The Actinobacteriophage Database

Mycobacterium phage Gaia

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Detailed Information for Phage Gaia
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Benjamin Yoder
Year Found	2013
Location Found	Pittsburgh, PA USA
Finding Institution	University of Pittsburgh
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	40.446389 N, 79.9575 W Map
Naming Notes	From the Greek being that represented mother earth.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Nov 13, 2013
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina Sequencing
Approximate Shotgun Coverage	3247
Genome length (bp)	90460
Character of genome ends	3' Sticky Overhang
Overhang Length	10 bases
Overhang Sequence	CGGCCAGCTG
GC Content	56.8%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	X
Subcluster	--
Cluster Life Cycle	Temperate
Lysogeny Notes	This phage has only made leaky lysogens so far.
Other Cluster Members	Click to View Gaia Nebkiss
Annotating Institution	Unknown or unassigned
Annotation Status	In GenBank
Plaque Notes	The mycobacteriophage Gaia forms small clear plaques at 30 degrees Celsius. However at 37 degrees Celsius the plaques become a little smaller and less numerous. At 37 the plaques also become cloudy instead of perfectly clear.
Morphotype	Siphoviridae
Has been Phamerated?	Yes
Gene List	Click to View Gaia_1 Gaia_2 (terminase large subunit) Gaia_3 (portal protein) Gaia_4 Gaia_5 (major capsid protein) Gaia_6 (head-to-tail adaptor) Gaia_7 Gaia_8 Gaia_9 (minor tail protein) Gaia_10 (tail assembly chaperone) Gaia_11 (tail assembly chaperone) Gaia_12 (tape measure protein) Gaia_13 (minor tail protein) Gaia_14 (minor tail protein) Gaia_15 Gaia_16 (minor tail protein) Gaia_17 (homoendonuclease) Gaia_18 (homoendonuclease) Gaia_19 Gaia_20 (minor tail protein) Gaia_21 (minor tail protein) Gaia_22 (minor tail protein) Gaia_23 (minor tail protein) Gaia_24 (minor tail protein) Gaia_25 Gaia_26 (minor tail protein) Gaia_27 (minor tail protein) Gaia_28 (HTH domain protein) Gaia_29 Gaia_30 (DNA binding domain protein) Gaia_31 Gaia_32 (lysin A) Gaia_33 Gaia_34 (lysin B) Gaia_35 Gaia_36 Gaia_37 Gaia_38 Gaia_39 Gaia_40 Gaia_41 Gaia_42 Gaia_43 (Lsr2-like protein) Gaia_44 Gaia_45 Gaia_46 Gaia_47 Gaia_48 Gaia_49 Gaia_50 Gaia_51 (excise) Gaia_52 (integrase) Gaia_54 Gaia_55 Gaia_56 Gaia_57 Gaia_58 Gaia_59 Gaia_60 Gaia_61 Gaia_62 Gaia_63 Gaia_64 Gaia_65 Gaia_66 (HTH domain protein) Gaia_67 Gaia_68 (transcriptional regulator) Gaia_69 (HTH binding protein) Gaia_70 (hydrolase) Gaia_71 Gaia_72 (WhiB) Gaia_73 Gaia_74 Gaia_75 Gaia_76 Gaia_77 Gaia_78 Gaia_79 Gaia_80 Gaia_81 Gaia_82 Gaia_83 Gaia_84 Gaia_85 Gaia_86 Gaia_87 Gaia_88 (recombination endonuclease VII) Gaia_89 (ribonucleotide reductase) Gaia_90 (ThyX-like thymidylate synthase) Gaia_91 Gaia_92 Gaia_93 Gaia_94 (polymerase primase) Gaia_95 Gaia_96 Gaia_97 Gaia_98 Gaia_99 Gaia_100 Gaia_101 Gaia_102 (helicase) Gaia_103 (PAPS reductase) Gaia_104 (ectoine synthase/ParB multidomain protein) Gaia_105 Gaia_106 Gaia_107 Gaia_108 Gaia_109 (RepA-like helicase) Gaia_110 Gaia_111 Gaia_112 Gaia_113 Gaia_114 Gaia_115 Gaia_116 Gaia_117 Gaia_118 (ClpP-like protease) Gaia_119 (DnaQ-like exonuclease) Gaia_120 Gaia_121 Gaia_122 Gaia_123 Gaia_124 Gaia_125 Gaia_126 Gaia_127 Gaia_128 Gaia_129 (DnaQ-like DNA polymerase III subunit) Gaia_130 Gaia_131 Gaia_132 Gaia_133 Gaia_134 Gaia_135 Gaia_136 Gaia_137 Gaia_138 Gaia_139 Gaia_140 (restriction endonuclease, HNH endonuclease domain) Gaia_141 Gaia_142 Gaia_143 (metallophosphoesterase) Gaia_144 Gaia_145 Gaia_147 Gaia_150 Gaia_151 Gaia_152 Gaia_153 Gaia_154 Gaia_155 Gaia_156 Gaia_157 Gaia_158 Gaia_159 Gaia_160 Gaia_161 Gaia_162 Gaia_163 Gaia_164 Gaia_165 Gaia_166 Gaia_167 Gaia_168 Gaia_169 (homing endonuclease) Gaia_170 Gaia_171 (methyltransferase) Gaia_172 (hydrolase) Gaia_173 Gaia_174 Gaia_175 Gaia_176 Gaia_177 Gaia_178 Gaia_179 Gaia_180 Gaia_181 Gaia_182 Gaia_183
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	KJ567043
Refseq Number	NC_026590
Published in Paper	Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	1
Pitt Freezer Box Grid#	A12
Available Files
Plaque Picture	Download
EM Picture	Download
Final DNAMaster File	Download

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