Mycobacterium phage Adam007
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Detailed Information for Phage Adam007
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByAzqa Qureshi
Year Found2017
Location FoundDahlonega, GA USA
Finding InstitutionUniversity of North Georgia
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates34.5291 N, 83.9881 W Map
Discovery NotesThe soil where I got the sample was covered in wood chips, so I had to dig a little ways down to reach the moist soil. I got a few small twigs and little pebbles in the sample itself. The sample was granular and dark brown in color.
Naming NotesAt first, I wanted to name my phage “Adam”, like Adam from the Bible, since it was “the first phage” I found. This name was already taken, though, so I put “007” at the end of it because I am a huge James Bond fan.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesThe plaque from my phage are fairly uniform in size; however, there are a few that were larger than others. This is a tell-tale sign that I have different kinds of bacteriophage on my plate. My average plaque size is 0.5 mm. My phage has a high adsorption rate, since I have many plaques on each plate. Although the incubation rate affects the size of the plaque significantly, there are some outside variables like agar that I personally have very little control over. This could affect the number of plaques. For example, the moisture on the agar plates may have decreased the number of plaques I could have obtained had the collected water droplets not have coated the agar/bacteria/phage sample/7H9. The plaques are very clear and there is almost no turbidity.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 5x10e9
Pitt Freezer Box# 74
Pitt Freezer Box Grid# F12
Available Files
Plaque PictureDownload
EM PictureDownload