LOCUS RoscoWindex 17453 bp DNA linear 17-JAN-2025 DEFINITION Microbacterium phage RoscoWindex. ACCESSION VERSION KEYWORDS . SOURCE Microbacterium phage RoscoWindex ORGANISM Microbacterium phage RoscoWindex Viruses, dsDNA viruses, no RNA stage; Caudovirales; Siphoviridae. REFERENCE 1 (bases 1 to 17453) AUTHORS Katsanos,J.M., Harmouch,J.C., Delesalle,V.A., Viland,M.D., Lewis,C.M., Garlena,R.A., Russell,D.A., Jacobs-Sera,D. and Hatfull,G.F. TITLE Direct Submission JOURNAL Submitted (17-JAN-2025) Biology, Gettysburg College, 300 N Washington, Gettysburg, PA 17325, USA COMMENT Phage Isolation, DNA preparation and genome annotation performed by Queens University of Charlotte Sequencing by Illumina, 2741x coverage, done at the Pittsburgh Bacteriophage Institute. Assembly performed by Newbler and Consed as of Jan 11, 2024. Supported by the Science Education Alliance, Howard Hughes Medical Institute, Chevy Chase, MD. FEATURES Location/Qualifiers source 1..17453 /organism="Microbacterium phage RoscoWindex" /mol_type="genomic DNA" /isolation_source="soil in Charlotte, NC" /lab_host="Microbacterium foliorum NRRL B-24224" /country="USA" /lat_lon="35.182095 N 80.919029 W" /collection_date="01-Oct-2023" /collected_by="Mykyle Wenceslao" /identified_by="Mykyle Wenceslao" /note="complete genome" gene 41..379 /gene="1" /locus_tag="SEA_ROSCOWINDEX_1" CDS 41..379 /gene="1" /locus_tag="SEA_ROSCOWINDEX_1" /codon_start=1 /transl_table=11 /product="Hypothetical Protein" /translation="MSDTTTTDAEPFELDANSTWIAAVELFQKEGAEWLTAADAPQLM ALRSIAKQLDGGTFQAALISQFTLIHRALLNRRPGEPQQPTDPAGAARQAAMGPSLFE QLGGVWTADA" gene 376..1083 /gene="2" /locus_tag="SEA_ROSCOWINDEX_2" CDS 376..1083 /gene="2" /locus_tag="SEA_ROSCOWINDEX_2" /codon_start=1 /transl_table=11 /product="Hypothetical Protein" /translation="MSRQLHLDSPRYPQPCGDRECRRCYPPTPAEARAAYVAAWDELQ RSDLDRFREAVEQLHAAMVELLLPVFEAFADLVERLAWLIEDSRPVRWLNRRARRAAM RRHPSSRWQFTPITAEAGRQLGLAFGQGVEIAMRREAVTSAEALALRAGIRLPELPPA RIYTPPAALSWVDVDRLAERAGQDLGTFDAILAAPTPAEIPPAPLCQPVNAGQHVGRM RRHTWRELLQLPGRDMA" gene 1086..2546 /gene="3" /locus_tag="SEA_ROSCOWINDEX_3" CDS 1086..2546 /gene="3" /locus_tag="SEA_ROSCOWINDEX_3" /codon_start=1 /transl_table=11 /product="terminase" /translation="MSMSQPYPEIAPWPPRRYTPPLSDDFPSAFDGYRDLFRLIWFAA FGYALEAWQEQAVRHVLELYPAGHRRAGQLRWRQVVISLGRQNGKTEIAAAIGLFALL MKAAPNVVGIASSAEQARLVYKRTMRAIRGTPKLAQRFRALTETRGIQTKDGGQYEIK AAKSAALQGIPIDAGLVDELHILARALWFDLVNGMGGRPNCFVVGITTAGDENSELLL HLYEQGSEAIDAGADARFGFYVWEADAAEIPDDDDELGRELARANPSVASGRVDLENA LIEVRSMPKSDAIRYRLNRFVQSVSEFITAAMWHDNFVREDWPEGVAPIFTIDRTPDW SAATISAFGKRPDGRIYCDVVASIVRPDLARLVELCEQLNAAHRPATFGMDSFTLRDL GRELDLRGIPTTMAGIADVMNGSALFYAKLQQQRLAHPGHDLLAHQIPLTKRKDSDGG FKISRQASSASIDGVMSHVIGVQLAENHRDNALQMF" gene 2671..3798 /gene="4" /locus_tag="SEA_ROSCOWINDEX_4" CDS 2671..3798 /gene="4" /locus_tag="SEA_ROSCOWINDEX_4" /codon_start=1 /transl_table=11 /product="portal protein" /translation="MGRIREWLAGSSRSASSDNPTTGVTAPSRSAALGTVTVADAFGI SMVYRAIQIHAISAKQLSIETTRYGRMVEDHPLTRRPDPQSTRSSWIEQVVVSMASTG NGYAEIVRDAFGQPIALPVLNPLQVRIHTDAAGHVTKYSYRNRELQPRDVMHVTLLRV PGSAYGLGPVQAAQPDLRGAISTRDYAAAWLDDSGVPTGVLKSDQNITRDTAAMAKEH WNENAGQKNGVVVLGQGLDYRPIFLSPKDVQFIESQQFSVTQIARLFGTPASLMLAAV EGNSQSYSNVEQDWLAYVRFTLMGYLTEIEDALSELLPGARRARFNIEALLRADTTTR YASYKTAIEAGFLTVPEVREIEGWAPLPDDAETPSQETPTA" gene 3795..5381 /gene="5" /locus_tag="SEA_ROSCOWINDEX_5" CDS 3795..5381 /gene="5" /locus_tag="SEA_ROSCOWINDEX_5" /codon_start=1 /transl_table=11 /product="major capsid and protease fusion protein" /translation="MTETRESRELHVRAAVNAETRTISGVGVPYGETITIWGERERLA PGSVEAEGCKLFSRHRDPIGIVTEARDDEAGWHPTAKISATPAGDEAYQLARDGVLDG LSIGFDPVEWHVERDDLGDVIVYDRVRVREVSLVPFPAYPSARVESVRELPTNNNPRR EDTPNMNDEQERGDDLREIREQVQDLGRQVALAGQQRAAEPVADERSAGAWLRDLVRG DADAIREYEGMIERAYAGGVAADGILTPQYVGDTIRLIEAPNVLGSIFSTGVLPSKGL QLEYGVLESDTVDVDEQENEGDDLTSGSISLDVDHAEIKTYGGYVELSRQKIERTTNV NVLDLHLRALALRAGRRKAAVLRKHYADLVTANMADPDRVAVVEDATDWIDWLGAIID GAEWFADLGLSLDALVAGKDRFKELASLKDSANRPLMVVSGTGDNTVGRINAKTLGGD LAGVTVRLDLKGAADRAAFVNGEAIRQYNGPVVELADENIVNLSKRFGVYYYAANATE IPAAVVPVVAALPAGVKDGK" gene 5385..5738 /gene="6" /locus_tag="SEA_ROSCOWINDEX_6" CDS 5385..5738 /gene="6" /locus_tag="SEA_ROSCOWINDEX_6" /codon_start=1 /transl_table=11 /product="head-to-tail adaptor" /translation="MPDESQEQTELQQLAEQLRAYVTPEARHVAVDDAFVQRCASEAL ALVGQLIGTTVTVPAEIRQRAIIEAGSELFHKRQAPNGISQFADATGTPLRVAKDPMN VARVILQPFLPLGFA" gene 5735..6115 /gene="7" /locus_tag="SEA_ROSCOWINDEX_7" CDS 5735..6115 /gene="7" /locus_tag="SEA_ROSCOWINDEX_7" /codon_start=1 /transl_table=11 /product="tail terminator" /translation="MSVLGDLRRELADDVAGSPVELTTYDHVPARVQLPAAFVVAGAP YVEADQTFGSSIVRFGVVLLTQPSMNADETDQLDERIESVQRRLLSAGWLVERIERPE IQDLNGAEVLATALSVAAGGVTFP" gene 6154..6588 /gene="8" /locus_tag="SEA_ROSCOWINDEX_8" CDS 6154..6588 /gene="8" /locus_tag="SEA_ROSCOWINDEX_8" /codon_start=1 /transl_table=11 /product="major tail protein" /translation="MGSTRIRGNRKPQLTLGGPGVDRSADVISWTIENEEADADVVTF EDAAEGGGRQFYLRGSAIQSTDTAAFWRYVWENSGEENVPYTIAPHGNAAPSETEPHF VGTLTIGPKPTIGGEASTDPKSAFTFDYEFAIDGEPTMDTGA" gene 6601..6984 /gene="9" /locus_tag="SEA_ROSCOWINDEX_9" CDS 6601..6984 /gene="9" /locus_tag="SEA_ROSCOWINDEX_9" /codon_start=1 /transl_table=11 /product="Hypothetical Protein" /translation="MAEYQNRGIRIDGLRELNAKLRAAGDESADLPDLMFRLGSIVIA NARVPAKSGELAGTLRAGRGRTKAVVRAGYAKRGAHAGVVHYGNPHTGSRAQPFLVDA LRRAQSQLVSELQTGIDQLMRKHKL" gene 6998..7434 /gene="11" /locus_tag="SEA_ROSCOWINDEX_11" CDS join(6998..7283,7283..7434) /gene="11" /locus_tag="SEA_ROSCOWINDEX_11" /codon_start=1 /transl_table=11 /product="tail assembly chaperone" /translation="METSTKKFDLDQLTLGEVAAIEDLSGVAIGSVSESTPQGKFLAA LYMVAKRRDGQPTFTFNAALQASMAEAQSFLGFDAPDAADDADAAESSAEGKRRQLAR ERARLKAQFIVQLGMDPAAYERLTIAERDAIVRELNKRSSRRR" gene 6998..7318 /gene="10" /locus_tag="SEA_ROSCOWINDEX_10" CDS 6998..7318 /gene="10" /locus_tag="SEA_ROSCOWINDEX_10" /codon_start=1 /transl_table=11 /product="tail assembly chaperone" /translation="METSTKKFDLDQLTLGEVAAIEDLSGVAIGSVSESTPQGKFLAA LYMVAKRRDGQPTFTFNAALQASMAEAQSFLGFDAPDAADDADAAESSAEGNGDSSPE NAPA" gene 7553..9658 /gene="12" /locus_tag="SEA_ROSCOWINDEX_12" CDS 7553..9658 /gene="12" /locus_tag="SEA_ROSCOWINDEX_12" /codon_start=1 /transl_table=11 /product="tape measure protein" /translation="MAKNTVIVSILGDTRDLQSKLGGATGSLGKWGAAAAAAGAAVAA AVGVGVAKSVKSASELQQNLGAMDSVFKGNAAQMQQWANGAAGAVGLAKSEYAGLATV LGSQLRNMGVESAALAGKTNELIGLGADLSAQFGGSTSDAVSALSSLLRGERDPIERY GVSINEAAVQAKMAEMGLTGLTGEAEKNAKLQATLALLYQQTADAQGAFARESTTLAG AQQRLAAGAENLFATFGTALLPAFTAVTAAAGVLLNKLQGSDWFASVTASITGASNAF ADFVFGLLNGTSSLDFGSLFAGLLPAVISGVQAAAGWIAGGGLNSLVAGLTAGRGAML DGAVQLFTALAQALPMILPALLTAVLGFITQMIAQLATFVPLLLNAGVQMFTQLITAL VTVIPSILTTLVTLLPGILTTLLGMIPVILSAAVQVFTQLVAAIPVIIPPLIEAVVGL LPQLVASIVSMLPGILQGAIELFTALVQALPIILPTLLTGILNLLPSILSSVISMIPA LINGAVQLFTGIVQALPKIIPQLISALIGLAPTMISTLIGLVPQLIRAGVDLIGGLVK GLMQAAGSVGSALLEIAQNAVGDFLSFLGIHSPSRLFMGFGKNTVQGLVLGLTRNAGL VDGAMGALSSRVGAGFDPVLTTPEIDSAFNSYGAARASSAGAAPVYQISLSTLNATAE TGRVIVEAIRDYEDAGGRL" gene 9655..10680 /gene="13" /locus_tag="SEA_ROSCOWINDEX_13" CDS 9655..10680 /gene="13" /locus_tag="SEA_ROSCOWINDEX_13" /codon_start=1 /transl_table=11 /product="minor tail protein" /translation="MTVIERPLFGRVEIRRLRRNLITAPRGNSATGWQSTVGAGTSPV VTATSTAGMNVGGLGIDTFLRAQATTTGTYVDIRNTGVQGVLVQPGGSVTASAWVRPT TITGAQGFAFIQFYDGAGVVVATATSPAFATPSGSWTRIHVSGVVPAGVVRVRAIFRA TGSVVSGSRLDATAFLVEHAAQLGSWYETTAPGYGELAHGTNLTIRRGGARTGLGLKT DVGLMTFQLLDSEDPMRGGTFQPGQDLRAVSRDLNGQLRELFTGRVVDVAASYPLNKA TGRQRVVTTVTVADAVKTHGETPRYGVAIPTGFETFEARISRLAGSALAPIEAPAEGA PREVYAF" gene 10680..12749 /gene="14" /locus_tag="SEA_ROSCOWINDEX_14" CDS 10680..12749 /gene="14" /locus_tag="SEA_ROSCOWINDEX_14" /codon_start=1 /transl_table=11 /product="minor tail protein" /translation="MPELTRVSKSSPATTFFMGADLLSQDENRNVSIVRCYIGADNGP GGSTGSYFADYGEQQGHMENVVHFLTHAGKPFLPSGVPNGGRRWLDVRDVEVGHGSDG RRGAVSLRMDLKYGPVNESHWGTFNNFPRIAKAPSAPGVSSLSNITTNSVRVNGTAPG DDGGAPILEYQFIAATDVSFSQGIVYSTNAGAIHDVPGLTPGQRYWFAVRARNRRGWS AWSNAGAAAPSAFVGLPAPTFTGWAQNAAGALVATWTAPNPATGLTGYRLQIARDAGF TVGVQNIDVANVLQHAVAGLAGGRRYWARVAARTAGGVNALSAARDALLVLSAGDLDG WTRVGQLPAGMAAFTAEGIRRGTVAGRQALVIENLSTSAAQLTTGQLGLQRVVTGLTI GNAYRFRASAQLTEAGALARQYRLQVVGEGAGPVTAVTTALTALGDGIEFVADTTTAT LQILLAEGVTVPANTEAVERVAFSGIELVELVTDYPVRLRETVYESNLANHFDLACNS VGASWYVGHDGVTRFRLPGAALPVSAVFTDEIDAGALHYIDVAAAYDTRGMVNRLDVT NYGVAEDRETEENSELIVVEQNSIDAFGVRSDRLEVNLWDRAPYDESLTDRLANLLAE SAEPRLFVSSFRWNAQENLPAANALDVGQRITVRFNGTEQDSQIVALQHDITPRRWIV TVTLRRL" gene 12751..13299 /gene="15" /locus_tag="SEA_ROSCOWINDEX_15" CDS 12751..13299 /gene="15" /locus_tag="SEA_ROSCOWINDEX_15" /codon_start=1 /transl_table=11 /product="minor tail protein" /translation="MALRQLEEAVRNLQRRVGEILQQLAPVGTIHAFGGSNAPANYVP LDGRQLSRTEYAALFALIGVTYGPGNGSTTFNVPDARGRALVGIDTAQTEFNVLGKTG GAKTHTLTEAQLPSHTHGVGANADGFAAHRTNTTGGYRAVFASGSTGEVFDYRAPAAA GGGQAHNNLQPYLTVTYVIKAK" gene 13312..13623 /gene="16" /locus_tag="SEA_ROSCOWINDEX_16" CDS 13312..13623 /gene="16" /locus_tag="SEA_ROSCOWINDEX_16" /codon_start=1 /transl_table=11 /product="Hypothetical Protein" /translation="MSHHEGNTMTLIEDVLSEQIAALRASAGLFVRQRADALAAAEAA AVQAAGADARAAALQAELDAYRELLPAPEIPEDAPAVELPADETTPPLYQQLLTEREG N" gene 13642..14337 /gene="17" /locus_tag="SEA_ROSCOWINDEX_17" CDS 13642..14337 /gene="17" /locus_tag="SEA_ROSCOWINDEX_17" /codon_start=1 /transl_table=11 /product="endolysin" /translation="MTMYPHGAKTPPKVSSPFGPRDPRVGISSQHNGADLIGFEDVHA VEAGTVTSAGWMNNAAGITVVIDHGGGITSLYMHLERVSVSRGARVAEGAKIGEVGST GNATGDCLHYEIRLNGRSIEPMGWTAARINAGAPAAAAPAYPLPWGSYFGPKSGPRES VSGYYSHREDLRRWQARMIARGWDLGPAGADGLYGDRTGDVAEAFQREKGLTVDRKIG PATWAAAWTAPIT" gene 14304..14567 /gene="18" /locus_tag="SEA_ROSCOWINDEX_18" CDS 14304..14567 /gene="18" /locus_tag="SEA_ROSCOWINDEX_18" /codon_start=1 /transl_table=11 /product="membrane protein" /translation="MGGSVDGPDHVTATEWTLIAVAVVTASGAWLNARYGRMGRIERR LASVENLNNRLWAYVRQQHDHAYRSGYVPLPIPNHLFENGDPE" gene 14564..14788 /gene="19" /locus_tag="SEA_ROSCOWINDEX_19" CDS 14564..14788 /gene="19" /locus_tag="SEA_ROSCOWINDEX_19" /codon_start=1 /transl_table=11 /product="membrane protein" /translation="MTDPAPLTRRERRQQLRASSERFLTPAFRRWAYGVAGAAVAAAV FAGWLPEGALAVILPLIMAVLYVDKTGAPL" gene complement(14857..15069) /gene="20" /locus_tag="SEA_ROSCOWINDEX_20" CDS complement(14857..15069) /gene="20" /locus_tag="SEA_ROSCOWINDEX_20" /codon_start=1 /transl_table=11 /product="Lsr2-like DNA bridging protein" /translation="MATRTIELLTDDLDGSDGDDVATVTFGLQGVTWEIDLSAENAAQ LDQLLQPYMDAGRRVKRPRRRRSGSL" gene complement(15072..15572) /gene="21" /locus_tag="SEA_ROSCOWINDEX_21" CDS complement(15072..15572) /gene="21" /locus_tag="SEA_ROSCOWINDEX_21" /codon_start=1 /transl_table=11 /product="helix-turn-helix DNA binding domain" /translation="MSMSVSLDMSMSLSHVQEGLTMELIAPEPAKRPATAMISIDAFA AQRKVATRTVRRWLDAGRVPGAVKVGNKWSLPADAIVQDALPGVMDTVKDTSAPVALR AGAELTVAGVLAPLPVMVPLDTAARVLGVSEYALRRNADYFQLQRLGAHGSYVMPKSR IRELEG" gene complement(15653..15883) /gene="22" /locus_tag="SEA_ROSCOWINDEX_22" CDS complement(15653..15883) /gene="22" /locus_tag="SEA_ROSCOWINDEX_22" /codon_start=1 /transl_table=11 /product="helix-turn-helix DNA binding domain" /translation="MTLIQIDSSGLPEILRATRGAAGLSMRDMARQVGVSHGTISAWE RGISEPSVSQFMRWAEVAGQAPERLLPAHTAA" gene 16599..16781 /gene="23" /locus_tag="SEA_ROSCOWINDEX_23" CDS 16599..16781 /gene="23" /locus_tag="SEA_ROSCOWINDEX_23" /codon_start=1 /transl_table=11 /product="Hypothetical Protein" /translation="MAAGSVALGGIVAQLDRLDLGIDRIAAERVDAGDVIAWLEWHSI HLYPYQRRAIEQLLPA" gene 16865..17083 /gene="24" /locus_tag="SEA_ROSCOWINDEX_24" CDS 16865..17083 /gene="24" /locus_tag="SEA_ROSCOWINDEX_24" /codon_start=1 /transl_table=11 /product="helix-turn-helix DNA binding domain" /translation="MPTMTAVATNAPLPTHAKKLTAGQVAELFGVRVETVRRWADAGK IPCTRTLGGDRRFDPAVVQRILHDVAAA" gene 17080..17379 /gene="25" /locus_tag="SEA_ROSCOWINDEX_25" CDS 17080..17379 /gene="25" /locus_tag="SEA_ROSCOWINDEX_25" /codon_start=1 /transl_table=11 /product="HNH endonuclease" /translation="MSQHSSRSAEYKSNRLLCLNRDGWICTYCRCELIEGKNATADHV VSKATWIREGRDGSPDQLDNLVASCTSCNSSKGDRDTMPRVNYYNPRWFAGLVPA" ORIGIN 1 gttggcattt tcaccagatc agagtcagaa atatttgaga atgagcgata cgaccaccac 61 tgacgccgaa ccgttcgagc tggacgccaa ttcgacctgg atcgcagctg tcgagctgtt 121 ccagaaggag ggggccgagt ggctgacggc cgctgacgcg ccgcagctca tggcgctacg 181 ctcgatcgcg aagcagctcg acggcggcac gttccaagcc gccctgatct cgcagttcac 241 cctgatccac cgggcgctgc tcaaccgccg gcctggcgag ccgcagcagc cgaccgatcc 301 cgccggcgca gctcggcagg cagccatggg gccgagcctg ttcgagcagc tgggcggcgt 361 gtggacggcc gacgcatgag ccggcagctg cacctggact caccgcgcta cccgcagccg 421 tgcggggatc gtgaatgccg gcgctgctac ccgccgacgc cggccgaggc gcgagccgcc 481 tacgtcgccg cgtgggacga gctgcagcgc tcggatctcg accgcttccg cgaggccgtc 541 gagcagctgc acgccgccat ggtcgagctg ctgctgcccg tgttcgaggc gttcgcggat 601 ctggtcgagc gcctggcgtg gctgatcgag gatagccggc cggtgcgctg gctgaaccgt 661 cgagcgcgcc gcgccgccat gcgccggcat ccgtcgagcc ggtggcagtt caccccgatc 721 accgccgagg ctggccggca gctcggcctg gcgttcggcc agggcgtcga gatcgcaatg 781 cgccgcgagg cggtcacgtc ggccgaggcg ctggcgctgc gcgccggcat ccgcctgccc 841 gagctgccgc cggcgcggat ctacacgccg ccggctgcgc tgtcgtgggt tgacgttgac 901 cggctggcag agcgcgccgg ccaggatctc ggcacgttcg acgcgatcct ggcagccccg 961 acgccggccg agatcccgcc cgccccgctc tgccagccgg tcaacgccgg ccagcacgtc 1021 ggccggatgc gccggcacac ctggcgcgag ctgctgcagc tgcccggtag ggacatggcc 1081 tagacatgtc catgtcacag ccctaccccg agatcgcgcc atggcccccg aggcgctaca 1141 cgccgcccct gagcgacgat ttcccgagcg cgttcgacgg ctaccgcgat ctgttccggc 1201 tgatctggtt cgcggcgttc ggctacgcgc tcgaagcgtg gcaagagcag gccgtgcgcc 1261 acgtgctaga gctgtacccg gccggccacc gtcgagccgg tcagctgcgc tggcggcagg 1321 ttgtgatcag tctcggccgg cagaacggca agaccgagat agccgcagct atcggcctgt 1381 tcgcgctgct gatgaaagcc gcccccaacg tcgtggggat cgcgtcgagc gccgagcagg 1441 cccgcctggt ctacaagcgc accatgcgcg cgatccgcgg cacgccgaag ctcgcgcagc 1501 gcttccgggc gctcaccgag acccgaggca ttcagaccaa agacggcggg cagtacgaga 1561 tcaaggccgc caagtcggcg gcgctgcagg gcatcccgat cgacgccggc ctggtcgatg 1621 agctgcatat cctcgcgcgc gctctctggt tcgatctggt caacggcatg ggcggccggc 1681 ctaactgttt cgtcgtcggg atcacgaccg ccggcgacga gaacagcgag ctgctgctgc 1741 acctgtacga gcagggatct gaggcgatcg acgccggcgc ggatgcccgg ttcggtttct 1801 acgtctggga ggccgacgcg gccgagatcc ccgacgacga cgacgagctg gggcgcgagc 1861 tggcccgcgc caatccgtcc gtggcatccg gccgcgtgga cctagagaac gctctgatcg 1921 aggttcggtc catgcccaag tcggacgcga tccgctaccg cctgaatcgg ttcgtgcaat 1981 cggtctctga gttcatcacg gccgccatgt ggcacgacaa tttcgtgcgc gaggactggc 2041 ccgagggcgt cgcgcctatc ttcacgatcg accgcacgcc cgactggtcg gctgccacga 2101 tctcggcgtt cggcaagcgc cctgacggcc ggatctactg cgacgtggtg gcgtcgatcg 2161 tgcgcccgga tctcgcccgc ctggtcgagc tgtgcgagca gctgaacgct gcgcaccggc 2221 cggccacgtt cggcatggac agcttcacgc tgcgcgacct cggccgcgag ctggatctgc 2281 gaggcatccc cacgaccatg gccggcatag ccgacgtgat gaacggcagc gcgctgttct 2341 acgcgaagct gcagcagcag cgcctggcgc atccggggca cgatctgctc gcccaccaga 2401 tcccgctcac gaagcggaaa gacagtgacg gcggtttcaa gatcagccgg caggcgtcca 2461 gcgcgtcgat cgacggcgtt atgtcgcacg tgatcggcgt gcagctggcc gagaatcacc 2521 gcgacaacgc cctgcagatg ttctaggaca cgtggacatg actaggacat gtccagtcac 2581 gtgacataaa tcacttacct agcgatgccg tggcaatggc cgcgcgtccg aattaccgcc 2641 cacgtggtca gctgagtaac cattcttcac gtgggacgca tccgagagtg gctggccggc 2701 agtagccgaa gcgcgagcag cgacaacccg acaaccggcg tgacagcgcc gagccggtcg 2761 gccgctctcg ggaccgtgac cgtggccgac gcgttcggga tctcgatggt ctatcgggct 2821 atccagatcc acgcgatcag cgccaagcag ctgagcatcg agaccacgcg ctacggccgc 2881 atggtcgaag atcaccccct gacccgccgg cctgatccgc agtccacgcg cagcagctgg 2941 atcgagcagg ttgtggtgtc catggccagc accggcaacg gttacgccga gatcgtgcgc 3001 gacgcgttcg ggcagcctat cgctctgccg gtgctcaacc cgctgcaggt ccgcattcac 3061 acggatgccg ccggccacgt cacgaagtac agctaccgca accgcgagct gcagccgcgc 3121 gacgtgatgc acgtcacgct gctgcgcgtt cccggctcgg cctacggtct cggccccgtg 3181 caggctgcgc agccggatct gcgcggcgcg atctccaccc gcgactacgc ggctgcctgg 3241 ctggacgaca gcggcgtgcc caccggcgtg ctgaagtcgg atcagaacat cacgcgtgac 3301 acggccgcca tggccaagga gcactggaac gagaacgccg gccagaaaaa cggcgtcgtc 3361 gtgctcgggc agggactcga ctaccggccg atcttcctca gccccaagga tgtccagttc 3421 atcgagtcgc agcagttcag cgtgacgcag atcgcccgcc tgttcggcac gccggcgtcg 3481 ctcatgctgg cggccgtcga gggcaatagc cagagctaca gcaacgtcga gcaggactgg 3541 ctggcatatg tccggttcac gctcatgggc tatctgaccg agatcgagga tgccctgtcc 3601 gagctgctgc ctggtgcgcg tcgcgcccgg ttcaacatcg aggcgctgct gcgcgccgac 3661 accacgacgc gatacgcgag ctacaagacg gccattgagg ccggattcct gaccgtgccc 3721 gaggtccgcg agatcgaggg atgggcaccg ctgcccgacg acgccgagac cccgagccag 3781 gagaccccca ccgcatgact gagacccgag agagccgcga gctgcacgtg cgcgcggccg 3841 tcaacgccga gacccgcacg atcagcggcg tgggcgtgcc ctacggcgag accatcacga 3901 tctggggaga gcgcgagcga ctggcccccg gcagcgtcga ggccgaagga tgcaagctgt 3961 tcagccggca ccgcgacccg atcggcattg tgaccgaggc tcgcgacgat gaggccggct 4021 ggcaccccac ggccaagatc agcgcgacgc cggccggcga tgaggcgtat cagctcgccc 4081 gcgacggcgt gctggacggt ctctcgatcg ggttcgatcc cgtcgagtgg cacgtggagc 4141 gcgacgacct cggcgacgtg atcgtgtacg accgcgtgcg cgtccgtgag gtctccctgg 4201 tccccttccc cgcttacccc tcagcgcgcg tcgagagcgt gcgcgagctg cccaccaaca 4261 acaacccccg tcgagaggac acccccaaca tgaacgatga gcaggagcgc ggcgacgacc 4321 tccgcgagat ccgcgagcag gtgcaggatc tcggccgcca ggtggccctg gctggccagc 4381 agcgtgccgc cgagccggtg gccgacgagc gcagcgccgg cgcgtggctg cgcgacctgg 4441 tgcgcggcga cgccgacgcg atccgcgagt acgagggcat gatcgagcgc gcctatgccg 4501 gtggcgtggc cgctgacggc atcctgacgc cgcagtacgt gggcgacacc atccgcctga 4561 tcgaagcgcc gaacgtgctc ggctcgatct tcagcaccgg cgtgctgccg tccaaggggc 4621 tgcagctcga atacggcgtt ctcgagtccg acaccgtgga cgtggacgag caggagaacg 4681 agggcgacga cctcacctcg ggttcgatct cgctcgacgt ggatcacgcc gagatcaaga 4741 cctacggcgg ttatgtcgag ctgtcccgcc agaagatcga gcgcaccacc aacgtgaacg 4801 tgctcgacct ccacctgcgc gccctcgcgc tgcgcgccgg ccgtcgcaag gctgccgtgc 4861 tgcgcaagca ctacgccgac ctggtgaccg ccaacatggc cgatcccgat cgcgtggccg 4921 tggtcgagga cgcgaccgac tggatcgact ggctgggcgc gatcatcgac ggcgcggaat 4981 ggttcgctga cctcggcctg tcgctcgacg cgctggtggc cggtaaggac cggttcaagg 5041 agctggcgag cctgaaggac agcgccaacc gcccgctgat ggttgtcagc ggcaccggcg 5101 acaacaccgt gggccggatc aacgcgaaga ccctcggcgg cgatctcgcc ggcgtcacgg 5161 ttcgactcga cctgaagggc gcagccgatc gtgccgcgtt cgtcaacggc gaggcgatcc 5221 gccagtacaa cggccccgtg gtcgagctgg cggacgagaa catcgtgaac ctgtcgaagc 5281 ggttcggcgt gtactactac gccgccaacg cgaccgagat cccggccgct gtcgtccccg 5341 tcgtcgcggc cctgccggcc ggcgtcaagg acggcaagta agacatgccc gacgagagcc 5401 aggagcagac cgagctgcag cagctcgctg agcagctgcg cgcctacgtg acgcccgaag 5461 cgcgccacgt ggccgtcgat gacgcgttcg tgcagcgatg cgcgtcagag gctctggctc 5521 tcgtcgggca gctgatcggc accaccgtga ccgtgccggc cgagatccgg cagcgggcga 5581 tcatcgaggc cggcagcgag ctgttccaca agcgccaagc gcccaacggg atctcgcagt 5641 tcgcggatgc caccggcacc ccgctgcgcg tcgccaaaga ccccatgaac gtggcgcgcg 5701 tcatcctgca gcccttcctg ccgctcgggt tcgcatgagc gtgctcgggg atctgcgccg 5761 cgagctggcc gacgacgtgg ccggctcgcc ggtcgagctg acgacctacg accacgtgcc 5821 ggctcgcgtg cagctgccgg cggcgttcgt cgtggccggc gcgccctacg tcgaggcaga 5881 tcagacgttc ggcagctcga tcgtgcggtt cggcgtcgtg ctgctcacgc agccgagcat 5941 gaacgccgac gagaccgatc agctggatga gcggatcgag agcgtgcagc gccggctgct 6001 gagcgccggc tggctggtcg agcggatcga gcgccccgag atccaagacc tgaacggggc 6061 cgaggtactg gccaccgccc tgagcgtcgc cgccggcggc gtgacattcc cctgacatgt 6121 cctagacagt ccacccaaga gagagagacc cccatgggca gtacccgtat tcgcggcaac 6181 cgcaagccgc agctgacgct cggcggcccc ggcgttgacc gttcggccga cgtgatcagc 6241 tggacgatcg agaacgaaga ggccgacgcc gacgtggtga cgttcgagga cgcggccgag 6301 ggcgggggcc ggcagttcta cctgcgcggg tccgcgatcc agtccaccga cacggccgca 6361 ttctggcgct acgtctggga gaacagcggc gaggagaacg tgccctacac gatcgccccg 6421 cacggcaacg ctgcgccgtc cgagaccgaa ccgcatttcg tcggcacgct gacgatcggc 6481 cccaagccga ccattggcgg cgaggcgtcc accgatccga agtcggcgtt cacgttcgat 6541 tacgagttcg cgatcgacgg cgagccgacc atggacaccg gcgcgtaagc ggggcggcgc 6601 gtggctgagt accagaaccg cggcatccgt atcgacgggc tgcgagagct gaacgcgaag 6661 ctgcgcgccg ccggcgacga gagcgccgac ctgccggatc tcatgttccg gctcggctcg 6721 atcgtcatag ccaacgcgcg cgtgccggcc aagagcggcg agctggccgg cacgctgcgc 6781 gccggccgag gccgcaccaa agccgtggtg cgtgccggct acgccaagcg tggagcacac 6841 gccggcgtcg tccactacgg caacccccac acgggcagcc gagcgcagcc gttcctggtg 6901 gacgcgctgc gccgtgccca atcccagctg gtcagcgagc tgcagaccgg catcgaccaa 6961 ctgatgcgta agcacaagct ctagaaaggg cacccacatg gaaacctcca cgaagaaatt 7021 cgacctggat cagctcaccc tcggagaagt ggcagcgatc gaggatctgt ccggcgtcgc 7081 gatcggcagc gtgtccgaga gcaccccgca gggcaagttc ctggcggcgc tctacatggt 7141 ggccaagcgc cgcgacgggc agccgacgtt cacgttcaac gcggcgctgc aggcgtccat 7201 ggccgaggcg cagagcttcc tcgggttcga cgctcccgac gccgccgacg acgccgacgc 7261 ggccgagagc agcgccgagg gaaacggcga cagctcgccc gagaacgcgc ccgcctaaag 7321 gcgcagttca tcgtgcagct gggcatggac ccggccgcgt atgaacgcct gacgatcgcg 7381 gagcgcgacg cgatcgtgcg cgagctgaac aagcgcagca gccggcgcag atagccggcg 7441 gacatgtcct agacatgtca ccctgaccgg ccggccccga caaccccccc agctacccca 7501 gcacgtcggg gccggccccc agatttcccc ccgagaggac actagcgccg ccatggccaa 7561 gaacaccgta atcgtgtcga tcctcgggga tacccgcgac ctgcagagca agctcggcgg 7621 ggccacggga tcgctcggca agtggggcgc agctgcagct gcagccggcg cggccgtcgc 7681 tgccgctgtc ggcgtcggcg tcgccaagag cgtcaagagc gccagcgagc tgcagcagaa 7741 cctcggggcc atggacagcg tgttcaaggg caacgccgcg cagatgcagc agtgggcgaa 7801 cggcgcggcc ggcgcggtcg gcctggctaa gtccgagtac gccggcctag cgaccgtgct 7861 cggctcgcag ctgcgcaaca tgggcgtgga gagcgccgcg ctggccggca agactaacga 7921 gctgatcgga ctcggcgcgg atctctcggc gcagttcggc ggcagcacgt cggatgccgt 7981 cagcgccctg tcgtcgctgc tgcgaggcga gcgagacccg atcgagcgct acggcgtgtc 8041 gatcaacgaa gcggccgtgc aggcgaagat ggccgagatg gggctaaccg gcctcaccgg 8101 cgaggccgag aagaacgcga agctgcaggc caccctcgcg ctgctgtatc agcagaccgc 8161 cgacgcgcag ggcgcattcg cccgcgagag caccacgctg gccggcgcgc agcagcgcct 8221 ggctgccggc gcggaaaacc tgttcgccac gttcggcacg gcgctgctgc cggcgttcac 8281 ggccgtgacc gcagcggccg gcgtgctgct caacaagctg cagggcagcg actggttcgc 8341 cagcgtcacg gcgtcgatca ccggcgcgtc taacgcgttc gcagatttcg tgttcggcct 8401 gctcaatggc acgtcgtcgc tcgatttcgg ctcgctgttc gccggcctgc tgccggccgt 8461 gatctcgggc gtgcaggctg cagccggctg gatcgccggc ggcggtctca acagcctggt 8521 ggccggtctg acggccggcc gtggggccat gctcgacggg gccgtgcagc tgttcacggc 8581 gctggcgcag gcgctgccga tgatcctgcc ggcgctgctc acggccgtgc tcgggttcat 8641 cacgcagatg atcgcgcagc tggccacgtt cgtgccgctg ctgctcaacg ccggcgtgca 8701 gatgttcacg cagctgatca ccgctctggt gaccgtcatc ccgtcgatcc tcaccacgct 8761 ggtgacgctg ctccccggca tcctcaccac gctgctgggc atgatccccg tcattctcag 8821 cgcggccgtg caggtattca cgcagctggt ggccgctatc cccgtgatca tccccccgct 8881 gatcgaggcc gtggtggggc tgctgccgca gctggtggcg tcgatcgtgt ccatgctccc 8941 cggcatcctg cagggcgcta tcgagctgtt cacggcgctg gtgcaggcgc tgccgatcat 9001 cctgcccacg ctgctgacgg gcattctcaa cctgctgccg tcgatcctgt catccgtgat 9061 ctctatgatc ccggcgctga tcaacggggc cgtgcagctg ttcaccggca tcgtgcaggc 9121 gctgcccaag atcatcccgc agctgatcag cgcgctgatc ggtctcgcgc ccaccatgat 9181 ctcgaccctg atcggtctcg tgccgcagct catccgcgcc ggcgtcgatc tgatcggcgg 9241 tctggtcaag ggtctgatgc aggcggccgg atcggtcggc agcgcgctgc tcgaaatcgc 9301 gcagaacgcc gtaggggact tcctgagctt cctcggcatt cactcgccta gccggctgtt 9361 catggggttc ggtaagaaca ccgtgcaggg tctggtgctc ggcctgacgc gtaacgccgg 9421 cctggtcgac ggggccatgg gcgcgctgag ctcgcgcgtg ggggccgggt tcgatcccgt 9481 gctgaccacg cccgagattg acagcgcatt caacagctac ggcgccgcgc gcgccagctc 9541 ggccggcgcc gcgccggtct accagatcag cctcagcacg ctgaacgcga cggccgagac 9601 cggccgcgta atcgtcgagg cgatccgcga ctacgaggat gcggggggcc ggctgtgacc 9661 gtcatcgagc gcccgctgtt cggccgcgtc gagatccgcc ggctgcgccg taacctgatc 9721 accgccccgc gcggcaactc ggctaccggc tggcagtcca ccgtgggggc cggcacgtcg 9781 cctgtcgtga cggcgacgag caccgccggc atgaacgtgg gcggtctcgg gatcgacacg 9841 ttcctgcgcg cgcaggccac caccacgggc acctacgtgg acattcgcaa cacgggcgtg 9901 cagggcgtgc tggtgcagcc tggcgggtct gtgacggcat ccgcatgggt tcggccgacc 9961 acgatcaccg gggcgcaggg gttcgcgttc atccagttct acgacggggc cggcgtcgtc 10021 gtggccacgg ccacgtcgcc ggcgttcgcg acgccctcgg gcagctggac ccgcattcac 10081 gtgtcgggcg tcgtgccggc cggcgtcgtg cgcgtgcgtg cgatcttccg cgccacgggc 10141 agcgtggtga gcggctcccg gctcgacgct acggcgttcc tggtcgagca cgccgcgcag 10201 ctcggcagct ggtacgagac caccgcgccc ggttacggcg agctggcgca cggcaccaac 10261 ctgacgatcc gacgcggggg cgctcgcacc gggctgggcc tgaaaaccga cgtcgggctg 10321 atgaccttcc agctgctcga ttccgaagac cccatgcgcg ggggcacgtt ccagccgggg 10381 caggatctgc gcgcggtctc tcgggatctc aacggccagc tgcgcgagct gttcaccggc 10441 cgcgtggtcg atgttgccgc cagctacccg ctcaacaaag ccaccggccg gcagcgcgtc 10501 gtcaccaccg tgaccgtggc ggatgccgtc aagacccacg gcgagacccc gcgctacggc 10561 gtggcgatcc ctaccgggtt cgagacgttc gaggcgcgca ttagccggct cgccggctcg 10621 gcactggccc cgatcgaagc gcccgccgag ggcgcaccta gagaggtcta cgcgttctaa 10681 tgcccgagct gacccgagta tctaagagca gccccgcgac cacgtttttt atgggcgcgg 10741 atctgctgag ccaggacgag aaccggaacg tttcgatcgt ccgctgctat atcggcgctg 10801 acaacggccc cggcggcagc accggttcgt atttcgctga ctacggcgag cagcagggcc 10861 acatggaaaa cgtggttcac ttcctgaccc acgccggaaa gccgttcctg ccctcgggcg 10921 tgcccaatgg cggccggcgc tggctcgacg tgcgcgacgt ggaggtgggc cacggcagcg 10981 acggccgacg cggcgctgtc agcctgcgca tggatctgaa atacggtccc gtcaatgaga 11041 gccactgggg cacgttcaac aacttccccc ggatcgccaa agcgccgagc gcgcccggtg 11101 tcagctcgct gagcaacatc accaccaaca gcgtgcgcgt gaacggcacc gcgcccggtg 11161 acgacggcgg cgcgccgatt ctcgagtacc agttcattgc cgctacggat gtcagtttct 11221 cgcagggcat cgtctattcg accaacgccg gcgcgatcca cgacgtgccc ggactcaccc 11281 ccggtcagcg ctactggttc gctgtccgcg cccgcaaccg gcgcggctgg tccgcgtggt 11341 ctaacgccgg cgcagcggcc ccgagcgcgt tcgtggggct gccggccccg acgttcaccg 11401 gatgggcgca gaacgccgcc ggcgcgctgg tggccacctg gaccgccccc aaccctgcca 11461 ccggcctgac cggctaccgg ctgcagatcg cgcgggatgc cgggttcacg gtcggcgtgc 11521 agaacatcga cgttgccaac gtgctgcagc acgccgtggc cggtctcgcc ggcggccggc 11581 gctactgggc gcgcgtggca gctcgcacgg ccggcggcgt caacgcgctg tcagcagctc 11641 gcgacgcgct gctggtactc agcgccggcg atctcgacgg atggacccgc gtgggccagc 11701 tgccggccgg catggcggca ttcacggccg agggcatccg ccggggcacc gtcgccggcc 11761 ggcaggcgct ggtgatcgag aacctgtcca cgtcggccgc gcagctgacc acggggcagc 11821 tcggcctgca gcgcgtcgtc accgggctga cgatcggcaa cgcctaccgc ttccgtgcat 11881 ccgcgcagct gaccgaggcc ggcgctctgg cgcgccagta tcgcctgcag gtagtggggg 11941 agggggcagg gccggttacg gccgtcacaa cggcgctgac ggccctgggg gacggtatcg 12001 agttcgtggc cgacaccacg accgcgacgc tgcagatcct gctcgctgag ggcgtcaccg 12061 tgccggccaa cactgaggct gtcgagcgcg tcgcattctc gggtatcgag ctggtcgagc 12121 tggtgaccga ttacccggtg cgcctgcgcg aaacggtcta cgagtccaat ctggccaacc 12181 actttgacct cgcgtgcaac agcgtggggg cgagctggta cgtgggccac gacggcgtaa 12241 cccggttccg gctgcctggc gctgcgctgc cggtctcggc cgtgttcaca gacgagatcg 12301 acgccggcgc gctgcactac atcgacgtgg cggccgcgta tgacacgcgg ggcatggtca 12361 accgcctgga cgtgaccaac tacggcgtgg ccgaggaccg cgagaccgag gaaaacagcg 12421 agctgatcgt ggtcgagcag aacagcatcg acgcattcgg cgtgcgctcg gatcggctcg 12481 aagtgaacct atgggaccgc gcgccctatg acgaatcgct gaccgatcgg ctggccaacc 12541 tgctggccga gtcggcagag ccgcgcctgt tcgtgtcgag cttccggtgg aacgcgcagg 12601 agaacctgcc ggcagccaac gcgctggacg tggggcagcg catcaccgtg cgattcaacg 12661 gcacggagca ggattcccag atcgtcgcgc tgcagcacga cattacgccg cgccggtgga 12721 tcgtcaccgt gacgctgagg aggctctgac atggccctga gacagctcga agaagcagtg 12781 cgcaacctgc agcgccgcgt gggcgagatc ctgcagcagc tggcccccgt gggcacgatc 12841 cacgcgttcg gcggcagcaa cgcgccggcc aactacgtgc ccctggacgg ccggcagctc 12901 tcgcgcaccg agtacgccgc cctgttcgcc ctgatcggcg tcacctacgg ccccggcaac 12961 ggcagcacca cgttcaacgt gcccgacgca cgcgggcgcg ccctggtggg catcgacacc 13021 gcgcagaccg agttcaacgt gctcggcaag accggcgggg ccaagactca cacgctgacc 13081 gaggcgcagc tgccgagcca cacccacggc gtaggggcca acgctgacgg gttcgcagcg 13141 caccggacca acacgaccgg cgggtatcgc gccgtgttcg cgtcgggatc gaccggcgag 13201 gtattcgact accgcgcgcc ggccgctgcc ggcggggggc aggcgcacaa caacctgcag 13261 ccctatctga ccgtcacata cgtaatcaag gccaagtgac ctgtctagga catgtcacac 13321 cacgaaggga acaccatgac actgatcgag gacgtgctga gcgagcagat agctgccctg 13381 cgcgcgtcgg ccggcctgtt cgtgcgccag cgcgccgacg ccctcgcagc ggccgaggct 13441 gcagccgtgc aggctgccgg cgcggatgcc cgagccgctg cgctgcaggc cgagctggac 13501 gcctaccgcg agctgctgcc ggcccccgag atccccgagg acgcgccggc ggtcgagctg 13561 ccggccgacg agaccacgcc cccgctctat cagcagctgc tgacagagcg tgagggcaac 13621 tgagagatag gagacagacc catgaccatg tacccccacg gggccaagac gcccccgaag 13681 gtgtcgagtc cgttcggccc ccgcgatccc cgcgtgggga tcagctcgca gcacaacggc 13741 gctgacctga tcgggttcga ggacgttcac gccgtcgagg ccggcacggt cacctcggcc 13801 ggctggatga acaacgccgc cggcatcacc gtggtgatcg accacggcgg cggcatcacg 13861 tcgctctaca tgcacctgga acgcgtcagt gtcagccgag gcgcacgcgt ggccgagggc 13921 gcgaagatcg gcgaggtcgg cagcaccggc aacgcgaccg gggactgcct gcactacgag 13981 atccgcctga acggccgcag catcgagccg atgggctgga ccgctgcccg gatcaacgcc 14041 ggcgcgccgg ctgcagctgc gcccgcctac ccgctgccgt ggggttcgta tttcggcccc 14101 aagtccggcc cccgcgagag cgtcagcggg tactactcgc accgcgagga tctgcgccgc 14161 tggcaggcgc gcatgatcgc gcggggctgg gatctcggcc ccgccggcgc ggatggtctc 14221 tacggggacc gcaccggcga cgtggccgag gcattccagc gcgagaaggg tctgaccgtc 14281 gatcgcaaga tcggccccgc cacgtgggcg gcagcgtgga cggccccgat cacgtgaccg 14341 cgaccgagtg gactctgatc gccgtcgccg tcgtcacggc atccggtgcg tggctgaacg 14401 cccgctacgg gcgcatggga cggatcgagc gccggctggc cagcgtcgag aacctgaaca 14461 accggctttg ggcctacgtg cgccagcagc acgatcacgc gtaccgcagc gggtacgtgc 14521 ccctgccgat ccctaaccac ctgttcgaga acggagaccc cgaatgaccg atcccgcccc 14581 cctgacgcgt cgagagcgcc ggcagcagct gcgcgccagc tctgagcgct tcctgacgcc 14641 ggcattccgc cggtgggcct acggcgtcgc cggcgcggcc gtcgcggctg ccgtgttcgc 14701 cggctggctg cccgagggcg ctctggccgt catcctgccg ctgatcatgg ccgtgctcta 14761 cgtggacaag accggcgcgc cgctctgaga gccgctgaga gcacgaaaca gcgccccctg 14821 cctatacggc ggggggcgct gtcgtgttcg gcgctctcag aggctcccag agcgccggcg 14881 gcgtggtcgc ttcacgcgcc ggccggcatc catgtacggc tgcagcagct ggtcgagctg 14941 cgcggcattc tcggcggata ggtcgatctc ccacgtgacg ccctgcagcc cgaacgtgac 15001 cgtagccacg tcgtcgccgt cgctgccgtc gagatcgtca gtcagcagct cgatcgtgcg 15061 cgttgccatg gtcagccctc cagctctcgg atccgcgatt tcggcatgac gtagctcccg 15121 tgcgcgccga ggcgctgcag ctggaagtag tcggcgttac ggcgcagcgc gtactcgctg 15181 acgccgagca cacgcgcggc cgtgtcgagc ggcaccatga ccggcagcgg ggccagcacg 15241 ccggccacgg tcagctcggc acctgctcgc agcgccacgg gggctgacgt gtctttgaca 15301 gtgtccatga caccgggcag ggcatcctgc acgatcgcgt cggccggcag cgaccactta 15361 ttgccgacct tcacggcacc gggcacgcgg ccggcgtcga gccagcgccg caccgtgcgc 15421 gtggccacct tccgctgcgc tgcgaacgcg tcaatcgaga tcatggcggt ggccggccgc 15481 ttagccggct cgggggcgat cagttccatg gtgagacctt cctggacatg tgacagtgac 15541 atggacatgt ctagtgacac tgacatggac atgtcaagtg acacgcgggc atgacaaagc 15601 gccccacgtc gccggctcgg ggcgatcgtg gggcgcgagc tgctggcaag cctcacgcag 15661 cagtgtgcgc gggcagcagc cgctcggggg cctgtcccgc gacctcagcc caccgcatga 15721 actgcgacac gctcggctcg gagatcccgc gttcccacgc cgagatcgtg ccatgactca 15781 cgccgacctg acgcgccatg tcgcgcatag agagacccgc agcgccacgc gtggcccgca 15841 ggatctccgg cagcccgctg ctgtcgatct gaataagtgt catgtctgtg agtgtatcgc 15901 taggtatcga catatatgcc gacacaccgc aaggaatgcc agcgactcgc tacatgtgcg 15961 taaagactca ccacgtgtct agaatcccaa ccatgcgacc acgaccacca ccaagcgccc 16021 cggcgagggg cgataaccga atacgtggac cggtgagggt ccgacaacgc tgtaggcgac 16081 agcgaaacaa gaacgggtaa tcagtcccgg tgccagcccg agagctaggg gcgcagcggt 16141 caaggcatga ccgggcgctg cagtggcccc caatccatcc ccctagctca tatcccgtcc 16201 gtgacgcgct aaccccgcgt gccgtggtga tctccctagg ctccgcctgg ctccgtcgag 16261 cagatcccat gtgcccctcc cgagttcaga aatgagctag ggggggccat agttcccaac 16321 cccctcacct accttcacca tcgagcaggg cgaagggaca taccccacaa accccacacc 16381 ccgagagcat cacagtgacc aacccgagag agatcgcagc agctgctaac aacctgtcag 16441 agctgacaga acagacccga gcagtagcca cccgagcacg acgagcacga cagatccgac 16501 tgctgaggca agcccggtcc ctaggtgaac aactcgacgc agctcgcact cagctgatcg 16561 ttaccggacc gcaagccctc gcagctgccg aggcgttcgt ggcggcgggt agcgttgctc 16621 tggggggcat cgtcgcgcag ctcgaccgct tggatctcgg catcgaccgg atcgcggccg 16681 agcgcgttga cgccggcgac gtgatcgcgt ggctggaatg gcactccatc cacctgtacc 16741 cgtatcaacg tcgagcgatc gagcagctgc tgcctgcctg accgctctcg acgcgcgccc 16801 gaacgcgtca cctaggcatc ccccacaatc cccacacccc gcataacccg agagagccac 16861 cccagtgccc acgatgaccg ctgtagccac caacgccccg ctgcctaccc acgccaagaa 16921 gctgaccgcc ggccaggtgg ccgagctgtt cggcgtccga gtcgagaccg tccgccgatg 16981 ggccgacgcc ggcaagatcc cctgcacgcg cacgctcggc ggggatcgcc ggttcgatcc 17041 ggccgtggtg cagcgcatcc tgcacgacgt ggcagcagca tgagccagca cagcagccgg 17101 tcggctgagt acaagagcaa ccggctgctg tgcctgaacc gtgacggctg gatctgcacc 17161 tactgccggt gcgagctgat cgagggcaag aacgcgacag ctgatcacgt ggtgtccaag 17221 gctacgtgga tcagagaggg acgcgacggc agccccgatc agctcgacaa cctggtggcc 17281 agctgcacgt catgcaacag ctcgaagggt gaccgcgaca ccatgccacg cgtgaactac 17341 tacaacccgc gctggttcgc cggtctcgtg ccggcctgac cggctcgccc tgcagctcga 17401 cgccgacgcc gagccggttt ctctgtagcg agagccaccg acaccccgcc cca //