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Mycobacterium phage MikesPholly
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Detailed Information for Phage MikesPholly
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMichael Savoie
Year Found2017
Location FoundCharlton, MA USA
Finding InstitutionWorcester Polytechnic Institute
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates42.1 N, 71.9 E Map
Discovery NotesThe site of soil collection was at the edge of a flower bed that had lot of plant growth and previous soil amenities added. A garden trowel was used to remove the soil and it was deposited in a ziplock lock freezer bag and store at room temperature. Enrichment method was used to isolate the phage from the collected sample. The phage was purified and a phage lysate was collected.
Naming NotesThe name was inspired by my inability to capture a phage in my original sample.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesPlaques were very circular. They were opaque in appearance and very cloudy.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2X10^10
Pitt Freezer Box# 66
Pitt Freezer Box Grid# F2
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload