| Detailed Information for Phage Bolt007 |
| Discovery Information |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | Kelley Krug |
| Year Found | 2021 |
| Location Found | Malibu, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.047777 N, 118.578333 W Map |
| Discovery Notes | Soil setting:
Urban, couple trees, a cactus, shrubs and brush, 40 ft from a mountainous road, sloped, northeast-facing
Depth from which sample was taken: 12cm
Air temperature: 30
AL Quarter and Lab section: F21-1B
Instructor: Amanda Freise; Ana Garcia Vedrenne
Teaching Assistant: Ryan Ngo
Undergraduate Assistant(s): Amanda Chai
Team #1 Name: Phage on the Run
Team #1 Members: Minehli Kamarzar, Kelley Krug, Maysaa Batteikh, Manar Abuwarda |
| Naming Notes | Our team name was Phage on the Run so we decided to name our phage Bolt007 after the movie characters Bolt and James Bond, since they’re both spies on the run. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 4, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2108 |
| Genome length (bp) | 49798 |
| Character of genome ends | Circularly Permuted |
| GC Content | 69.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | FH |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members |
|
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | In GenBank |
| Plaque Notes | Halo plaques: clear with turbid ring on outside
~1mm on average |
| Morphotype | Myoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OP985600 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
139 |
| Pitt Freezer Box Grid# |
F8 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
