Mycobacterium phage FieldMouse
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Detailed Information for Phage FieldMouse
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByLauren Shebby
Year Found2021
Location FoundHamburg, PA United States
Finding InstitutionLehigh University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature6°C
GPS Coordinates40.567131 N, 75.95696 W Map
Discovery NotesOn the day of soil collection, the high temperature was 44 degrees Fahrenheit and the low was 30 degrees Fahrenheit. Soil was relatively dry, but not dusty in texture. After being in a collection bag for a few days, a sapling sprouted. The sample was collected in soil adjacent to a field used for farming.
Naming NotesThe phage was named "Field Mouse" because the location of the soil sample was chosen due to a field often being the location of decaying organisms, meaning there was likely an abundance of bacteria and therefore the presence of phage. The most prominent animal at this location is a field mouse, hence the name.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesThere was variation in the head to tail ratio, so it is possible that some tails were broken, but the ratios measured were about 1:3 and 1.1:1. The tail is long and flexible and the capsid is an icosahedron shape.

The plaques were turbid and symmetrical consistently throughout plaque assays. They varied in size, but were most consistently between less than a millimeter and 3 millimeters.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload