Mycobacterium phage HotSauce
Know something about this phage that we don't? Modify its data.
| Detailed Information for Phage HotSauce | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Mitch Thielemann and Hallie Kurtz |
| Year Found | 2013 |
| Location Found | Spokane, WA USA |
| Finding Institution | Gonzaga University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 47.666948 N, 117.402773 W Map |
| Discovery Notes | The soil was collected from a flower bed on a cloudy day at 10:15 AM on September 21, 2013. The when collected the soil was slightly moist. |
| Naming Notes | We chose the name HotSauce because we had a frustrating time isolating a phage. Throughout the lab we joked that the phage was "too cool" to grow. We developed the nickname "HotSauce" because of this, and the nickname stuck. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Plaques created by HotSauce are small and circular approximately 0.2mm in diameter. After our second round of streaking to isolate the our plaques took on a slight morphology change. In order to ensure that we had isolated ONE phage, we streaked our plaques again. From this we cultivated plates with plaques uniform in shape and size. The capsid length of the phage is 72.2 nm, and the tail length is 238.9 nm, making the total length 311.2 nm. Furthermore, our plaques were grown at room temperature (~25 degrees Celsius). When we ran a trial at 37 degrees Celsius, no plaques were seen, indicating that our phage is temperature specific. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 6 |
| Pitt Freezer Box Grid# | B10 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |