Mycobacterium phage BusterBird12
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| Detailed Information for Phage BusterBird12 | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Joseph Crafton |
| Year Found | 2012 |
| Location Found | Spottsville, KY USA |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 37.85595 N, 87.436142 W Map |
| Discovery Notes | The soil sample that my phage was isolated from was collected after a few days of steady rain. The soil was taken from an area that was less than one centimeter below ground level. The soil was dense and wet. Along with small stones, the soil contained noticeable amounts of particulate matter like grass and small parts of leaves. |
| Naming Notes | I named this phage after my deceased dog of 14 years. His name was "Buster". The "Bird" part of the names comes from one of my Henderson County High School principals. Finally, the "12" part of the name comes from the year that my most recent dog was born in. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | At the beginning of isolation and purification, the plaques contained a very small clear center with turbid rings. The center was usually around 2 mm in diameter, and the rind were anywhere from 3-5 mm in width (measured from the edge of the clear part to the outermost part of the plaque). As I continued with isolation, the plaque morphologies began to solidly turbid plaques. Then--this happened within a two day span--I noticed that my phage was returning two types of morphologies. One was completely turbid. The other was completely clear. I do not know what causes these rapid changes. The turbid plaques return turbid and clear plaques. The clear plaques only return clear plaques. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 5.0 * 10^10 pfu/mL |
| Date of SEA Lysate Titering | Oct 30, 2012 |
| Pitt Freezer Box# | 43 |
| Pitt Freezer Box Grid# | 3 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |