Mycobacterium phage EscapeArtist
EscapeArtist Plaque Picture
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Detailed Information for Phage EscapeArtist
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found BySarah C. Turner
Year Found2019
Location FoundBlacksburg, VA USA
Finding InstitutionVirginia Tech
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates37.220423 N, 80.424308 W Map
Discovery NotesI found my phage in Hahn Garden, Blacksburg VA in the middle of the circular bed. I dug down about 4 cm to get the soil sample. The soil was damp, not saturated. It was reddish-brown in appearance and was intermingled with roots of the grass.
Naming NotesI lost my phage several times when doing dilution plates. I also had a hard time getting the gel electrophoresis of my DNA to actually show bands. Even though I saw my phage under electron microscope, it was as if it was never there.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesMy plaques appeared very temperate. All the plaques were small and hard to see. There was some polymorphism in plaque sizes on some of my plates.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 102
Pitt Freezer Box Grid# A1
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload