Mycobacterium phage Bombola
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| Detailed Information for Phage Bombola | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Tonisha Pope |
| Year Found | 2013 |
| Location Found | Columbus, OH USA |
| Finding Institution | The Ohio State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | Unavailable |
| Discovery Notes | Discovery Result's During each Procedure. Isolated Novel Phage: Direct Plating From the six samples taken no plaques appeared in the samples. Procedure had to be done over again to see if plaques would appear on plates. Also there was contamination, which was another factor in the procedure being done over again. Purified Phage: Spot Test Checked plates after being incubated overnight at 37 degrees Celsius and, discovered contamination with possible plaques. Due, to the high concentration of contamination within the plates this led to the procedure being done over again. Purified Phage: Plaque Streaking Protocol Plaques were contaminated and had to do procedure over again. Procedure was done three additional times in the plaque streaking experiment due to constant contamination. I finally achieved phage populations on plaques after the third round of streaking that I could isolate and purify to be able to move onto the next step during the next three additional rounds. Phage-Titer Assay Plates were initially contaminated. I did the procedure over again on October 18, 2013. Purified Phage: Final Plaque Purification For my MTL procedure my calculations were 3.2*10^6 pfu/ml. Appearance of the plaques are medium sized with no sign of contamination within the plates. Phage Stocks: Empirical Testing Web pattern is clear to proceed onto 10-plate phage infection.-3 web plate that was previously flooded with PB has enough solution and, a high enough concentration to proceed to get HTL solution from. 10-Plate Phage Infection and Harvest For the ten plate infection for the high-titer lysate I used -3 web-plates for my dilutions. My calculated titer for this procedure equated out to be 3.5*10^9. My concentration is high enough to move onto the isolation protocol. DNA Isolation My results for DNA isolation are that the concentration of my DNA equated out to be 67.1pfu/ml. Digest There is DNA present on my phage genomic DNA photograph but the bands somewhat did not separate and appear smeared. |
| Naming Notes | Phage Designator Name:2013OHSUBombolaTPope I choose Bombola for the name because it's a unique and interesting name. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The morphology of my phage was 1 mm. The size of my plaques were medium and fairly consistent throughout the entire exsperiment. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 3.5*10^9 |
| Date of SEA Lysate Titering | Oct 18, 2013 |
| Pitt Freezer Box# | 3 |
| Pitt Freezer Box Grid# | D4 |