Gordonia phage Neobush
Know something about this phage that we don't? Modify its data.
Detailed Information for Phage Neobush
Discovery Information
Isolation HostGordonia terrae 3612
Found ByAakriti Neopaney (Adopted by Madeline Bush)
Year Found2019
Location FoundPittsburgh, PA United States
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates40.340556 N, 79.970278 W Map
Discovery NotesMy soil sample was collected from the flowerbed in the backyard of my house. My house is located in Baldwin, PA. I collected my soil sample on Monday, September 23rd 2019 at 7:48AM. The soil was slightly moist and covered in dead roots. The soil was collected directly from the surface. It was gloomy outside with an ambient temperature of 67º F. There were a few plants behind the area in which I collected the soil from.
Naming NotesPhage Neobush was isolated and discovered by Neopaney and adopted by Bush. The parents of the phage decided to combine their last names for their special child that was found on the sixth isolation trial.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 24, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1775
Genome length (bp)50906
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGCTGGGGA
GC Content66.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCZ
SubclusterCZ1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionWashington State University
Annotation StatusIn GenBank
Has been Phamerated?Yes
Gene List

Click to View

Neobush_1
(terminase, small subunit)

Neobush_2
(terminase, large subunit)

Neobush_3

Neobush_4
(portal protein)

Neobush_5
(capsid maturation protease)

Neobush_6
(major capsid protein)

Neobush_7

Neobush_8
(head-to-tail adaptor)

Neobush_9
(head-to-tail stopper)

Neobush_10

Neobush_11
(tail terminator)

Neobush_12
(major tail protein)

Neobush_13

Neobush_14
(tail assembly chaperone)

Neobush_15
(tail assembly chaperone)

Neobush_16

Neobush_17
(tape measure protein)

Neobush_18
(minor tail protein)

Neobush_19
(minor tail protein)

Neobush_20
(lysin A, L-Ala-D-Glu peptidase domain)

Neobush_21

Neobush_22
(Lysin A, glycosyl hydrolase domain)

Neobush_23

Neobush_24

Neobush_25

Neobush_26

Neobush_27
(lysin B)

Neobush_28
(minor tail protein)

Neobush_29

Neobush_30
(minor tail protein)

Neobush_31

Neobush_32
(metalloprotease)

Neobush_33

Neobush_34

Neobush_35
(helix-turn-helix DNA binding domain)

Neobush_36

Neobush_37

Neobush_38

Neobush_39
(tyrosine integrase)

Neobush_40

Neobush_41

Neobush_42

Neobush_43
(helix-turn-helix DNA binding domain)

Neobush_44
(immunity repressor)

Neobush_45
(helix-turn-helix DNA binding domain)

Neobush_46

Neobush_47

Neobush_48
(antirepressor)

Neobush_49

Neobush_50
(excise)

Neobush_51

Neobush_52

Neobush_53

Neobush_54

Neobush_55

Neobush_56
(RecE-like exonuclease)

Neobush_57
(RecT-like ss DNA binding protein)

Neobush_58

Neobush_59

Neobush_60

Neobush_61
(ASC-1 transciption coactivator)

Neobush_62

Neobush_63

Neobush_64
(HNH endonuclease)

Neobush_65
(DNA methylase)

Neobush_66

Neobush_67

Neobush_68

Neobush_69

Neobush_70

Neobush_71

Neobush_72

Neobush_73
(RusA-like resolvase (endonuclease))

Neobush_74

Neobush_75

Neobush_76

Neobush_77
(helix-turn-helix DNA binding domain)

Neobush_78

Neobush_79

Neobush_80

Neobush_81
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMW924650
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 114
Pitt Freezer Box Grid# H2
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload