Mycobacterium phage Hard2get
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| Detailed Information for Phage Hard2get | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | Jonseena |
| Found By | David Wen |
| Year Found | 2015 |
| Location Found | Baltimore, MD USA |
| Finding Institution | Johns Hopkins University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 39.3289 N, 76.6203 W Map |
| Discovery Notes | This Phage was discovered in the Community Garden at Johns Hopkins University. It was obtained near a sunflower and a tomato plant with temperatures at approximately 30 degrees celsius. The day was clear and the soil that the phage was obtained form was relatively dry. The soil was taken from the surface. |
| Naming Notes | One will inevitably face some type of difficulty when performing the scientific research method; it's unavoidable even to master researchers. To overcome these difficulties, it is imperative that one recognizes errors and finds ways to make corrections. In other words, keep moving forward. For me, purifying and titering this phage took far more creativity than the lab manual suggested; truly, it seemed that I was stopped during each step of the purifying and titering process because something needed to be fixed or the results were inadequate. From using formaldehyde to repeating platings to minimizing the amount of phage buffer used, every step required something a little extra. In all honesty, although conducting the experiment was difficult, naming was arguably the most difficult part. Thus, I decided to name it based on exactly what it is: Hard2get |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | C |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The plaques that appear on the plating were homogenously about 1mm in diameter, very small plaques with very small halos. On a plate, they appear to simply be small dots. |
| Morphotype | Myoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.6 * 10^-6 |
| Date of SEA Lysate Titering | Oct 1, 2015 |
| Pitt Freezer Box# | 71 |
| Pitt Freezer Box Grid# | D1 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |