ggacaaacgg cgacagaaat tatttcgagt acgttcgtac 10501 cgacggaatg ccgaccccta tttacctttc gtcgtgggac ggcgttaccg tttccgaccg 10561 catcggcgtt ggtaaccgca ttggatatct tgaggctgcg cgatacaact tcgcacttct 10621 ctttgtcgca cagcggggtt gggacttcgg aaacgcgtac tgccacgatg acctggatga 10681 ttccaatggc gcaaacccta cgcacgctat ctacggttcg gcatcagaaa acttccgatc 10741 ccctggcggc acgcttgggc gttggcttgt agagaacaac aattccggcg cacctttcca 10801 gttcaagttt acggacggaa tcaaggccga cattctgaca gctaacagtt ccgcgggtat 10861 cgcgagcatc cagaattgtc atgacctcgc cgtgaacgct atgatcggtt acaatgtcaa 10921 gcctgcaatt accggttccc gcatcatcga gcttgtgggc gcacagggat gcaagcgccc 10981 caacatcggc cgtatcctgg tcgataaggc tccggcagat aagggcgaat cgcttatcct 11041 cattcttgat gagggcggct ttatcggatc tattctggtc accagccgga acacggtgga 11101 tgttggtgct gaaatcgcag tacggggcgt cggcactttc acgattcccg caatcctcgt 11161 cacggctttg aatgttcctg cacgaccggt acgcatcggt aacgggactg aagcgggcaa 11221 ggccgttgga tggaacatgc ctaacgtgcg ttccgttggt ggcggtacta cctacgatcc 11281 cgtgccggtg gaagagtaca cacagggaca ctcgaattcg tggggtgatg gtaacagcta 11341 cctggcagca gcagcgccta cccgcggtat cttccgtcgt ggcatgaaat ggtcgaacgc 11401 tgcacccgct accgggcagc cacgcggatg gacgcaggta acgaacggcg ctcatgctaa 11461 ggccgattgg acagcagcaa caccggtcgc tattggtgac ttcctggcaa tcagtaacgg 11521 cgcagtgatt cgctactatg ttgcgggcaa cacgggagct acacagcccg cagccgttgc 11581 cgtgggcgac aggggtaccg atgggaccgc cgcttttgag gtcgtttcgc tgagccgcgc 11641 aacggtcgtt tccgacgggg tgttgtgaca tgaaagcact aaagaatcat cctggcatgt 11701 atctacggga tgatgctgcc gacgcgatta atgcgctgga agataagtac gggactatcc 11761 gcatcaaccg ggcaacggtc agtgaggcgg tacaacagac actgatcgat cggtggaata 11821 aaggtggtgc ggcaaatcgt ccaccattcc tattccagcc gaaatcgccc gccaagaaat 11881 ccgagcatgt gcaagctatc gcggttgatg tttacaatta cacaagcgac cgcgccaagc 11941 tggaagagtt cggattccgt tggtacggac ctaccgaccc cgtacactac acattcgtag 12001 gatggaatcg tcccgcgact aacaccccca caccagcacc ggaagaaaag gatgaggata 12061 tggcaaacaa agcgctaggg cattatgtcg gcaagggtgc tgaggtaccc gccgaacagc 12121 gaacggtttt gatctactgg ccggattcgg gcttgtggac gatgtttagt ggcgcagatc 12181 aggcgtacat caatgcccgc gccacacagg catgtgaggg aaatttcaca acgatcagcg 12241 tggacgagta cacacgctat cttcgcccga acctgaaggc gcttagcggg gtcgaaccgc 12301 ccgcggttcg ataaggtaca atgagaggcc gggacgtttt cgtcccggcc tttcgcctac 12361 actgaaagac ggtaatcatg gccgaaccgg aaaaggtttt cataaccggc aacacaatgc 12421 ggctcatcat ggatgacgga tcgcagtacg tcgccaatcc cacaccctcg caggtatggg 12481 tattcactgg cggttccggt ggcggcaccg accccgaccc cggcggcagc agcggtttcc 12541 gtgtcccgtt ctcgtgggat gccgtgacca gcgaatacgg tccccgtagc ggtggagccg 12601 gttcgttcca cgagggaacc gacttcggtg caggactagt cggcggacct ggaacaccga 12661 tcatcacagt tgccggtgga gttgtagagg ctgaatacta tcaccgcaat ttcggtaaca 12721 tgattatcgt gaaccatggc gtctttagtg tcggacccta cgcgggcatg agacttcgca 12781 cactctacgc gcacatggaa aatcccgcac tattcgagca agggcagatc gtgccgaagg 12841 atgccgtgct aggcggggtc ggtaacacgg gcggatcgtt cggtgcgcac ttgcatttgg 12901 aagtacatgt ctccggtccg aattcaggca tcgtgtggaa cactagcaac aacggaaact 12961 accgtacagc cgttaacgcg cgcgatttct tcgcggcata tgggggatga acatggttaa 13021 gctaaaagag ttgccttggt acaacttcga caaaatctgt tcgtacaatg cgatccagaa 13081 ctacatcgta ggatcgcgtg gtgagggtaa gacgtacggg gcgatcaagc gcagcatcaa 13141 gaaattcatt cgccacaatg aggagttcgt atacctgcgc cgtaccgaca ccgaacttaa 13201 gccgtcgatt tcgtcattct tccgcgcgct cgaaatcaag aatgagttcc cggattatga 13261 ctttcgcact acaacaatga tgggtgtgca cgtcgggcaa attgcacaca agtcaacgcg 13321 cgatcacgat aagcgcgtgt ggaatacaat ggtgtacttc ctggcactgt cgaaagctca 13381 atcttacaag tcggtcaact tcctcaatgt aacaacaatc atttacgatg agttcatcaa 13441 ggaaaagggt ttcacgcatt acatcccgaa cgaatttgag gcgttcatgg ggctgtattc 13501 cacgattgat cgcaactacg ataagacagt cgttttcttc ctggctaatg ccgtgtcgat 13561 tatgaaccct tactttatcg ccctggacat tcagcctgac ctacagcgag agttcggacg 13621 gtacgcgaaa gacagtcaag gccgttacta cgtcgctgta cacatttccg acaacaaaga 13681 ctttaaggca gcggtcggta agacgcgatt tgcgcagttc tctgcgggca cgtcatttgg 13741 tgaatacgct atcggaaata ccttccgtga ttctcaccaa gaattgatcc gcaagaaaac 13801 cagtactgcc cggtatcgct actcattgga aaccgctaaa ggcagctttg cagtgtggca 13861 agacttcgag gaaaacctat tcttcctttc cgctaagcag cctaaagtac cgacatggct 13921 tacgaccgtg cctgagaata tgagcgatac caagcgactt gtgaccccga cacataagct 13981 gctatcgtcc ctcagaacgg cgttcactca cggtcgagca tatttcgata tggcgtccac 14041 acgtaacgcg ttcgcgcaag tgttcaataa gtaatgaggg gaaaatgtac cgtgagtagt 14101 gatgatccga taatgcaaac taggctagac gttgctgaaa tgaaaggcat gttgacgcaa 14161 attgtcagca cacatgaggc gcgactgcta gcgcatgatg tgcggttgga tactcacgac 14221 acacgcctta atgagaaggc taaaacgctt gcccgacatg acgagaagat cagtgacact 14281 gaggaagatg taaagcagct acagtcggac aacacggcac gccttggtcg caaccttggc 14341 gttggtggtc tgattgtcgc tatcatcgga atcatcctta atgcctcaaa gttcctacca 14401 tagaaaagag aaacgaaaat gaacacgccc attcttccca acgaaacccc caacatcgtc 14461 attcagaacc ccgaccgtcg ccgcatcatc cgcacggtga ttgacagcat cggcgggctc 14521 acgttcatcg caaccgctgt agacgcagcc gcaccggaga tcgacattac cgcgtggacg 14581 ttccccatcc tcgctgggta cgccgcggca cgtgccgttt tcggcttcac ggtggacaac 14641 cccaataccc cgtagtagga tcgcagcatg aaacggctac cggctatcat ccctcccgaa 14701 ctcatccggg aaggcgacac tatcagcgtc aaactcccac gggaacgcgg gcttacgatt 14761 caccatgagg ggaccgtagc tagcatcgaa gattacgccg gtggccgttt ctttaagaca 14821 gcggaaggaa cgcgtatcgc cgtatacgtc cccggccgca acaaccaagc tcgatacacc 14881 ctcattgcac gtaaggaaca ggagcaggtt tcactatggt gacagtcgca gtcggctttg 14941 tagcgttcgg catcggatgc tttcttggcc acgctaacgg gaagtggaag gaacgcgaga 15001 gacggcgacg tgccatatct gcgtatctag acgcccatga cacactcgac aactaaatga 15061 gaaaccccgg cttgtacagc cggggttttc atgtatactg aggggtatgg aaagcgagta 15121 ccggaatcac gccgatgaac tgcaagccat tgcgacactt gcaatcaagg atcgtgctat 15181 ggcgtattgg acggaccccg cgaactttcc taccgaaacc gaccgcctat tctggcagat 15241 tgtgaaagca tcatgaatcc cggatacatc gccctcgcgg cagtcctcgc tctcgccgtg 15301 ggaatcgccg tttggttccc cttgtggcag cgccgaacga tcaacacggt acgatacgtc 15361 atcatcaacg aaagcacgca tcaggcgtgt ttcggcggta agcgtttccg ttcgcgctgg 15421 aaggccgtga aatacactca ggcgcgaatc cccatcaact tccaaacact gttccgatac 15481 tcgacacggg cagtcatcaa gcgcaacggt aagtggcagg cgttcggcga tcaccccgta 15541 gccatacggc cagcgcacgt acagcgaaac ccgttccttc gcggattcgg gcgctgacat 15601 gcccgtacga atccacaaca tcagtaagta ccagtgcacc aaatgcacct acggtaccta 15661 cgatgaaatg ctcatcaact ctcacattct cgacaatctg tgcaagaatg gtagcaccag 15721 ctactacgaa cgaaaggtaa ccgactaatg ggtgcatccg agaaccgcgc gaagtccgcc 15781 aaaatggctg ccgacctcaa ggcgcgtggc atcttccacg gcaagcgcac gacgacgccg 15841 catcacaaca acatccccac cgacgccgcg ggcagcgccg cataccgtcg cctcaagcgg 15901 aagtagaaac cggatacgcg aaatcgccta gtgaaaaaca ctaggcgatt ttgcgttcat 15961 gcggtacaat tgaaacatgg caaacaacga aactcacaca gtcgttatcg gcgctgacac 16021 ggaatacttc aattcagccg aagaggcagc agatcgtggc tatgagcttt ccaagctcaa 16081 ccctcagtca cgaatctgcc gttaccgtaa cggcaccctc gtttccgcat actcccctaa 16141 aatgggttgg tggtgaacac cgtgagttgt aggtatgcta cactcacatt gtagacaccc 16201 cacaacacac tgcctccccc aatcctttgt aggaacccta caaggattgg gggaattttt 16261 tgtacaggga atcccgtgac atccc //