Gordonia phage LittleRascal
LittleRascal EM Picture
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Detailed Information for Phage LittleRascal
Discovery Information
Isolation HostGordonia rubripertincta NRRL B-16540
Found ByEmre Nalbantoglu
Year Found2021
Location FoundFairfax, VA United States
Finding InstitutionGeorge Mason University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature-2°C
GPS Coordinates38.828637 N, 77.30683 W Map
Discovery NotesThis phage was collected on 01/29/2021 at 10:55 am in the gardens of Innovation Hall at George Mason University's Fairfax Campus. The garden topsoil sample collected was moist and a bit frozen. Soil was sandy, and no organic matter was present. Sample was collected about 2 inches below the surface.
Naming NotesI named the phage LittleRascal because it was very challenging to purify. DNA isolation was also very trying.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesAt first, while the phage was in the lytic phase, there was only one plaque morphology. Plaques were tiny and clear, and they had well-defined borders. Then, after the last round of purification, the phage began showing two plaque morphologies, one lytic and the other lysogenic. The lysogenic morphology was bigger and turbid.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives