Mycobacterium phage Moonpi
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| Detailed Information for Phage Moonpi | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Ella Sweeney |
| Year Found | 2020 |
| Location Found | Bowling Green, KY United States |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 36.985412 N, 86.449561 W Map |
| Discovery Notes | The soil sample was collected behind a greenhouse on the Western Kentucky University campus. The soil was damp, had soil and root fragments mixed within it, and had a layer of moss above it. The soil was enriched and purified through a series of serial dilutions that worked to isolate the phage. Once the phage was isolated and of one genotype, a plate was made to collect the lysate from. The lysate was used for an electron microscopy image, the nanodrop calculations, and also the titer calculations. |
| Naming Notes | I named the phage Moonpi because of a saying I have with my Dad, that refers to the trademarked name "MoonPie". |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The plaques produced by the phage Moonpi were lytic with turbid halos surrounding them. The average plaque size, of the isolated Moonpi phage, was 5mm. The phage producing the lytic halo phages was isolated during the purification step in which a plaque was chosen from an enrichment sample plate and diluted until isolated. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.69*10^10 |
| Pitt Freezer Box# | 120 |
| Pitt Freezer Box Grid# | E12 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
