Mycobacterium phage Nebkiss
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Detailed Information for Phage Nebkiss
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByDeborah Jacobs-Sera
Year Found2015
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramPhage Hunters Integrating Research and Education
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates40.446516 N, 79.954367 W Map
Discovery NotesFound on February 10, 2015 during a phagehunting walk with Billy Biederman (HHMI). this phage was found in a dormant flowerbed in the exact spot where phage Nova was discovered in 2006. the soil was cold, but not frozen solid, outside temperature was around 30C.
Naming NotesNamed after my son who I adore!
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 5, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1116
Genome length (bp)85613
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGCCAGCTG
GC Content56.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterX
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members

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Annotation StatusIn GenBank
Plaque Notes1 mm clear plaques with an accentuated edge.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Nebkiss_1
(terminase small subunit)

Nebkiss_2
(terminase large subunit)

Nebkiss_3
(portal protein)

Nebkiss_4
(scaffolding protein)

Nebkiss_5
(major capsid protein)

Nebkiss_6
(head-to-tail adaptor)

Nebkiss_7
(head-to-tail stopper)

Nebkiss_8

Nebkiss_9
(tail terminator)

Nebkiss_10
(major tail protein)

Nebkiss_11
(tail assembly chaperone)

Nebkiss_12
(tail assembly chaperone)

Nebkiss_13
(tape measure protein)

Nebkiss_14
(minor tail protein)

Nebkiss_15

Nebkiss_16
(DNA binding protein)

Nebkiss_17
(minor tail protein)

Nebkiss_18
(HNH endonuclease)

Nebkiss_19
(HNH endonuclease)

Nebkiss_20

Nebkiss_21
(minor tail protein)

Nebkiss_22
(minor tail protein)

Nebkiss_23
(minor tail protein)

Nebkiss_24
(minor tail protein)

Nebkiss_25

Nebkiss_26

Nebkiss_27
(minor tail protein)

Nebkiss_28
(minor tail protein)

Nebkiss_29
(helix-turn-helix DNA binding protein)

Nebkiss_30

Nebkiss_31

Nebkiss_32
(helix-turn-helix DNA binding protein)

Nebkiss_33

Nebkiss_34
(lysin A)

Nebkiss_35
(lysin B)

Nebkiss_36
(membrane protein)

Nebkiss_37

Nebkiss_38

Nebkiss_39
(helix-turn-helix DNA binding protein)

Nebkiss_40

Nebkiss_41

Nebkiss_42

Nebkiss_43
(Lsr2-like DNA bridging protein)

Nebkiss_44

Nebkiss_45

Nebkiss_46

Nebkiss_47

Nebkiss_48

Nebkiss_49

Nebkiss_50

Nebkiss_51

Nebkiss_52

Nebkiss_53

Nebkiss_54

Nebkiss_55
(excisionase)

Nebkiss_56
(integrase)

Nebkiss_58

Nebkiss_59

Nebkiss_60

Nebkiss_61

Nebkiss_62

Nebkiss_63

Nebkiss_64

Nebkiss_65

Nebkiss_66
(helix-turn-helix DNA binding protein)

Nebkiss_67

Nebkiss_68
(immunity repressor)

Nebkiss_69
(helix-turn-helix DNA binding protein)

Nebkiss_70
(esterase)

Nebkiss_71

Nebkiss_72
(WhiB family transcription factor)

Nebkiss_73

Nebkiss_74

Nebkiss_75

Nebkiss_76

Nebkiss_77

Nebkiss_78

Nebkiss_79

Nebkiss_80

Nebkiss_81

Nebkiss_82

Nebkiss_83

Nebkiss_84
(HNH endonuclease)

Nebkiss_85
(RNA polymerase II subunit)

Nebkiss_86

Nebkiss_87
(endonuclease VII)

Nebkiss_88
(ribonucleotide reductase)

Nebkiss_89
(thymidylate synthase)

Nebkiss_90

Nebkiss_91

Nebkiss_92

Nebkiss_93

Nebkiss_94
(DNA primase/polymerase)

Nebkiss_95

Nebkiss_96

Nebkiss_97

Nebkiss_98

Nebkiss_99

Nebkiss_100

Nebkiss_101

Nebkiss_102
(DNA helicase)

Nebkiss_103
(PAPS reductase-like domain protein)

Nebkiss_104
(ParB-like nuclease domain protein)

Nebkiss_105
(GIY-YIG-like endonuclease)

Nebkiss_106

Nebkiss_107

Nebkiss_108

Nebkiss_109

Nebkiss_110
(RepA-like helicase)

Nebkiss_111

Nebkiss_112

Nebkiss_113

Nebkiss_114

Nebkiss_115

Nebkiss_116

Nebkiss_117

Nebkiss_118

Nebkiss_119
(]protease)

Nebkiss_120
(DnaQ-like exonuclease)

Nebkiss_121

Nebkiss_122

Nebkiss_123
(HNH endonuclease)

Nebkiss_124

Nebkiss_125

Nebkiss_126
(deoxycytidylate deaminase)

Nebkiss_127
(MazG-like nucleotide pyrophosphohydrolase)

Nebkiss_128

Nebkiss_129

Nebkiss_130
(DNA polymerase III sliding clamp (beta))

Nebkiss_131

Nebkiss_132
(DNA binding domain protein)

Nebkiss_133

Nebkiss_134

Nebkiss_135

Nebkiss_136

Nebkiss_137

Nebkiss_138

Nebkiss_139

Nebkiss_140

Nebkiss_141

Nebkiss_142

Nebkiss_145

Nebkiss_146
(helix-turn-helix DNA binding domain protein)

Nebkiss_147

Nebkiss_148

Nebkiss_149

Nebkiss_150

Nebkiss_151
(dsDNA break-binding protein)

Nebkiss_152

Nebkiss_153

Nebkiss_154

Nebkiss_155

Nebkiss_156

Nebkiss_157

Nebkiss_158

Nebkiss_159

Nebkiss_160

Nebkiss_161

Nebkiss_162

Nebkiss_163

Nebkiss_164

Nebkiss_165
(HNH endonuclease)

Nebkiss_166

Nebkiss_167
(methyltransferase)

Nebkiss_168
(hydrolase domain protein)

Nebkiss_169

Nebkiss_170

Nebkiss_171

Nebkiss_172

Nebkiss_173

Nebkiss_174

Nebkiss_175

Nebkiss_176

Nebkiss_177

Nebkiss_178

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK016501
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 7
Pitt Freezer Box Grid# A3
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload