Mycobacterium phage LittleLaf
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Detailed Information for Phage LittleLaf
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByRachael Broder
Year Found2016
Location FoundEaston, PA USA
Finding InstitutionLafayette College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates40.697293 N, 75.210184 W Map
Discovery NotesDiscovered on the Lafayette College campus near the Kirby Hall of Civil Rights close to the building, on the side of a hill, close to a flower bed.
Naming NotesMy phage is very small and I go to Lafayette College so it is called LittleLaf.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 9, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1286
Genome length (bp)64834
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceGCGCGCAGCGC
GC Content63.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterS
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesThe plaques are very small and almost perfectly circular.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

LittleLaf_1

LittleLaf_2

LittleLaf_3

LittleLaf_4

LittleLaf_5

LittleLaf_6

LittleLaf_7

LittleLaf_8

LittleLaf_9

LittleLaf_10

LittleLaf_11

LittleLaf_12

LittleLaf_13

LittleLaf_14

LittleLaf_15

LittleLaf_16

LittleLaf_17

LittleLaf_18

LittleLaf_19

LittleLaf_20

LittleLaf_21

LittleLaf_22

LittleLaf_23

LittleLaf_24

LittleLaf_25

LittleLaf_26

LittleLaf_27

LittleLaf_28

LittleLaf_29

LittleLaf_30

LittleLaf_31
(HNH endonuclease)

LittleLaf_32
(DNA methylase)

LittleLaf_33

LittleLaf_34

LittleLaf_35
(terminase)

LittleLaf_36
(portal protein)

LittleLaf_37
(MuF-like minor capsid protein)

LittleLaf_38
(capsid maturation protease)

LittleLaf_39
(minor capsid protein)

LittleLaf_40
(major capsid protein)

LittleLaf_41
(head-to-tail connector complex protein)

LittleLaf_42

LittleLaf_43

LittleLaf_44
(head-to-tail connector complex protein)

LittleLaf_45
(major tail subunit)

LittleLaf_46
(tail assembly chaperone)

LittleLaf_47
(tail assembly chaperone)

LittleLaf_48
(tape measure protein)

LittleLaf_49
(minor tail protein)

LittleLaf_50
(minor tail protein)

LittleLaf_51
(HNH endonuclease)

LittleLaf_52

LittleLaf_53

LittleLaf_54
(lysin A)

LittleLaf_55

LittleLaf_56
(lysin B)

LittleLaf_57
(holin)

LittleLaf_58

LittleLaf_59

LittleLaf_60

LittleLaf_61

LittleLaf_62

LittleLaf_63

LittleLaf_64

LittleLaf_65
(HTH DNA binding protein)

LittleLaf_66
(exonuclease)

LittleLaf_67

LittleLaf_68

LittleLaf_69
(HNH endonuclease)

LittleLaf_70

LittleLaf_71

LittleLaf_72

LittleLaf_73

LittleLaf_74

LittleLaf_75

LittleLaf_76

LittleLaf_77

LittleLaf_78

LittleLaf_79

LittleLaf_80

LittleLaf_81

LittleLaf_82

LittleLaf_83
(O-methyltransferase)

LittleLaf_84
(methyltransferase)

LittleLaf_85
(galactosyltransferase)

LittleLaf_86

LittleLaf_87
(glycosyltransferase)

LittleLaf_88

LittleLaf_89

LittleLaf_90
(HNH endonuclease)

LittleLaf_91
(minor tail protein)

LittleLaf_92
(minor tail protein)

LittleLaf_93

LittleLaf_94

LittleLaf_95

LittleLaf_96

LittleLaf_97

LittleLaf_98

LittleLaf_99

LittleLaf_100

LittleLaf_101

LittleLaf_102

LittleLaf_103

LittleLaf_104

LittleLaf_105

LittleLaf_106

LittleLaf_107

LittleLaf_108

LittleLaf_109
(glucosamine 6-phosphate synthetase)

LittleLaf_110

LittleLaf_111

LittleLaf_112

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH727553
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.67x10^8
Date of SEA Lysate Titering Nov 8, 2016
Pitt Freezer Box# 30
Pitt Freezer Box Grid# H5
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload