Mycobacterium phage KanyeSouth
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| Detailed Information for Phage KanyeSouth | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Joseph "Brad" Segal |
| Year Found | 2008 |
| Location Found | San Diego, CA USA |
| Finding Institution | University of California, San Diego |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 32.8675 N, 117.24298 W Map |
| Discovery Notes | Taken from soil one inch below surface approx 25°C, in an area of mostly shaded grass at the south end of Muir Field. Healthy grass is surrounding it, but large patch of dirt has no big stands of grass growing in it, just multiple tiny wasps of baby grass. Sample from slanted surface that faces South. |
| Growth Notes | Titer was increased due to UV exposure. 10 plates were exposed to short-wave UV radiation for two minutes each and then 8mL of phage buffer was immediately added to each plate. Plates were stored at 4°C for 22 hours before removing and filtering lysate. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Exact plaque morphology varies each time that plaque is plated, but for the most part the plaque will appear extremely turbid. The plaques will range from 1mm-2mm diameter. Plaques incubated from 24-48 hours at 37°C. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Designator | KanyeSouth |
| SEA Lysate Titer | 4.00e09 |
| Pitt Freezer Box# | 6 |
| Pitt Freezer Box Grid# | 10 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |