Mycobacterium phage Inligno
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Detailed Information for Phage Inligno
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesTheBaum
Found ByVeda and Thao
Year Found2019
Location FoundRichardson, TX USA
Finding InstitutionCollin College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates32.9483 N, 96.7229 W Map
Discovery NotesSoil was collected at 11:00 A.M from under the mulch surrounding a tree in a backyard. The weather was cloudy with 90% humidity and the soil was fairly wet and moist after a quick morning shower. The temperature during the soil collection was 40 degrees Fahrenheit. Although the soil was moist and wet it did contain some large soil particles such as woodchips and some pebbles.
Naming NotesWe obtained our sample from underneath a tree. We decided to name our phage "Tree" in Latin which would be "Inligno" because we wanted to name our phage from the location it was isolated from.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesOur phage is lysogenic because the plaques are not very clear and are kind of blurry. The plaque shape is a little bit shaped like an oval and is sized 0.2 cm. in diameter. The plaques would be described as turbid and not a representation of a lytic phase. Except when the amplification protocol was followed through our phage entered the lytic phase due to the increase in the number of phages.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1x10^10 pfu/ml
Pitt Freezer Box# 105
Pitt Freezer Box Grid# C6
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload