Mycobacterium phage BlueHole
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Detailed Information for Phage BlueHole
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByHunter Smith
Year Found2022
Location FoundBowling Green, KY United States
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates36.94971 N, 86.46664 W Map
Discovery NotesI obtained my sample from Blue Hole 1 at Lost RIver Cave, Bowling Green, Kentucky. My sample was very muddy because it was a few inches away from the pond of water. It was very smooth and had a reddish brown color. This mud had almost no debris.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesI have a clear plaques that create small circular rings or halos around the plaques. These rings are only observable when the plaque is isolated on the plate. When not given enough room the phenotype of the phage is not expressed. The typical size of each plaque is 2 mm. When crowded each plaque is forced to shrink.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.2 * 10^8
Pitt Freezer Box# 157
Pitt Freezer Box Grid# F3
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload