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Detailed Information for Phage Cota
Discovery Information
Isolation HostPropionibacterium acnes ATCC 6919
Found ByRocky Ng
Year Found2013
Location FoundLos Angeles, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature37°C
GPS Coordinates34.068582 N, 118.441285 W Map
Discovery NotesTeam Breakphage Club (Stephen Wandro, Rocky Ng, Cynthia Mund and Fernando Morales) isolated and characterized phage Cota from a pore sample.
TA: Samantha Capati,
Instructor: Jordan Moberg Parker
Annotation was done by MIMG 103AL-F15 class Instructor: Giorgia Pirino,
Additional annotation by Rocky Ng and Jordan Moberg Parker.

Collection Date: October 4, 2013
Sample type: Biore pore strip
Sample description: Nose
Host range analysis: Able to infect laboratory P. acnes, putative P. acnes from Stephen, Cynthia, and Natalie
Naming NotesPronounced "Caught-Ah"; the team was very excited when they finally caught a phage, so Cota Phage.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedSep 11, 2015
Sequencing FacilityNC State Genomic Sciences Laboratory
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage11129
Genome length (bp)29549
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceTCGTACGGCTT
GC Content54.0%
Sequencing NotesThere is a small SNP in the population. Approximately 20% of the reads had a C instead of an A at position 14183.
Fasta file available?Yes: Download fasta file
Cluster Life CycleLytic
Lysogeny NotesBullseye morphology at higher titers.
Other Cluster Members
Annotating InstitutionUniversity of California, Los Angeles
Annotation StatusIn GenBank
Plaque NotesHigher titers have more turbid plaques, lower titers have clear plaques.
Clear; 6.3mm average.
Number of Genes48
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMN329678
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 23
Pitt Freezer Box Grid# B12
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload