Mycobacterium phage Katy131
Katy131 Plaque Picture
Katy131 EM Picture
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Detailed Information for Phage Katy131
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByTiara Cochran
Year Found2015
Location FoundLiberty, KY USA
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates37.412 N, 84.989 W Map
Discovery NotesThe purification process was very tedious because I believed each sample was impure. It wasn't until multiple rounds of purifications were performed that we explored the possibility of clear plaque mutants. This was verified by streaking each plaque onto separate plates.
Naming NotesThe phage was named after Barbara Streisand's character in the movie "The Way We Were." She was a very complicated character, as was this phage.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesPlaques present as turbid but produce clear mutants the longer the plates are incubated. The turbid plaques are 1mm in diameter and the clear plaque mutants are 1.5mm in diameter.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Designator 2015WEKUKaty131TLC
SEA Lysate Titer 5.00x10^9
Date of SEA Lysate Titering Nov 3, 2015
Pitt Freezer Box# 27
Pitt Freezer Box Grid# F2
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload