Mycobacterium phage Coolbean
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Detailed Information for Phage Coolbean
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesSmolFry
Found ByFlorisel Rodriguez, Anissia Ojeda
Year Found2019
Location FoundCommerce City, CO United States
Finding InstitutionUniversity of Colorado at Boulder
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates39.8083 N, 104.9339 W Map
Discovery NotesCoolbean was discovered in an cold, wet environment in Commerce City, Colorado. The days following up to obtaining the soil in which Coolbean resided snow fell thus leading to the wet, cold environment of its discovery. The temperature in which the soil was recovered from was about 40 degrees Fahrenheit, with no winds present.
Naming NotesThe name Coolbean came about due to the environmental factors the phage was founded as well as the size of the plaques. The cool portion was made up as the environment that Coolbean was founded in was cold and wet. As for the Bean in Coolbean, the size of the plaques that were created were small and reminded us of beans as those are small as well.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesThe morphology of the plaques can be shown in the figures provided, and through this we were able to deduce that the discovered phage undergoes the lytic cycle. We were able to deduce that Coolbean undergoes the lytic cycle due to the presence of clear plaques. Unlike temperate phages which demonstrate a clear/cloudy center and turbid halos, Coolbean only indicates the presence of a clear center.
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
SEA Lysate Titer 2.5x10^11 pfu/mL
Available Files
Plaque PictureDownload
EM PictureDownload