Mycobacterium phage Huphlepuff
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Detailed Information for Phage Huphlepuff
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByDalton Schengrund and Anna Anand
Year Found2021
Location FoundPhiladelphia, PA United States
Finding InstitutionUniversity of the Sciences in Philadelphia
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates39.946663 N, 75.208668 W Map
Discovery NotesDepth - 1 cm
Moisture - Moist
Located in a flower bed next to the security entrance at Griffith Hall at USciences. Very close to roads (~5 feet away), in a suburban area. Ground is not sloped and no foot traffic should be taking place in the area of collection.
Naming NotesNamed after Anna's Hogwarts house, Hufflepuff, but with a bit of zest from the ph- in "phage".
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 26, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage32
Genome length (bp)64543
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceGCGCGCAGCGC
GC Content63.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterS
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionUniversity of the Sciences in Philadelphia
Annotation StatusQC Complete
Plaque NotesGenerates three different types of morphologies; small, turbid plaques; large, turbid plaques; and relatively larger plaques that form a bullseye/halo morphology.

The titer collected on 12/2/21 read to be 1.12 x 10^7 pfu
Has been Phamerated?Yes
Gene List

Click to View

Huphlepuff_1

Huphlepuff_2

Huphlepuff_3

Huphlepuff_4

Huphlepuff_5

Huphlepuff_6

Huphlepuff_7

Huphlepuff_8

Huphlepuff_9

Huphlepuff_10

Huphlepuff_11

Huphlepuff_12
(MazG-like nucleotide pyrophosphohydrolase)

Huphlepuff_13

Huphlepuff_14

Huphlepuff_15

Huphlepuff_16

Huphlepuff_17

Huphlepuff_18

Huphlepuff_19

Huphlepuff_20

Huphlepuff_21

Huphlepuff_22

Huphlepuff_23

Huphlepuff_24

Huphlepuff_25
(helix-turn-helix DNA binding domain)

Huphlepuff_26

Huphlepuff_27

Huphlepuff_28

Huphlepuff_29

Huphlepuff_30

Huphlepuff_31

Huphlepuff_32

Huphlepuff_33

Huphlepuff_34
(DNA methyltransferase)

Huphlepuff_35
(terminase, small subunit)

Huphlepuff_36

Huphlepuff_37
(terminase, large subunit)

Huphlepuff_38
(portal protein)

Huphlepuff_39

Huphlepuff_40
(scaffolding protein)

Huphlepuff_41
(capsid decoration protein)

Huphlepuff_42
(major capsid protein)

Huphlepuff_43
(head-to-tail adaptor)

Huphlepuff_44
(head-to-tail stopper)

Huphlepuff_45

Huphlepuff_46
(tail terminator)

Huphlepuff_47
(major tail protein)

Huphlepuff_48
(tail assembly chaperone)

Huphlepuff_49
(tail assembly chaperone)

Huphlepuff_50
(tape measure protein)

Huphlepuff_51
(minor tail protein)

Huphlepuff_52
(minor tail protein)

Huphlepuff_53
(HNH endonuclease)

Huphlepuff_54

Huphlepuff_55

Huphlepuff_56
(lysin A)

Huphlepuff_57

Huphlepuff_58
(lysin B)

Huphlepuff_59
(holin)

Huphlepuff_60

Huphlepuff_61

Huphlepuff_62

Huphlepuff_63
(hydrolase)

Huphlepuff_64
(helix-turn-helix DNA binding domain)

Huphlepuff_65

Huphlepuff_66

Huphlepuff_67
(helix-turn-helix DNA binding domain)

Huphlepuff_68
(Cas4 family exonuclease)

Huphlepuff_69

Huphlepuff_70

Huphlepuff_71
(HNH endonuclease)

Huphlepuff_72

Huphlepuff_73

Huphlepuff_74

Huphlepuff_75

Huphlepuff_76

Huphlepuff_77
(RepA-like replication initiator)

Huphlepuff_78

Huphlepuff_79

Huphlepuff_80

Huphlepuff_81

Huphlepuff_82

Huphlepuff_83

Huphlepuff_84

Huphlepuff_85

Huphlepuff_86

Huphlepuff_87
(O-methyltransferase)

Huphlepuff_88
(glycosyltransferase)

Huphlepuff_89

Huphlepuff_90

Huphlepuff_91
(glycosyltransferase)

Huphlepuff_92

Huphlepuff_93

Huphlepuff_94
(HNH endonuclease)

Huphlepuff_95
(minor tail protein)

Huphlepuff_96
(minor tail protein)

Huphlepuff_97

Huphlepuff_98

Huphlepuff_99

Huphlepuff_100

Huphlepuff_101

Huphlepuff_102

Huphlepuff_103

Huphlepuff_104

Huphlepuff_105

Huphlepuff_106

Huphlepuff_107

Huphlepuff_108

Huphlepuff_109

Huphlepuff_110

Huphlepuff_111

Huphlepuff_112
(glutamine amidotransferase domain)

Huphlepuff_113
(glutamine amidotransferase domain)

Huphlepuff_114

Huphlepuff_115

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 136
Pitt Freezer Box Grid# A10
Available Files
Plaque PictureDownload
GenBank File for PhameratorDownload