Mycobacterium phage Muphrid
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| Detailed Information for Phage Muphrid | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Natasha Otieno |
| Year Found | 2021 |
| Location Found | Bowling Green, KY United States of America |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 36.986983 N, 86.4488 W Map |
| Discovery Notes | The harvest site was right next to a manhole. It contained very dry soil at surface level and as I dug deeper, it gained moisture. The temperature at this location was 32 degrees Celcius. Small ants and insects were observed moving in and out of the soil. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The plaques in the sample appeared turbid and misshapen with some of the plaques being cut off by other plaques. This is a clear sign of phage mutants present in the sample. The plate does not look overpopulated, containing about 43 countable plaques. Some plaques are clear but most of them are turbid, measuring about 0.4 cm. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 4.3 * 10^8 pfu / mL |
| Pitt Freezer Box# | 137 |
| Pitt Freezer Box Grid# | H8 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
