Mycobacterium phage CasaVerde
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Detailed Information for Phage CasaVerde
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJenna Stoneroad
Year Found2019
Location FoundElizabethville, PA United States
Finding InstitutionSaint Joseph's University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates40.5527 N, 76.8034 W Map
Discovery NotesSoil was collected on September 1st, 2019 at 8:10pm in Elizabethville, PA in the front yard of 129 Green Acres Avenue approximately 15 feet from a driveway and 20 feet from the front of the house overlooking a trailer. The soil condition was dry, dark in color and fine. Soil depth was recorded as 6.35 cm. Ambient temperature at the time of collection was 21°C or 70°F with humidity of 76% and weather conditions of cloudy, high humidity, and 3 mph SE wind.
Naming NotesThe name for this phage came from the fact that it was isolated at my house, Casa, and I wanted to incorporate my mother into the name of my phage. All throughout high school and college, students and teachers called her Kelly Verde in Spanish class so I chose Verde as a more meaningful way to incorporate her into my discovery. I put the two words together which created my phage name CasaVerde.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesPlaques were approximately 2 mm in size, extremely turbid, and circular plaques once doing three rounds of purification and a full plate titer assay.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.7×10^9 pfu/mL
Pitt Freezer Box# 101
Pitt Freezer Box Grid# G3
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload