| Detailed Information for Phage LukeCage |
| Discovery Information |
| Isolation Host | Streptomyces griseus ATCC 10137 |
| Found By | Ben Gillick |
| Year Found | 2017 |
| Location Found | Dallas, TX USA |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 28°C |
| GPS Coordinates | 32.749208 N, 96.95602 W Map |
| Discovery Notes | Soil was taken on September 4th, 2017 at approximately 10:30pm.
Despite having rained that morning, the soil was loose and dry, containing a small amount of loose debris(small pieces of leaves, small twigs)
|
| Naming Notes | Luke Cage is a cool marvel character. Also cage rhymes with phage so that's fun. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 14, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Genome length (bp) | 133195 |
| Character of genome ends | Unknown |
| Direct Terminal Repeat Length | 12291 bp |
| GC Content | 49.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BE |
| Subcluster | BE2 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
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| Annotating Institution | University of North Texas |
| Annotation Status | In GenBank |
| Plaque Notes | Nearly all plaques were circular and symmetrical, averaging between .9 and 1.1 mm in diameter. They are all very clear with very little turbidity. Some plaques around the edges are in close proximity and come together which gives the illusion of slightly turbid edges. In plaques around edges circular shape is not as uniform and plaques look slightly more free-form than a typical circle due to the higher density in this area. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH590597 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
70 |
| Pitt Freezer Box Grid# |
C5 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
