Mycobacterium phage BigHalo2
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| Detailed Information for Phage BigHalo2 | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Tiffany M Moore |
| Year Found | 2013 |
| Location Found | Columbus, OH USA |
| Finding Institution | The Ohio State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | Unavailable |
| Discovery Notes | The specimen was collected in the morning after a rain at approximately 63 degrees Fahrenheit in an area with high foot traffic in the soil beneath wood chips aside an apartment. |
| Naming Notes | The name was made to represent it's morphology. The original morphology had a <.1 mm clearing surrounded by a less transparent clearing that measured on average ~2.0 mm. It was named a halo because it was not obviously viable unless raised up to the light and presumable a temperate phage. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The burst size was as follow in the naming notes, however the morphology changed dramatically and frequently once the MTL was made. The population was definitively pure before the MTL. So either contamination of another phage or second unstable morphology may be the reason for the inconsistency. It's final morphology does not resemble the original morphology at all. With a burst size of 1 mm and not signs of temperate behavior. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 5.6 x 10^9 |
| Date of SEA Lysate Titering | Nov 15, 2013 |
| Pitt Freezer Box# | 3 |
| Pitt Freezer Box Grid# | D1 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
