Mycobacterium phage Cally
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| Detailed Information for Phage Cally | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Emily Kain |
| Year Found | 2014 |
| Location Found | Indianapolis, IN USA |
| Finding Institution | Hope College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 39.930247 N, 85.972811 W Map |
| Discovery Notes | Cally was isolated from a soil sample in a creek bed in a neighborhood in Indianapolis, Indiana. The soil from which the phage was isolated was scraped from the surface of the ground and was very saturated with water. The ambient temperature was approximately 29°C. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | B |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Cally is a temperate phage and is capable of producing stable lysogens. |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The phage was isolated and purified at 32°C. The plaques are turbid and have a bullseye morphology. At 24 hours, the bullseye is hard to distinguish due to the small size (≈0.15 cm). At 48 hours and beyond the bullseye is easier to see and the plaque size is larger (≈0.25 cm). When incubated at 42°C, plaques grew with a normal morphology, however there were slightly fewer plaques on the 42°C plate than on the 32°C control. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 6.2E09 pfu/mL |
| Date of SEA Lysate Titering | Oct 10, 2014 |
| Pitt Freezer Box# | 7 |
| Pitt Freezer Box Grid# | E2 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
