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Mycobacterium phage Helpful
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Detailed Information for Phage Helpful
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByEsther Jakubowicz, Keverene Mason
Year Found2017
Location FoundSuffern, NY USA
Finding InstitutionRockland Community College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates41.13363 N, 74.086268 W Map
Discovery NotesSept 18, 2017, 12 pm. Sunny day, temps at 24 degrees Celsius. Collected from Peace Garden, on RCC campus at approx. 6 cm deep.
Naming NotesWe named it after our Project's group name, Helpful Pests.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 5, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage947
Genome length (bp)64794
Character of genome endsCircularly Permuted
GC Content59.6%
Sequencing Notessample contains a mixed population, 2% of reads are Cluster B. Sample will need to be purified and re-archived before annotation

at position 6809 bp approximately 20% of reads contain a T instead of consensus C.
Fasta file available?Yes: Download fasta file
Characterization
ClusterD
SubclusterD1
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionUniversity of California, Los Angeles
Annotation StatusIn GenBank
Plaque NotesTwo morphologies noted. One larger, oval shaped 2 mm. The second one is a smaller, round, pinpoint plaque 1 mm.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

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(VIP2-like toxin)

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(terminase)

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(portal protein)

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(metalloprotease)

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(MuF-like minor capsid protein)

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(major capsid protein)

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(tail terminator)

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(major tail protein)

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(tail assembly chaperone)

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(tail assembly chaperone)

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(tape measure protein)

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(minor tail protein)

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(minor tail protein)

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(minor tail protein)

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(minor tail protein)

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(minor tail protein)

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(lysin A)

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(lysin B)

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(DNA helicase)

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(Cas4 family exonuclease)

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(RecA-like DNA recombinase)

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(hydrolase)

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(methyltransferase)

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(FabG-like reductase)

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(DnaE-like DNA polymerase III)

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(ThyX-like thymidylate synthase)

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(RuvC-like resolvase)

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(helix-turn-helix DNA binding domain protein)

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(DNA primase/polymerase)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMN284908
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 66
Pitt Freezer Box Grid# H11
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload