Mycobacterium phage Flick
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| Detailed Information for Phage Flick | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Amanda Tjitro |
| Year Found | 2014 |
| Location Found | La Jolla, CA USA |
| Finding Institution | University of California, San Diego |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 32.887778 N, 117.245556 W Map |
| Discovery Notes | After enriching a sample of collected soil, plaque purification was performed six times by streaking onto LB plates. Following purification, empirical testing was conducted to find the concentration of this phage needed to attain confluent lysis. Before DNA digest, harvesting and titer calculations were performed to obtain a sufficient sample of phage. |
| Naming Notes | This phage is named after the hummingbird in the 1995 Disney film, Pocahontas. The phage has a long tail and small head, just as Flick has a long figure with a small head. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Plaque morphology changed with the incubation period of the plates. During times of longer incubation (about 5 days) plaques appeared bulls-eyed with clear centers and turbid halos. These plaques were generally 4 mm in diameter. More often than not, when shorter incubation periods were used (i.e. 2-3 days) plaques were clear and had diameters of 1.5-2 mm. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |