Gordonia phage Madeline
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Detailed Information for Phage Madeline
Discovery Information
Isolation HostGordonia terrae 3612
Found ByMadeline Nisperos
Year Found2018
Location FoundPITTSBURGH, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates40.444444 N, 79.948889 W Map
Discovery NotesThe sample was collected from a planter that contained a sparse collection of flowers and leafy plants. The soil was dry and crumbled easily.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 3, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage93
Genome length (bp)51668
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGCTGGGGA
GC Content66.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCZ
SubclusterCZ1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque NotesThe phage I found produced clear and small plaques around 2 mm in diameter.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Madeline_1
(terminase small subunit)

Madeline_2
(terminase large subunit)

Madeline_3
(membrane protein)

Madeline_4
(portal protein)

Madeline_5
(capsid maturation protease)

Madeline_6
(major capsid protein)

Madeline_7

Madeline_8
(head-to-tail adaptor)

Madeline_9
(head-to-tail stopper)

Madeline_10

Madeline_11
(tail terminator)

Madeline_12
(major tail protein)

Madeline_13

Madeline_14
(tail assembly chaperone)

Madeline_15
(tail assembly chaperone)

Madeline_16
(membrane protein)

Madeline_17
(tape measure protein)

Madeline_18
(minor tail protein)

Madeline_19
(minor tail protein)

Madeline_20
(lysin A, L-Ala-D-Glu peptidase domain)

Madeline_21
(lysin A, glycosyl hydrolase domain)

Madeline_22
(membrane protein)

Madeline_23
(membrane protein)

Madeline_24
(membrane protein)

Madeline_25
(lysin B)

Madeline_26
(minor tail protein)

Madeline_27

Madeline_28
(minor tail protein)

Madeline_29

Madeline_30

Madeline_31
(membrane protein)

Madeline_32
(helix-turn-helix DNA binding domain protein)

Madeline_33
(BrnT-like toxin)

Madeline_35
(tyrosine integrase)

Madeline_36
(helix-turn-helix DNA binding domain protein)

Madeline_37

Madeline_38
(metalloprotease)

Madeline_39

Madeline_40

Madeline_41
(immunity repressor)

Madeline_42
(helix-turn-helix DNA binding domain protein)

Madeline_43

Madeline_44
(antirepressor)

Madeline_45

Madeline_46

Madeline_47
(helix-turn-helix DNA binding domain protein)

Madeline_48
(membrane protein)

Madeline_49

Madeline_50

Madeline_51

Madeline_52

Madeline_53

Madeline_54

Madeline_55
(DNA polymerase III subunit)

Madeline_56

Madeline_57
(RecE-like exonuclease)

Madeline_58
(RecT-like ssDNA binding protein)

Madeline_59

Madeline_60

Madeline_61
(DNA methylase)

Madeline_62

Madeline_63

Madeline_64

Madeline_65
(helix-turn-helix DNA binding domain protein)

Madeline_66

Madeline_67

Madeline_68
(RusA-like resolvase)

Madeline_69

Madeline_70

Madeline_71

Madeline_72
(helix-turn-helix DNA binding domain protein)

Madeline_73

Madeline_74

Madeline_75

Madeline_76
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK919469
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.33 x 10^11 pfu/mL
Pitt Freezer Box# 90
Pitt Freezer Box Grid# E9
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload