Corynebacterium phage C3PO
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Detailed Information for Phage C3PO
Discovery Information
Isolation HostCorynebacterium vitaeruminis NCIB 9291
Found ByBrittaney Johnson and Darshan Patel
Year Found2015
Location FoundBirmingham, AL USA
Finding InstitutionUniversity of Alabama at Birmingham
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates33.44192024533113 N, 86.8248219 W Map
Discovery NotesOriginal sample was sewage influent from a Jefferson County sewage treatment plant. Sample was first filtered through a 0.2uM filter in a biosafety cabinet, then enriched for 48 hours, 30C, 225rpm. Sample was then filtered again through 0.2uM filter in a biosafety cabinet. Filtered enrichment sample then spotted for presence of phage infecting Corynebacterium.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 5, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1180
Genome length (bp)67383
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCCTACGGTGC
GC Content52.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEN
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesPlaques not visible at 24 hour incubation at 30C. Plaques visible at 48 hour incubation. Plaque size variable (1-2mm in diamater).
Number of Genes93
Number of tRNAs4
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMG198776
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.0x10^9
Pitt Freezer Box# 26
Pitt Freezer Box Grid# D9
Available Files
Final DNAMaster FileDownload
GenBank File for PhameratorDownload