| Detailed Information for Phage Leopard | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Deborah Duong |
| Year Found | 2021 |
| Location Found | Philadelphia, PA United States of America |
| Finding Institution | Saint Joseph's University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 40.066 N, 75.048 W Map |
| Discovery Notes | The sample was collected on January 23, 2021, at around 11:30 AM in a flower bed at an apartment complex near Pennypack Woods. The weather that day was sunny but windy. The sample was collected after digging about 2cm deep into the soil. After conducting a PCR test for the D cluster, results suggest it is unlikely that Leopard is in the D cluster. |
| Naming Notes | Leopard is the name of the phage because when purifying, the phage looked similar to a leopard print, and was a description I used in my lab notebook frequently. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 18, 2021 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2095 |
| Genome length (bp) | 71575 |
| Character of genome ends | Circularly Permuted |
| GC Content | 58.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AE |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members | |
| Annotating Institution | Saint Joseph's University |
| Annotation Status | In GenBank |
| Plaque Notes | Leopard took about 48 hours of incubation before plaques appear on plates. The plaques are turbid, and range from 0.5 - 1.5 mm in diameter. From measuring 8 different phages on EM pictures, the average capsid size was 54.4nm and the average tail length was 232.5nm. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewLeopard_1 Leopard_2 Leopard_3 Leopard_4 Leopard_5 Leopard_6 Leopard_7 Leopard_8 Leopard_9 Leopard_10 Leopard_11 Leopard_12 Leopard_13 Leopard_14 Leopard_15 Leopard_16 Leopard_17 Leopard_18 Leopard_19 Leopard_20 Leopard_21 Leopard_22 Leopard_23 Leopard_24 Leopard_25 Leopard_26 Leopard_27 Leopard_28 Leopard_29 Leopard_30 Leopard_31 Leopard_32 Leopard_33 Leopard_34 Leopard_35 Leopard_36 Leopard_37 Leopard_38 Leopard_39 Leopard_40 Leopard_41 Leopard_42 Leopard_43 Leopard_44 Leopard_45 Leopard_46 Leopard_47 Leopard_48 Leopard_49 Leopard_50 Leopard_51 Leopard_52 Leopard_53 Leopard_54 Leopard_55 Leopard_56 Leopard_57 Leopard_58 Leopard_59 Leopard_60 Leopard_61 Leopard_62 Leopard_63 Leopard_64 Leopard_65 Leopard_66 Leopard_67 Leopard_68 Leopard_69 Leopard_70 Leopard_71 Leopard_72 Leopard_73 Leopard_74 Leopard_75 Leopard_76 Leopard_77 Leopard_78 Leopard_79 Leopard_80 Leopard_81 Leopard_82 Leopard_83 Leopard_84 Leopard_85 Leopard_86 Leopard_87 Leopard_88 Leopard_89 Leopard_90 Leopard_91 Leopard_92 Leopard_93 Leopard_94 Leopard_95 Leopard_96 Leopard_97 Leopard_98 Leopard_99 Leopard_100 Leopard_101 Leopard_102 Leopard_103 Leopard_104 Leopard_105 Leopard_106 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON108643 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 129 |
| Pitt Freezer Box Grid# | H9 |
| Available Files | |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
