Mycobacterium phage Cage
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| Detailed Information for Phage Cage | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Edward Clem Williams |
| Year Found | 2013 |
| Location Found | Providence, RI USA |
| Finding Institution | Brown University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 41.829 N, 71.402 W Map |
| Discovery Notes | The soil containing this phage was collected on 9/6/2013, on Brown University's Pembroke Campus. |
| Naming Notes | The original name for this phage was "Nicolas Phage," reflecting its ability to make a good gel better and a bad gel worse (referencing the common rule towards Nicolas Cage and movies). However, this name did not fit the naming guidelines set out by the HHMI. So, the investigator who discovered the phage had to improvise. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The plaques were not completely clear, and possessed "halos," indicating that the phage most likely reproduced via lysogenesis. As well, at higher titers, the phage displayed "second death" ghost plaques. Fainter plaques appeared scattered across the plate, even though the plate was clearly a web plate. Further purification ensured that the lysate was not contaminated, so it was likely that the phage was killing bacteria early on in the infection process, allowing the plaques to be overgrown and killed again. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 2.1x10^9 |
| Pitt Freezer Box# | 4 |
| Pitt Freezer Box Grid# | C12 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |