Mycobacterium phage Luboph
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| Detailed Information for Phage Luboph | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Parker Sevier |
| Year Found | 2017 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 34.0665 N, 118.452 W Map |
| Discovery Notes | Phage Luboph was isolated by Parker Sevier on 4/11/17 from moist surface soil collected from urban area, damp with plant growth. Air temp at time of collection was 20C. Luboph was isolated by enrichment plating at 30C (10X 7H9/glycerol medium, CaCl2, AD Supplement, M. smeg). Characterization was performed by Team Natural Cellection (Nikolina Walas, Aika Miikeda, Lauren Brenner, Sai Alam) and Team Sick Virions (Alice Sukhina, Jinhua Shen, Andy Wei, Crystal Ma). Phage Luboph appears to be a temperate phage that demonstrated turbid or clear plaque morphologies under different plating conditions. Turbid plaques occur consistently at 35˚C and 37˚C, and clear plaques are seen at 30˚C . Putative lysogen were isolated that release phage. Name of Individual who collected sample: Parker Sevier Midi-Lysate (Archive) Titer: 1x10e11 PFU/mL Date of Midi-Lysate Titer: 5/10/17 Instructor: Dr. Jordan Moberg Parker Teaching Assistant: Michelle Bina Undergraduate Assistant: Rachel Zhu |
| Naming Notes | Luboph is an English alphabet phonetical representation of a Russian word that means Love. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Phage Luboph appears to be a temperate phage that demonstrated turbid or clear plaque morphologies under different plating conditions. Turbid plaques occur consistently at 35˚C and 37˚C, and clear plaques are seen at 30˚C . Average size (and size range) diameter in mm: 3mm (1.0 - 6.0 mm in various environments but most consistently 3 mm) |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 45 |
| Pitt Freezer Box Grid# | D8 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
