LOCUS Piku 15675 bp DNA linear 30-AUG-2024 DEFINITION Arthrobacter phage Piku. ACCESSION VERSION KEYWORDS . SOURCE Arthrobacter phage Piku ORGANISM Arthrobacter phage Piku Viruses;dsDNA, no RNA stage;Caudovirales;Podoviridae. REFERENCE 1 (bases 1 to 15675) AUTHORS Kamal,Y., Fatima,S., Slakeski,N., Syeda,M., Perez,M.T., Klyczek,K., Ko,C., Russell,D.A., Jacobs-Sera,D. and Hatfull,G.F. TITLE Direct Submission JOURNAL Submitted (30-AUG-2024) Biology, University of Wisconsin-River Falls, 410 South Third Street, River Falls, WI 54016, USA COMMENT Phage isolation, DNA extraction, and annotation analysis performed at Benedictine University, Lisle, IL Sequencing by Illumina to approximately 6511x performed at the Pittsburgh Bacteriophage Institute, Pittsburgh, PA Assembly performed using Newbler and Consed as of February, 2023 Supported by Science Education Alliance, Howard Hughes Medical Institute, Chevy Chase, MD This genome has defined ends with 15-base 3' overhangs (CCACGGTCCCCGTCC). FEATURES Location/Qualifiers source 1..15675 /organism="Arthrobacter phage Piku" /mol_type="genomic DNA" /isolation_source="soil, Schaumburg, IL" /lab_host="Arthrobacter globiformis B-2979" /country="USA" /lat_lon="42.034004 N 88.100311 W" /collection_date="01-Oct-2022" /collected_by="Bisma Khan" /identified_by="Bisma Khan" /note="complete genome" gene 175..1761 /gene="1" /locus_tag="SEA_PIKU_1" CDS 175..1761 /gene="1" /locus_tag="SEA_PIKU_1" /codon_start=1 /transl_table=11 /product="terminase" /translation="MSEVPTDTDITNAEIGIRMLGLTLFPQGRDVAAVMEAKSTEIDM LTGRPAPLYAHCTVQEPRRSSKTTAIQAVYLGRCETIPGHRVVQTAQDGTRASGFFMN MVRMLERVTPEPADRNWKVYKSTGREYLEWSNGSRWWVVPPDPAAFRGEAADDIWFDE GGEFKPETAKELRNGVLALMDTRSDAQITISGTPGIVRAGMFWEDLEAARKDPNALGI VDYSAADNDLLVLPDGTPNEDLWWLRHPGLACGLTTIKKMRERWEKLGPVGFGQEYLC IWSSDNTISALDPVQWVDTTTEPVPFAGQDFDLTFDCHIQGAFSAIVATWLDDAGEVH AQVMDYRPGIDWVAAELARANRAHPKVKINYDAIGNNSAVAMSLQRIPGFKINALRPV TMRDAAAGAALVSSGLANGTWHHALSPALDTAAQNVTWRYSGESRLFGRKSANVCVAA VVAASIGTAMTTGRRRRKTGSLPAPIFGNDVPVPDVPVPTSGDPFPDGFGSPDDPVPN EAVSVTPANRLRQLVAAGRI" gene 2066..3196 /gene="2" /locus_tag="SEA_PIKU_2" CDS 2066..3196 /gene="2" /locus_tag="SEA_PIKU_2" /codon_start=1 /transl_table=11 /product="portal protein" /translation="MGLTDRISNILGFPSRPGLFDGVAVRSPESALLESVVFFGGGNV QMPVTSRTALKVPGIARAIQLYTAVCAQLPLKASTESADSKWLNWSDGTISPALRNAL TVQDIIFHGGSCYAVARNDAGHVTNGIRIPVGFWTVDAAGRIQVNTALTDPDAKDGPH RFVDVDQKQIMYIPSLMPCGLLDMADDGIQTYLQIGRTIKDRASNPTPLIALVVKDDA VADPDELKTAQTNWHKARTDPNGAVAIVPAGVGIETPGADRDDSAMLIGARNASRLDV ANWLNLPAAMLDGNSGTSDQYSNTLQNQNEFLALSVSLPIRAIEARLSQDDVTPAGVT VSFDTAVFDSMPEPATGNTGAAVEPSPAGAAVETEQERELTA" gene 3193..4737 /gene="3" /locus_tag="SEA_PIKU_3" CDS 3193..4737 /gene="3" /locus_tag="SEA_PIKU_3" /codon_start=1 /transl_table=11 /product="major capsid and protease function protein" /translation="MTIQVVGDLLTANDDTLTASYTLLTFGEPGRTNKGTITVDPGVL PVPAGALPVNDEHDPAVSVGYMLAAEAPERITTDVHFYRTPAGEAAYKAAKSGDKRGI SMEVLEPVIRAGKLLAGRLVAAGTVKKPAFPSSLLTAAEDTGDVSPELQTALDNLAAA AAAVQAAATNPPADPPADPPADANNNPTTDKVTASMSNPPAVPPLLAGNTGNTAGPDM SFAALATALAGGKKSPELLAAFATVTEANVFDVATPPTYVGEITAQSTYKRRYADLVT NNPLTGSKVQGWRFVEGKTPVVRPYAGDLAEIPSNEVVLEEVSFDAGRFAGGNKVDRK HYDFPTPGFWAGYLRESTGDYDKQIDLLVRDTLLNTTNEILAGASIAGVAEGWSKLVD GALAVWDFGTPDWALVGVDLYREMALTSEKDRLAFLNASLGLDEAAAAGFRIRPVSGP GTDGTVTVGTKGGVELQELPGLIRVSAVDVSHAGVDEGVYGYGGVFTRDARAIRKVVD TLTPAP" gene 4740..5063 /gene="4" /locus_tag="SEA_PIKU_4" CDS 4740..5063 /gene="4" /locus_tag="SEA_PIKU_4" /codon_start=1 /transl_table=11 /product="head-to-tail adaptor" /translation="MLVGWVDYDPTKELWADSILAGEDKVRELLENAFESCVIYAPKL PDGAPVPQRYKDAQVLQARAIWQMQRQGPGDQFGADGISIAIYPLDARIRQMLRPKQV LGGML" gene 5060..5440 /gene="5" /locus_tag="SEA_PIKU_5" CDS 5060..5440 /gene="5" /locus_tag="SEA_PIKU_5" /codon_start=1 /transl_table=11 /product="tail terminator" /translation="MSLRSDLAAVLKNELGTGFAVLASKRAIDNTSIPVVMVQRKTVT PGPERKRLSTDVEVLVLVAETYGDGAEDKADEALDDVLRVLERIEDPVVWSRAERDNF EGGFVGYVVTLEATTDNYLLPGRE" gene 5447..5836 /gene="6" /locus_tag="SEA_PIKU_6" CDS 5447..5836 /gene="6" /locus_tag="SEA_PIKU_6" /codon_start=1 /transl_table=11 /product="major tail protein" /translation="MPALFLKNATVTVDGADASEDADNVEFTPTTTAATFTPISGTTQ SDSGATSWVCGMNIAQDFTAGSLFMIMYEADEPVDVVLKPRGTASGPTITATIVPVPA KIGGGAGALTATVSCQVIGKPAITPTV" gene 5840..6367 /gene="7" /locus_tag="SEA_PIKU_7" CDS 5840..6367 /gene="7" /locus_tag="SEA_PIKU_7" /codon_start=1 /transl_table=11 /product="Hypothetical Protein" /translation="MVVRVQPSVEALDAYKAVVLAMKVIDKPIRKAINVDTRTTLSPV WKKLVTEHAGTLLDQRVLNTGTRIAAGNPPAAIAGASKRRLSGGLVPAEYNRMVEFGV DPKDKNVPSTYSRKTKTGKTSTVKRRTRIGLPNARDKGRVVYPAFADFAPRAIAYWVQ SVVRITHETLEKGSR" gene 6369..8084 /gene="8" /locus_tag="SEA_PIKU_8" CDS 6369..8084 /gene="8" /locus_tag="SEA_PIKU_8" /codon_start=1 /transl_table=11 /product="tape measure protein" /translation="MAAGINLPFFADVRNFLKGTDSIADALDDVAGSLDEIDEASKAS AEKAGDAIASGFEDGAKDASRAADKLERDVSDNMTGIASDAKKAGNKAGDAIKDGMKD GTSAADRLEQKVSDTFRGVAADAKAAGDKVGKSMKDGSDRAGEGLDDMKSEAASTAKE TAASFDGSADSIVGSFQEVAANAFAAFGPAGLAAGLAAAAGIGVAMTVLQGVADEAND TGDAVTDMATKIRDAGGDMSKVDLTEGMIDYGFAIQDTKEWFEFFQDSAETGFEQIKK KSEEAGVSWVSAFKGTKGSAEDSRTALADVVEKLEDARENATMWVDAASGMQGVDLAD QKKIDALEDLKKKFETNIETVERAESANRDLAAAGIKTTEQIEAERDAVEAANESLLA HRDALDAAAGAAIDADKAELDYVKTLADGNADIKKNGETVDINTEKGRANRQTLLDMA GAANSLINAQIEQGDSTAAVTTKTQQARDSFIRSAEAAGYSKDAAKKLADQYGLIPKN VATKVEARNVEKTKREIDGVAAPRDVPINLTRGTESVSSWLRGLSGRTIPVNIAVRGG RGVTD" gene 8100..9509 /gene="9" /locus_tag="SEA_PIKU_9" CDS 8100..9509 /gene="9" /locus_tag="SEA_PIKU_9" /codon_start=1 /transl_table=11 /product="minor tail protein" /translation="MATITAELLPNSAAIRLSLNVAAGIKSIIRRDANGINDVRTGPG VFGIVPELAFAGTNLIKNPSFETGISGVGKVGTTVTLTASTYAPGLVVGAGGLMVDVG AGANSNTYIFQTVDVTAGNWLAAALNVRANSDYLGSVRLGIMFYDSTGASIAGSLTKS APFDPPSNSTGGARYSFSALAPANAKTANVYVYSYGDTAGSTAPAAGTRYFTDAWAAC ESATQAQAEYMIAQYFDGSYAPSLEFKTAWTGTAHASTSTRSVPASAVVVTDYEAASG PVRYDVIDLNDRLESLDVAGFALDAPWLFTPLIPGYSRKAVSVTGLDTEFEDRSTVHS GLLGRPDPVVVLRPLGLRSGTMEIYAGTYADALEILSPLQRATVMMLRQPEHAGLDMY FAPAGGSPKILSLVTDGGRTVWGVQVPYVEVKRPEGMIAGALGWTYADLAAAVPRYSD LPRTFATYADMRINKRITP" gene 9506..10633 /gene="10" /locus_tag="SEA_PIKU_10" CDS 9506..10633 /gene="10" /locus_tag="SEA_PIKU_10" /codon_start=1 /transl_table=11 /product="minor tail protein" /translation="MTLTGLYQDRALDVVKQTHRQDGAATLHFPTGDPLDIILRDPQI TFSEDWSPYMQLTADAVTPADAETLVRIDPRNVGAGAVLEVRAGYVYDDGTADVQTLA LGHLRSRRALLPEGTMPVTAASAEQLAQDTKWLLATTTKVFGGVLEALEYLTTYATGK NTAGTFISTINPVHRPDLVSGVVLEQGEDLWGPISAIALSAGLRVWADENDVWNLAPK TTIAGVTSAFLKQGPATTVSKVEDVLSRENWFNAAALVYTWKDSGGVEQKIVGTWSPD PAPGTEKGAGCKTFTDDRPGPISQYQANENARLTVKNLSTRGGSYVVESVAMFWLRPG MTVQIDLANGVTVRHIVRRVTFNVGAGSMILTTREPSNLGD" gene 10643..11041 /gene="11" /locus_tag="SEA_PIKU_11" CDS 10643..11041 /gene="11" /locus_tag="SEA_PIKU_11" /codon_start=1 /transl_table=11 /product="Hypothetical Protein" /translation="MATTTKRSYRYPGQNAEPDAAGDVQRLAEDVDNDVDKLFAGLPD KILAGSDSISIGAGAATITKVITFPTAFAAAPTITVQNTANVAGRASLLNLYVTAKSA TQFTVKMQTSDNANAGTSYAISFDWIAVSA" gene 11038..11766 /gene="12" /locus_tag="SEA_PIKU_12" CDS 11038..11766 /gene="12" /locus_tag="SEA_PIKU_12" /codon_start=1 /transl_table=11 /product="endolysin" /translation="MTVFCRPARGRETQPFATIQLQDGLPHAGTDFGYYDAAGLPRPE VFAAEAGTVLFAGDSRSLGWPNPWYFNPDFDRTDDRDSSAGNVLVIGHRAGVTTYSHL AGFKVTKGMTVTRGQHVATIGDTGNANGKHLHFEWIPYPFDFGTATYGRVRPTFRKGL FMYLNEKQELEVLAAARAINLRAKYLDAPVSAVPKKAAAALLDAPIPRKGGRSGITTP RNVFAYSDANLDAADDMPETDPAA" gene 11763..12017 /gene="13" /locus_tag="SEA_PIKU_13" CDS 11763..12017 /gene="13" /locus_tag="SEA_PIKU_13" /codon_start=1 /transl_table=11 /product="membrane protein" /translation="MTGGTTPAPVNPARFIPSPRLRAYLYGILVPAGALLVFRGIITA GELGLWLSLAGAVLAVSNGLALANTPANTAKGSRNDTAGR" gene 11998..12306 /gene="14" /locus_tag="SEA_PIKU_14" CDS 11998..12306 /gene="14" /locus_tag="SEA_PIKU_14" /codon_start=1 /transl_table=11 /product="Hypothetical Protein" /translation="MTPPVDEITTGELGRRLDAFGTTIQDGFRELSKKIDDRPDWQDV RRIESGLVERITAESEARKVAQGIADRAILALEDGQKWATRLILGAVGLGVINLIWTR " gene 12477..12746 /gene="15" /locus_tag="SEA_PIKU_15" CDS 12477..12746 /gene="15" /locus_tag="SEA_PIKU_15" /codon_start=1 /transl_table=11 /product="helix-turn-helix DNA binding domain protein" /translation="MTNKDKPPGAADEARIVVPSDLISTGEAAKILGVDRATIVRRAR TGKIPIVAQLDADTGHGAYVFDRNEVKGTGEDPKNGMAHNGTGSD" gene 12727..12912 /gene="16" /locus_tag="SEA_PIKU_16" CDS 12727..12912 /gene="16" /locus_tag="SEA_PIKU_16" /codon_start=1 /transl_table=11 /product="helix-turn-helix DNA binding domain protein" /translation="MAQAATRVGVSVRTVERYVDAGKLDAHKLPSGRRRVRIGDVDAL LRPVRRPAGDAAKVGSK" gene 12909..13529 /gene="17" /locus_tag="SEA_PIKU_17" CDS 12909..13529 /gene="17" /locus_tag="SEA_PIKU_17" /codon_start=1 /transl_table=11 /product="helix-turn-helix DNA binding domain protein" /translation="MSWEVLAWAMKKGRDYQLQPTTRHVMLTLANYADPEGNDIYPSL SRLELDTGLSERTIRRQIQHLMGCRLLDYGDQRVVEQNPRIRPDQRPKVYRFILEAVD NSAERPDTMTTRGYGFAPKVVDNSGHARTLSPERPDKRPVTVSTEPINQKLKPGAGFA DEAADAVLDVLPTVSGSDFVEEIRRRRAEREAEKQKEALDHARSRG" gene 13510..14052 /gene="18" /locus_tag="SEA_PIKU_18" CDS 13510..14052 /gene="18" /locus_tag="SEA_PIKU_18" /codon_start=1 /transl_table=11 /product="Hypothetical Protein" /translation="MHDHAANDAVLPSQRITMNNTTLRDDLRPAAADEYVPAAVIHDH VEAGGFGWPVSGDLTTIEYGIFHDLGFARTDPKPRTGITSGRLSPIVATTSTLGALWN LDAAELNAKVRSGSTAPVAVDGDGHLRARAALIASNEAMNKAWADAREECFATDPNAS RRRDEARDRYRKALAAARSV" gene 14055..14396 /gene="19" /locus_tag="SEA_PIKU_19" CDS 14055..14396 /gene="19" /locus_tag="SEA_PIKU_19" /codon_start=1 /transl_table=11 /product="HNH endonuclease" /translation="MEDMLFDIPGLVKAPEKAKGRRWGGNDSAKARAIVAPTLPRPCT RCGKTVTADMKWHADHIVEDVFGGESTPDNLGPAHAHCNTSAGGKIGAAMTNGFKQAQ DQRREVTVKFW" gene 14393..15031 /gene="20" /locus_tag="SEA_PIKU_20" CDS 14393..15031 /gene="20" /locus_tag="SEA_PIKU_20" /codon_start=1 /transl_table=11 /product="DNA methyltransferase" /translation="MRVLVACEYSGAVRDAFTRAGHDAMSCDLLDTEVPGNHYTGDVR DVLDDGWDLMVCHPPCTDLAVSGNAHMAKKWADGRVDAALDFVRLLLSAPIPRIALEN PVAIISSQIRKPSQTIQPWEYGHPEKKATCLWLKNLPPLTPTKIVYDHMMTLPKKEAE RVHMLPPSDTRWLERSRTFPGIAAAMADQWGPLGPFDPAVEGDLLDLLALTN" gene 15071..15454 /gene="21" /locus_tag="SEA_PIKU_21" CDS 15071..15454 /gene="21" /locus_tag="SEA_PIKU_21" /codon_start=1 /transl_table=11 /product="Hypothetical Protein" /translation="MERSDLEADVVGAALAGDKVVVLADTRDDVRPIFQTIADAFTGD DGKVMDGVTIRKQNGRERIELRNGSRITFGSVRRPDSLRGLVADRIYTPAETKVEVLR ILEAVTITSPKPATLLYGIRIQADA" gene 15451..15591 /gene="22" /locus_tag="SEA_PIKU_22" CDS 15451..15591 /gene="22" /locus_tag="SEA_PIKU_22" /codon_start=1 /transl_table=11 /product="Hypothetical Protein" /translation="MTVPVATALAVLILILAMIAAGFAGWWLARLVAWLWETRRGRLT RR" ORIGIN 1 gcgccgtttc cgcgcgtaga cgtgctataa gatgcgaatt agtacgcttc aacaattcgc 61 gaagggatcg cacaaatgga aactctcgaa cgggtctttt gggatcacac ccgggaccgg 121 ctcgttggtc ctcccggcgg tccgctgaca atttcaaaga gccggccgcg gtttatgtcc 181 gaagtcccga cagacacgga tatcacaaac gccgaaatcg ggatccggat gctcgggctc 241 acattgttcc cccagggccg ggacgtcgcc gccgtgatgg aagcgaagtc gacagagatc 301 gacatgctca ccggccggcc ggcgccgctc tatgcacact gcacagtcca ggagccgcgc 361 cggtcctcga agaccacagc aatccaagcc gtgtatctcg gaaggtgcga gacgatcccc 421 ggacaccggg tcgtccagac ggcacaggac gggacacgtg cgtccgggtt tttcatgaac 481 atggtccgga tgctcgaacg cgtcacgccg gagccggccg atcgcaactg gaaagtgtac 541 aaatcgaccg gccgcgaata cctcgaatgg tccaacggat cccgttggtg ggtcgtcccg 601 ccggacccgg ccgcgttccg cggcgaagcg gccgacgaca tttggtttga cgaaggcggg 661 gagttcaagc cggagacggc gaaggaactc cgcaacggcg tcctcgctct catggacacc 721 cgttcggatg cacagatcac gatctcgggg accccgggga tcgtccgggc cggcatgttt 781 tgggaagacc tcgaagccgc caggaaggac ccgaacgcgc tcgggatcgt cgactactcc 841 gcggcggaca acgacttgct cgtcctcccg gacgggacgc ccaacgaaga cctctggtgg 901 ctccgacacc cgggcctagc ctgtggtttg acgaccatta agaagatgcg cgaacgttgg 961 gaaaagctcg ggccggtcgg cttcggtcaa gagtatttgt gtatctggtc cagtgataac 1021 acgatctccg cgctcgaccc cgtccaatgg gtcgacacca caacagagcc ggtccccttc 1081 gccggccagg acttcgacct caccttcgac tgccatatcc agggagcttt ttccgcgatc 1141 gtcgcgacgt ggctcgacga cgccggcgaa gtccacgcgc aggtgatgga ctaccggccc 1201 gggattgatt gggtcgcggc cgaactcgcc agggcgaacc gggctcaccc gaaagtaaag 1261 atcaactatg acgcgatcgg gaacaactcc gcggtcgcca tgtccttgca gcggatcccc 1321 ggtttcaaga tcaacgcgct ccggccggtg acgatgcgtg acgccgcggc cggcgccgct 1381 ctggtgtcct ccgggctcgc caacggcacg tggcaccatg ccctctcgcc ggcgctcgac 1441 acggcggcgc aaaacgtgac gtggcgatac tccggcgaat cccggctctt cggccggaag 1501 tctgccaacg tgtgtgtcgc cgcggtcgtc gcggcgtcga tcggcacagc catgacgacc 1561 ggccggcgcc ggcgtaagac gggatcgctc cccgcgccga tcttcgggaa cgacgtcccg 1621 gtcccggacg tcccggtccc gacgtcgggg gatcccttcc cggacggctt cgggagcccg 1681 gacgacccgg tccccaacga agcggtctcc gtgacgccgg cgaaccggct ccggcaactc 1741 gtcgcggccg gccggatctg atccccggga aacctcgaga attttggagg tttcgtcaag 1801 ccggcctttt ttgagatccg cggattctag gcaactttcg gtcgccggcc ttttggtttc 1861 gttgtgggga tccgacaaac ccggcgccgg agccggcccg ggactcccga caaaccgcga 1921 caaacccggc cataacctcg acaattcgat aaagcgtttg gatgaaaagc gccggcggtc 1981 ccgaaatcgg ggtcgccggc gctttttgtg tggtgtcatt tggttgttcg attcccccaa 2041 atgaccgaat ggagaataag cccggatggg acttaccgac cgaatctcta acattctcgg 2101 atttccgtcc cgtccgggcc tattcgacgg ggtcgcggtc cgctccccgg agtcggcttt 2161 actcgaatcc gttgtttttt tcggcggcgg aaatgtacaa atgccggtca cgtcgcggac 2221 cgcgctaaag gtccccggga tcgccagggc gatacagctc tacaccgccg tttgtgctca 2281 actcccgctg aaagcgtcga ccgaatccgc cgactcgaag tggctcaatt ggtcggacgg 2341 gacgatctcg ccggcgctcc ggaacgcgct cacggtacag gacattattt tccacggcgg 2401 atcctgctac gccgtcgcca ggaacgacgc cggccatgtc acgaacggga tccggatccc 2461 ggtcggcttt tggacggtcg acgccgccgg tcggatccag gtcaacaccg cgctcacgga 2521 cccggacgcg aaggacggtc ctcaccgctt cgtcgacgtc gaccaaaagc agatcatgta 2581 tatcccatca ctgatgccgt gtgggttgct cgacatggcg gacgacggga tccagacgta 2641 tttgcagatc ggccggacga tcaaagaccg ggcttcgaac ccgactccgc taatcgcgct 2701 cgtggtcaaa gacgacgcgg tcgcggaccc ggacgaactg aaaacggcac aaaccaattg 2761 gcacaaagcc cggacggatc ccaacggcgc ggtcgcgatc gtccccgccg gcgtcgggat 2821 cgaaaccccg ggagcggacc gcgacgattc ggcgatgctg atcggagccc ggaacgcgtc 2881 ccggctcgac gtcgccaact ggttgaacct tcccgcggcg atgctcgacg ggaactccgg 2941 gacgtccgac caatactcga acacgttgca gaaccaaaac gaatttcttg cgctctcggt 3001 ctctctgccg atccgggcga tcgaagcccg tttgagtcag gacgacgtca cgccggccgg 3061 cgtgaccgtg tccttcgaca ccgccgtgtt tgactccatg cccgaacccg cgaccggcaa 3121 caccggcgca gcggtcgaac catccccggc cggcgccgcg gtcgaaaccg aacaggaaag 3181 agaactcacc gcatgacaat ccaggtagtc ggcgatctgc tcaccgccaa cgacgacacc 3241 ctcacggcgt cttacacgct gctcaccttc ggcgaaccag gccggaccaa caaagggacg 3301 atcacggtcg accccggggt cctaccggtc cccgccggcg ctctcccggt caacgacgaa 3361 cacgatcccg ccgtctcggt cggttacatg ctcgccgccg aagctccgga gcggatcacc 3421 acggacgtcc atttctaccg gaccccggcc ggcgaagccg catacaaagc ggcgaaatcc 3481 ggggacaaac gcgggatctc gatggaagtc ctcgaacctg tgatccgtgc cgggaagctg 3541 ctcgccggcc ggctcgtcgc ggccgggacc gtcaagaaac ccgctttccc gtcctcgctg 3601 ctcaccgcgg ccgaagacac cggcgacgtc tcgcccgaac tgcaaaccgc gcttgacaac 3661 ctcgcggccg cggccgctgc tgtccaggcc gcggccacga atccgccggc ggatcctccc 3721 gccgatccgc cggcggacgc caacaacaac ccaacaacag acaaggtaac cgcaagcatg 3781 tcgaaccctc ccgccgtccc gccgctgctc gccggcaaca ccggcaacac cgccggcccg 3841 gacatgtcct tcgccgctct cgcgaccgct ctcgccggcg gtaagaagtc gccggaactg 3901 ctcgccgctt tcgcgaccgt gaccgaagcg aacgtcttcg acgtcgcgac cccgccgacc 3961 tacgtcgggg agatcacggc acagtcgacg tataagcgcc gttacgccga cctcgtgacc 4021 aacaacccgc ttaccggctc gaaggtccag ggatggcgct tcgtcgaagg taagacgccg 4081 gtcgtccggc cctacgccgg ggacctcgcc gaaatcccgt ccaacgaagt cgtcctcgaa 4141 gaagtgtcct tcgacgccgg ccgcttcgcc ggcggtaaca aggtcgaccg gaagcactac 4201 gatttcccga ccccgggttt ctgggccggc tacctccggg agtccaccgg cgactatgac 4261 aagcagatcg acttgctcgt ccgcgacacg ctgctcaaca cgaccaacga aatcctcgcc 4321 ggcgcgtcga tcgccggtgt cgccgaaggt tggtcgaagc tcgtcgacgg cgctcttgcc 4381 gtgtgggact tcggtactcc ggattgggct ctcgtcggcg tcgacctgta ccgcgaaatg 4441 gcgctcacgt cggagaagga ccgtctcgct ttcctcaacg cctctctcgg tctcgacgaa 4501 gccgcggccg cgggtttccg gatccgtccg gtctccggcc cgggaacgga cgggacggtc 4561 acggtcggca cgaagggagg cgtcgaactt caggagctcc ccggactgat ccgggtctcc 4621 gcggtcgacg tctctcacgc cggcgtcgac gaaggtgtct acggttacgg cggcgtcttc 4681 acccgcgacg cccgggcgat ccgcaaggtc gtcgacaccc tcacgccggc gccgtagtca 4741 tgctggtggg atgggtcgac tatgacccca ctaaagagct atgggccgat tccattctcg 4801 ccggcgaaga caaagtccgg gagcttctcg aaaacgcgtt tgagtcttgt gtgatctacg 4861 cgccgaagct cccggacggc gctcccgtac cccaacggta caaagacgcg caggtcctcc 4921 aagcccgggc gatctggcag atgcagcgcc aggggcccgg ggatcaattc ggcgccgacg 4981 ggatctccat tgctatctac cctctcgacg cccggatccg gcaaatgctc cggcccaaac 5041 aagtcctagg cggaatgcta tgagtctccg atccgacctc gccgcggtcc tcaaaaacga 5101 actcgggacc ggcttcgcgg tcctcgcgtc gaaacgggcg atcgacaata cgtcgatccc 5161 ggtcgtcatg gttcagcgga agaccgtcac gccagggccg gaacggaagc ggctctcgac 5221 cgacgtcgaa gtcctcgtcc tcgtcgccga aacctacggg gacggcgccg aagacaaagc 5281 cgacgaagcc ctagacgacg tcctccgggt cctcgaacgg atcgaagacc cggtcgtctg 5341 gtccagggcc gaacgcgaca acttcgaagg cggcttcgtc ggctacgtcg tcaccctcga 5401 agcgaccacg gataactacc tactccccgg aagggaataa ccgaaaatgc cagctctgtt 5461 tctgaaaaac gcgaccgtca cggtcgacgg cgcggacgcg tccgaagacg ccgacaacgt 5521 cgaatttact ccgacgacga ccgcggcgac gttcacgccg atctccggga cgactcagtc 5581 cgattccggc gctacctcgt gggtttgcgg tatgaacatt gcgcaggatt tcaccgccgg 5641 atccctgttt atgatcatgt acgaagcgga cgaaccggtc gacgtcgtcc tcaaaccccg 5701 cgggacggcg tccggcccga cgatcacggc gacgatcgtc ccggtcccgg cgaagatcgg 5761 cggcggcgcc ggcgctctca ccgcgaccgt ctcttgccag gtgatcggca agccggccat 5821 tactcccacg gtctaggaca tggtcgtccg ggtgcaacct tcggtcgaag ccctcgacgc 5881 ttacaaagcc gtggtcctcg ccatgaaggt gatagacaaa ccgatccgca aagcgatcaa 5941 cgtcgacacc cggacgaccc tctccccggt ctggaaaaag ctcgtcaccg aacacgccgg 6001 gacgctgctc gatcaacggg tcctcaacac cgggacacgg atcgcggccg gcaacccgcc 6061 ggccgcgatc gccggcgcgt ccaaacgccg gctctccggc ggactggtcc cggccgaata 6121 caaccgcatg gtcgaattcg gcgtcgaccc gaaagacaaa aacgtcccgt cgacgtactc 6181 ccggaagacg aagaccggga agacctcgac cgtcaagcgc cggacccgga tcgggctccc 6241 gaacgccagg gacaaaggcc gggttgtgta cccagctttc gccgacttcg ctccccgggc 6301 gatcgcctat tgggtgcaat cggtcgtccg catcactcac gaaacactag aaaagggctc 6361 ccgctaacat ggccgctggg atcaatctcc cgtttttcgc cgacgtccgg aacttcctaa 6421 aggggaccga ttcgatcgcg gacgcgctcg acgacgtcgc cggctctctc gacgaaatcg 6481 acgaagcgtc gaaagcgtcc gcggaaaagg ccggcgacgc gatcgcgtcc ggcttcgaag 6541 acggcgcgaa agacgcctca cgcgccgctg acaagctcga acgcgacgtc tccgacaata 6601 tgaccgggat cgcgtccgat gcgaagaaag ccggcaacaa agccggcgac gcgatcaaag 6661 acggcatgaa ggacgggacg tccgcggcgg accggctcga acagaaagtc tccgacacct 6721 tccgcggggt cgcggcggac gcgaaagccg ccggcgacaa ggtcgggaag tccatgaagg 6781 acgggtccga tcgggccggc gaaggtctcg acgacatgaa gtccgaagcg gcgtcgaccg 6841 cgaaagagac cgcggcgtct ttcgacgggt ccgcggattc gatcgttggg tccttccagg 6901 aagtcgccgc gaacgctttc gccgctttcg ggccggccgg tctcgccgcc ggtctcgcgg 6961 ccgcggccgg gatcggcgtc gctatgaccg tgttgcaggg tgtcgcggac gaagcgaacg 7021 acaccggcga cgccgtcacc gacatggcaa cgaagatccg ggacgccggc ggggacatgt 7081 ccaaagtcga cctcaccgaa ggcatgatcg actatggctt cgcgatccag gacacgaagg 7141 aatggtttga gtttttccag gactccgcgg aaaccggctt cgaacagatc aaaaagaaat 7201 ccgaagaggc cggcgttagt tgggtctccg ctttcaaagg tacgaaggga tccgcggaag 7261 actcccggac cgctctcgcc gacgtcgtcg aaaagctcga agacgccagg gaaaacgcga 7321 ccatgtgggt cgacgcggcg tccgggatgc agggtgtcga cctcgccgac caaaagaaaa 7381 tcgacgcgct cgaagacctc aaaaagaagt tcgaaaccaa cattgaaacc gtcgaacggg 7441 ccgaatccgc caatcgggat ctcgccgcgg ccgggatcaa aacgaccgaa cagatcgaag 7501 cggaacgcga cgccgtcgaa gccgccaacg aatccctact cgctcaccgg gacgcgctcg 7561 acgccgcggc cggcgccgcg atcgacgccg acaaagccga actcgactac gtcaagacgc 7621 tcgcggacgg gaacgcggat atcaaaaaga acggggagac cgtcgatatc aacactgaga 7681 agggccgggc gaaccgtcaa acgctgctcg acatggccgg cgccgcgaac tctctgatca 7741 acgcgcaaat cgaacagggc gactcaaccg ctgctgtcac cacgaagacg cagcaagccc 7801 gggactcgtt tatccgatcc gccgaagccg ccggctactc gaaagacgcc gcgaagaaac 7861 tcgccgacca atacggtctg atcccgaaga acgtcgcgac gaaggtcgaa gcacggaacg 7921 tggaaaaaac gaagcgggaa atcgacggcg tcgccgctcc cagggacgtc ccgatcaacc 7981 tcacccgcgg gaccgaatcg gtgtcctcat ggctccgtgg gctctcgggc cggacgatcc 8041 ccgtcaacat tgctgtccgc ggcggccgcg gcgtgaccga ctgaaaaggg ttttagcaca 8101 tggcaaccat taccgcggaa ctactcccga actccgccgc gatccggctc tcgctcaacg 8161 tcgccgccgg gataaagtcg atcattcgcc gggacgctaa cgggatcaac gacgtccgga 8221 ccggtcccgg tgtcttcggt atcgtccccg aactcgcttt cgccggcacg aacctaatca 8281 aaaacccgtc tttcgaaacc gggatctccg gggtcggcaa agtcgggacg acggtcacgc 8341 tcacggcgtc gacctatgcg ccaggactcg ttgtcggcgc cggcggactc atggtcgacg 8401 tcggcgccgg cgcgaacagt aatacctaca tctttcagac cgtcgacgtc accgccggta 8461 attggctcgc cgcggcgctc aacgtccgcg caaactccga ttatctcggc tcggtccggc 8521 tcgggatcat gttctatgac tcgaccggcg cttcgatcgc gggctcgctc acgaagtcag 8581 ctccgtttga tcctccgtca aactccaccg gcggcgcccg ttactctttt tcggcgctcg 8641 ctccggcgaa cgcaaagacg gctaacgttt acgtctattc ctacggcgat accgccggca 8701 gcacagcgcc ggccgcgggg acccgatact tcacggacgc ttgggcggcg tgcgagtcgg 8761 ctacgcaagc acaggccgaa tacatgatcg ctcaatactt cgacgggtcc tatgctccgt 8821 cgctggaatt caagacggca tggacgggga ccgctcacgc gtcgacgtcg acacggtccg 8881 tcccggcgtc cgcggtcgtt gtcaccgatt atgaagcggc gtccgggccg gtccgttatg 8941 acgtgatcga cctcaacgac cggctcgaat ccctcgacgt cgccggcttc gctctcgacg 9001 cgccgtggct cttcacgccg ctaatccccg ggtactcccg gaaagcggtc tccgtgaccg 9061 ggctcgacac cgaattcgaa gaccggtcga cggtacactc cgggttgctc ggacgcccgg 9121 acccggtcgt cgtcctccgg ccgctcggac tccgctccgg gacgatggaa atctacgccg 9181 ggacctatgc ggacgcgctc gaaatcctct ccccgttgca gcgtgcgacc gtgatgatgc 9241 tccggcaacc cgaacacgcc gggctcgaca tgtacttcgc gccggccggc ggatctccga 9301 agatcctctc gctcgtcacg gacggcggta gaaccgtgtg gggagtccag gtcccctacg 9361 tcgaagtgaa acgtcccgaa ggcatgatcg ccggcgctct cggatggacg tatgccgacc 9421 tcgcggccgc ggtcccccgt tattcggacc tcccgcggac cttcgccaca tacgcagaca 9481 tgcggattaa caaaaggatt acgccatgac tctcaccggt ctttaccagg atcgcgcgct 9541 cgacgtcgtc aagcagactc accgccagga cggcgccgcg acactgcact tcccgaccgg 9601 ggatccgctc gacatcatcc tccgggatcc ccagatcact ttctccgaag actggtcccc 9661 gtatatgcaa ctcaccgcgg acgccgtgac gccggcggac gccgaaaccc tcgtccggat 9721 tgatccccgg aacgtcggcg ccggcgccgt cctcgaagtc cgggccggct acgtctatga 9781 cgacgggacc gcggacgtcc aaacgctcgc gctcggacac ctccggtcac gccgggcgct 9841 gctcccggaa gggacaatgc ccgttacggc ggcgtccgcc gaacagttgg cacaggatac 9901 aaaatggttg ctcgcgacga cgacgaaggt cttcggcggg gtcctcgaag ccctcgaata 9961 tctgacgacc tacgcgaccg ggaagaacac ggccgggacg tttatctcca cgatcaaccc 10021 cgtccaccgg cccgacctcg tctccggcgt cgtcctcgaa cagggcgaag acctctgggg 10081 tccgatctcc gcgatcgcgc tctccgccgg gctccgggta tgggcggacg aaaacgacgt 10141 ctggaacctc gcgccgaaga ccacgatcgc cggcgtgacg tccgctttcc tcaaacaggg 10201 gccggcgacg accgtctcca aggtcgaaga cgtcctctcc cgggaaaact ggttcaacgc 10261 ggccgcgctc gtctacacgt ggaaggactc cggcggcgtc gaacaaaaga tcgtcgggac 10321 gtggtccccg gaccccgcgc cagggaccga aaaaggcgcc ggatgcaaga cctttaccga 10381 cgaccggccc ggccctatca gtcagtacca ggccaacgaa aacgcccgtc tcacggtcaa 10441 gaacctctcg acccgcggcg gatcctacgt cgtcgaatcg gtcgctatgt tttggctccg 10501 gcccgggatg accgtccaaa tcgacctagc gaacggcgtc acggtccggc atatcgtccg 10561 ccgcgtgacg ttcaacgtcg gcgccggctc catgatcctt accacccgcg aaccttccaa 10621 cctaggagac tgatccccga acatggccac aacgactaaa cgcagctacc gatacccagg 10681 gcagaacgcc gaaccggacg ccgccggcga cgtgcagcgg ctcgccgaag acgtcgacaa 10741 cgacgtcgac aagctcttcg ccggtctccc ggacaagatc cttgccggct ccgattcgat 10801 ctctatcggc gccggcgccg cgacgatcac gaaggtgatc accttcccga ccgctttcgc 10861 ggcggcgccg acgatcactg tgcagaacac ggcgaacgtc gccggccgtg cgtcgctgct 10921 taatctctac gtcaccgcga aatccgctac acagttcact gtgaaaatgc agacgtccga 10981 taacgcgaac gccgggacgt cttacgcgat ttccttcgat tggattgcgg tgtccgcatg 11041 accgtctttt gccgtcccgc caggggacgg gaaacccaac ccttcgcgac tatccagcta 11101 caggacggtt tgccgcacgc cgggacggac ttcggttact acgatgcggc cgggctcccg 11161 cggccggagg tcttcgcggc cgaagccggg accgtcctct tcgccggcga ctcccggtcg 11221 ctcggatggc ctaatccgtg gtacttcaac ccggatttcg accggaccga cgaccgggac 11281 tcgtccgccg gcaacgtcct agtgatcgga caccgggccg gcgtcacaac gtactcccat 11341 ttggccggct ttaaagtcac caagggcatg accgttaccc gcggccagca tgtcgccacg 11401 atcggcgaca ccgggaacgc gaacgggaaa caccttcact tcgaatggat cccctatccc 11461 ttcgacttcg ggacggctac ctacggccgg gtccgaccaa cctttaggaa aggattgttt 11521 atgtacctca acgaaaagca ggaattggaa gtcctcgccg ccgccagggc gatcaacctc 11581 cgggcgaagt acctcgacgc gccggtctcc gcggtcccga agaaagccgc ggccgcgctg 11641 ctcgacgcgc cgatcccgcg gaagggtggt cggtccggga tcacgacccc gcggaacgtc 11701 ttcgcctatt cggatgcgaa cctcgacgcc gcggacgata tgcccgaaac ggacccggcc 11761 gcatgaccgg cgggacgacg ccggctccgg tcaacccggc ccgtttcatc ccgtccccgc 11821 ggctccgggc ctacctgtac gggatcctcg tccccgccgg cgctctgctc gtcttccgtg 11881 ggatcatcac ggccggggag ctcggtctat ggctctcgct cgccggcgcc gtcctcgccg 11941 tgtcgaacgg gctcgctctc gccaacacgc cggccaacac tgcgaaggga tcgcggaatg 12001 acaccgccgg tcgatgaaat caccacaggg gagctaggcc ggcggctcga cgcgttcggg 12061 acgacgattc aggacggttt ccgggaactg tccaaaaaga tcgacgaccg tcccgattgg 12121 caggatgtcc ggaggatcga atccgggctc gtcgaacgga tcacggccga atccgaagcc 12181 cggaaagtag cgcaggggat cgcggaccgg gcgatccttg cactcgaaga cggccaaaaa 12241 tgggcgaccc ggcttattct cggcgcggtc ggtctcggtg ttatcaattt gatatggact 12301 aggtaagttg tgggatcccg ctaagccggc tcgccaggcg ccagctgtga aaaccgcgga 12361 acggcgggga gttgcggaaa cgggctttgt gtggatccca caacaccggc cggccggcga 12421 cacgcggaat gcgtcaagtg acgcggacgc ggccgaaact gtgcaacgat cgacgcatga 12481 caaacaaaga caagccgcca ggagcggcag acgaagcccg gatcgtagtc ccgtccgacc 12541 tgatctccac cggcgaagcc gcgaagatcc taggggtcga ccgggcaacg atcgtaagac 12601 gcgccaggac cgggaagatc ccgatcgttg cgcaactcga cgccgacacc ggtcacggcg 12661 cttatgtatt cgaccggaac gaagtaaagg gtactgggga ggatcctaag aatggaatgg 12721 ctcacaatgg cacaggcagc gactagggtc ggggtctcgg tccgcacggt cgaacgctac 12781 gtcgacgccg gcaagctcga cgctcacaag ctcccgtccg gccggcgccg ggtccggatc 12841 ggggacgtcg acgcgctcct ccggcccgta cgccgtcccg ccggcgacgc cgcgaaggtg 12901 ggatcgaaat gagctgggaa gtccttgcat gggccatgaa gaagggccgg gactaccaac 12961 tgcaaccgac gacccgtcac gtcatgctca cgctcgccaa ctacgccgac ccggagggga 13021 acgacattta cccttccctc tcgcggctcg aactcgacac cgggctttcc gaacggacga 13081 tccgccggca gattcagcac cttatgggat gccgtttgct cgactatgga gaccaacggg 13141 tcgtcgaaca aaacccgcgg atccggcccg accaacgacc gaaggtctac cgtttcatcc 13201 tcgaagctgt ggataactcc gcggaacgac cggacaccat gaccacccgt ggatacggat 13261 tcgcgccaaa ggttgtggat aactccggtc acgcccggac actgagtccc gaacgaccgg 13321 acaaacgacc ggtcacagtg tccaccgaac ctattaacca aaaacttaaa ccaggggccg 13381 gcttcgcgga cgaagcggcg gacgccgtcc tagacgtcct cccgaccgtc tccggctcgg 13441 acttcgtcga agagatccga cgccgtcgcg ccgaacgcga agccgaaaaa caaaaagaag 13501 ctcttgacca tgcacgatca cgcggctaac gacgccgtcc taccttcgca aaggatcacc 13561 atgaacaaca ccacactccg cgacgacctc cgtcccgccg ctgctgacga atatgtcccc 13621 gctgctgtca tacacgatca cgtcgaagcc ggcggcttcg ggtggccggt ctccggggac 13681 ctcaccacga ttgaatacgg gatctttcac gacctaggct tcgccaggac ggatcctaag 13741 ccgcggaccg ggatcacgtc cggccggctc tccccgatcg tcgcgacgac gtcgaccctc 13801 ggcgctctct ggaatctcga cgccgcggaa ctcaacgcga aagtacggtc cgggtcgacc 13861 gcgccggtcg ccgtcgacgg ggacgggcac cttcgcgcca gggccgcgct gatcgccagc 13921 aacgaagcca tgaataaagc atgggcagac gcccgggaag aatgcttcgc gaccgacccg 13981 aacgcgtccc gccgtcgcga cgaagcccgg gaccgctacc gtaaagccct cgccgccgct 14041 aggagcgtct aacgatggaa gacatgctct tcgatatccc cgggctcgtg aaagcgccgg 14101 agaaagccaa gggccggcgc tggggcggga atgactccgc gaaggcccgg gcgattgtcg 14161 cgccgaccct cccgcggcca tgtacccgct gcgggaagac cgtcacggcc gacatgaaat 14221 ggcacgcgga ccatatcgtc gaagacgtct tcggcggcga atcgaccccg gacaatctcg 14281 ggccggcaca tgcccactgc aacacgtccg ccggcgggaa gatcggcgcc gccatgacga 14341 acggtttcaa gcaagcccag gaccaacgga gagaggtcac ggtgaaattc tggtgagagt 14401 cctagtcgct tgtgaatact ccggagccgt ccgcgacgcg ttcacccggg ccggccatga 14461 cgccatgtcc tgtgatctgc tcgatacgga agtcccgggg aaccactaca ccggggacgt 14521 ccgcgacgtc ctcgacgacg ggtgggacct catggtctgc cacccgccgt gtacggacct 14581 agcggtctcc ggcaacgctc acatggcgaa gaaatgggcg gacggccggg tcgacgccgc 14641 gctcgacttc gtccgcttgc tgctctccgc tccgatcccc aggatcgcgc tcgaaaaccc 14701 ggtcgcgatc atctcgtccc aaatccggaa accctcccag acgatccaac cttgggagta 14761 cggacacccg gaaaagaaag caacgtgtct gtggctaaag aacctcccgc cgctgacgcc 14821 gacgaagatc gtctatgacc acatgatgac actcccgaag aaagaggccg aacgggtgca 14881 catgctcccg ccgtccgata ccaggtggct cgaacggtcc cggaccttcc ccgggattgc 14941 cgcggctatg gcggaccaat ggggaccact cggacccttc gaccccgccg tcgaaggcga 15001 cttgctcgac ctgctcgcac tcaccaacta acaccaacac aacgaagggc acgaaatgag 15061 aatcacaaat atggaacgtt cggatctcga agcggacgtc gtcggcgccg ctctcgccgg 15121 ggacaaggtg gtcgtcctcg ccgacacccg ggacgacgtc cggccgatct tccaaacgat 15181 cgcggacgcg ttcaccggcg acgacgggaa ggttatggac ggcgtgacga tccggaagca 15241 gaacggccgg gagcggatcg aactccgcaa cggatcccgg atcaccttcg ggagcgtccg 15301 ccggcccgac tcgctccgcg gtctcgtcgc ggaccgaatc tatacgccgg ccgaaacgaa 15361 ggtcgaagtc ctccggatcc tcgaagccgt cacgatcacg tccccgaagc cggcgacgct 15421 gctctacggg atccggatcc aggccgacgc gtgaccgtcc ccgtcgcgac cgctctcgcc 15481 gtcctcatcc tgatcctcgc catgatcgcc gccggcttcg ctggctggtg gctggcgcga 15541 ctcgtggctt ggttgtggga gactagacgc ggtcgactaa cgcgaaggta gaccgatcgg 15601 acccggtccg gatccaattt gggggtccgg accgggtctt tttgctgaaa ccgtcaaagc 15661 ccacggtccc cgtcc //