Microbacterium phage Bugger
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Detailed Information for Phage Bugger
Discovery Information
Isolation HostMicrobacterium paraoxydans NRRL B-14843
Found ByTahmara Hendrickson
Year Found2021
Location FoundViroqua, WI USA
Finding InstitutionUniversity of Wisconsin-River Falls
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates43.494722 N, 90.863889 W Map
Discovery NotesSoil Info: mix of wet/dry soil, near my dog's potty place. No roots, minimal grass.

Weather conditions: cool, misty/slightly wet, low humidity.

Animals nearby: my dog and many cats.
Naming NotesThe origin of the name Bugger is that he was my old barn cat that I rescued from under the lawnmower as a kitten at the vet clinic I used to work at. He was my favorite cat for a long time. He was hit by a car a little over a year after I brought him home.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 15, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage638
Genome length (bp)61511
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length1507 bp
GC Content58.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterGB
Subcluster--
Cluster Life CycleUnknown
Other Cluster Members
Annotating InstitutionUniversity of Wisconsin-River Falls
Annotation StatusBeing Annotated (Expected completion by 5/1/2023)
Plaque NotesMy plaques are relatively large compared to others in my class. As the rounds of purification and dilutions progressed, they became more uniform in size, but the photo I am using has multiple variations in size. They remained clear the whole time, and sometimes had a halo, sometimes did not.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

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Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 140
Pitt Freezer Box Grid# B7
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload