Mycobacterium phage Elucidator
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| Detailed Information for Phage Elucidator | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Zachary Ricca |
| Year Found | 2014 |
| Location Found | Providence, RI USA |
| Finding Institution | Brown University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 41.826389 N, 71.381944 W Map |
| Discovery Notes | I found my phage near Providence River, in a patch of particularly dark and moist soil. I found this to be a suitable area for bacterial growth and, thus, phage propagation. In this soil, tall grasses were growing. |
| Naming Notes | I chose the name of Elucidator for my phage because the name is essentially "clearer." In this sense, it refers to the phage's ability create zones of clearing, or plaques on agar plates. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The plaques vary in size somewhat, but because of this variation persisting through 5 purifications, it is clear that I have a single phage. EM pictures only confirmed this. The morphology varies between the many 1mm plaques to large plaques as large as 3.5mm. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 2,100,000,000 pfu/ml |
| Date of SEA Lysate Titering | Oct 24, 2014 |
| Pitt Freezer Box# | 11 |
| Pitt Freezer Box Grid# | E12 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
