| Detailed Information for Phage Geostin | |
| Discovery Information | |
| Isolation Host | Streptomyces scabiei RL-34 |
| Found By | Ihunanya Nwoji & Jessica Schultz |
| Year Found | 2018 |
| Location Found | Pasadena, MD USA |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 28°C |
| GPS Coordinates | 39.140777 N, 76.528469 W Map |
| Discovery Notes | Isolated from soil collected 9/11/18 from the surface of a grassy hill. Soil was damp and sandy and collected on an overcast day with 93% humidity and an ambient temperature of 23 °C. |
| Naming Notes | The phage name is derived from the name of the compound geosmin, which is produced by streptomycetes and is responsible for the distinct scent of soil. The ending of the name comes from the beginning letters of Tick Neck Park, where the phage was found. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 24, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1661 |
| Genome length (bp) | 46175 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 253 bp |
| GC Content | 60.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | BF |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members | |
| Annotating Institution | University of Maryland, Baltimore County |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are about 1.1 mm in diameter and have clear centers and turbid edges when grown at 28 °C for 24 hours on S. scabiei on NA+. |
| Morphotype | Podoviridae |
| Number of Genes | 66 |
| Number of tRNAs | 16 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewGeostin_1 Geostin_2 Geostin_3 Geostin_4 Geostin_5 Geostin_6 Geostin_7 Geostin_8 Geostin_9 Geostin_10 Geostin_11 Geostin_12 Geostin_13 Geostin_30 Geostin_31 Geostin_32 Geostin_33 Geostin_34 Geostin_35 Geostin_36 Geostin_37 Geostin_38 Geostin_39 Geostin_40 Geostin_41 Geostin_42 Geostin_43 Geostin_44 Geostin_45 Geostin_46 Geostin_47 Geostin_48 Geostin_49 Geostin_50 Geostin_51 Geostin_52 Geostin_53 Geostin_54 Geostin_55 Geostin_56 Geostin_57 Geostin_58 Geostin_59 Geostin_60 Geostin_61 Geostin_62 Geostin_63 Geostin_64 Geostin_65 Geostin_66 Geostin_67 Geostin_68 Geostin_69 Geostin_70 Geostin_71 Geostin_72 Geostin_73 Geostin_74 Geostin_75 Geostin_76 Geostin_77 Geostin_78 Geostin_79 Geostin_80 Geostin_81 Geostin_82 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN119376 |
| Refseq Number | None yet |
| Published in Paper | Davis et al 2022, Microbiology Resource Announcements: Genome Sequences of Streptomyces Bacteriophages Fabian, FlowerPower, Geostin, RetrieverFever, and Vorvolakos |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 4x10^8 pfu/mL |
| Pitt Freezer Box# | 83 |
| Pitt Freezer Box Grid# | F4 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
