| Discovery Information |
| Isolation Host | Streptomyces griseus ATCC 10137 |
| Found By | Brendon Williams |
| Year Found | 2018 |
| Location Found | Flower Mound, TX USA |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 33.041985 N, 97.172043 W Map |
| Discovery Notes | The soil sample was taken August 26th, 2018, at 3:55PM. The soil was largely dry with organic matter such as grass roots and other buried materials such as twigs and acorns, with very few rocks. The air temperature was 96 degrees F. The sample was taken from about 2 feet away from a tree, and consisted of dark and clumpy soil. |
| Naming Notes | The name Bordeaux comes from the street it was taken from, and also where I grew up. It reflects the temperate nature of the phage as just like the French wine town the street shares its name with, the phage took its time to reach a viable titer. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 8, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 700 |
| Genome length (bp) | 131317 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 12579 bp |
| GC Content | 49.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BE |
| Subcluster | BE2 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | A spot test testing for lysogens had clear spots down to the 10^-7 dilution, lending evidence to a possibly negative conclusion. |
| Other Cluster Members |
|
| Annotating Institution | University of North Texas |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques were typically very small and cloudy. They were typically an average of about 2 mm across. |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN369757 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
4•10^7 |
| Pitt Freezer Box# |
75 |
| Pitt Freezer Box Grid# |
A9 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
