Microbacterium phage GardenState
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Detailed Information for Phage GardenState
Discovery Information
Isolation HostMicrobacterium sp. ISAT203
Found ByPaulina Onisko
Year Found2019
Location FoundCranford, NJ United States
Finding InstitutionJames Madison University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates40.6584 N, 74.2996 W Map
Discovery NotesThis phage was discovered in the soil from New Jersey. It was discovered through being enriched with Microbacterium. After being enriched with Microbacterium and becoming evident on the Luria Broth Agar plate as a bacteriophage, the phage was picked, replicated, diluted through a dilution series, picked through passaging, and a full plate infection was done on it.
Naming NotesThe name of this phage came from the place and state it was discovered in. This phage was discovered in my backyard garden in New Jersey, which is often referred to as the "Garden State."
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 14, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1087
Genome length (bp)45424
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCGGGGAGGCAT
GC Content70.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEH
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionJames Madison University
Annotation StatusIn GenBank
Plaque NotesThis plaque usually has large circular spots which are clear. The spots often have a large cloudy circular ring surrounding each one.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

GardenState_1
(terminase small subunit)

GardenState_2
(terminase large subunit)

GardenState_3
(portal protein)

GardenState_4
(capsid maturation protease and MuF-like fusion protein)

GardenState_5

GardenState_6
(scaffolding protein)

GardenState_7
(major capsid protein)

GardenState_8
(head-to-tail adaptor)

GardenState_9

GardenState_10

GardenState_11
(tail terminator)

GardenState_12
(major tail protein)

GardenState_13
(tail assembly chaperone)

GardenState_14
(tail assembly chaperone)

GardenState_15
(tape measure protein)

GardenState_16
(minor tail protein)

GardenState_17
(minor tail protein)

GardenState_18

GardenState_19
(minor tail protein)

GardenState_20
(minor tail protein)

GardenState_21
(endolysin)

GardenState_22

GardenState_23
(holin)

GardenState_24

GardenState_25

GardenState_26

GardenState_27
(deoxynucleoside monophosphate kinase)

GardenState_28

GardenState_29
(exonuclease)

GardenState_30
(nucleoside deoxyribosyltransferase)

GardenState_31

GardenState_32
(LAGLIDADG endonuclease)

GardenState_33

GardenState_34

GardenState_35

GardenState_36

GardenState_37

GardenState_38

GardenState_39

GardenState_40
(recombination directionality factor)

GardenState_41

GardenState_42
(NrdH-like glutaredoxin)

GardenState_43

GardenState_44
(holliday junction resolvase)

GardenState_45
(DNA primase/helicase)

GardenState_46

GardenState_47

GardenState_48
(DNA polymerase I)

GardenState_49

GardenState_50
(RNA polymerase sigma factor)

GardenState_51

GardenState_52

GardenState_53
(SprT-like protease)

GardenState_54

GardenState_55

GardenState_56

GardenState_57

GardenState_58
(serine integrase)

GardenState_59

GardenState_60

GardenState_61

GardenState_62

GardenState_63

GardenState_64

GardenState_65

GardenState_66

GardenState_67

GardenState_68

GardenState_69
(ssDNA-binding protein)

GardenState_70

GardenState_71

GardenState_72

GardenState_73

GardenState_74

GardenState_75

GardenState_76
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT952845
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload
GenBank File for PhameratorDownload