Mycobacterium phage HarryStyless
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| Detailed Information for Phage HarryStyless | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Arizona Marakovits |
| Year Found | 2023 |
| Location Found | Marlton, NJ United States |
| Finding Institution | Saint Joseph's University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 39.888785 N, 74.917712 W Map |
| Discovery Notes | Found at the bottom of my compost, had egg shells and other organic material The soil contents that the phage was found in came from Owen McAvaddy The sample was collected on February 5, 2023, at approximately 1:00 pm The temperature was around 10 C (50 F) The soil was dark and moist enough to shape/mold, but also broke apart easily |
| Naming Notes | Named after my favorite artist that I listened to most of the time while experimenting on this phage |
| Sequencing Information | |
| Sequencing Complete? | No |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | From the first round of purification, I found that the plaques that arose from purification were clear and required 48 hr of incubation. There were a total of 3 rounds of purification that produced reliable results. While continuing with the experiment, the MTL prep produced plaques that measured in diameter from 2 mm-3 mm. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 166 |
| Pitt Freezer Box Grid# | E11 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |