Microbacterium phage GardenState
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Detailed Information for Phage GardenState
Discovery Information
Isolation HostMicrobacterium sp. ISAT203
Found ByPaulina Onisko
Year Found2019
Location FoundCranford, NJ United States
Finding InstitutionJames Madison University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates40.6584 N, 74.2996 W Map
Discovery NotesThis phage was discovered in the soil from New Jersey. It was discovered through being enriched with Microbacterium. After being enriched with Microbacterium and becoming evident on the Luria Broth Agar plate as a bacteriophage, the phage was picked, replicated, diluted through a dilution series, picked through passaging, and a full plate infection was done on it.
Naming NotesThe name of this phage came from the place and state it was discovered in. This phage was discovered in my backyard garden in New Jersey, which is often referred to as the "Garden State."
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 14, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1087
Genome length (bp)45424
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCGGGGAGGCAT
GC Content70.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEH
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionJames Madison University
Annotation StatusAwaiting_QC
Plaque NotesThis plaque usually has large circular spots which are clear. The spots often have a large cloudy circular ring surrounding each one.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

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Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload