Mycobacterium phage Oaker
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Detailed Information for Phage Oaker
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByDanielle DeNigris
Year Found2012
Location FoundPhiladelphia, PA United States
Finding InstitutionSaint Joseph's University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates39.971361 N, 75.194733 W Map
Discovery NotesSoil taken from gazelle exhibit at the Philadelphia Zoo. Soil was in a shady area near kopje and was 2-2.5 cm deep.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 19, 2013
Sequencing FacilityVirginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage250
Genome length (bp)69099
Character of genome endsCircularly Permuted
GC Content57.5%
Fasta file available?Yes: Download fasta file
Cluster Life CycleLytic
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesThe plaques were fast growing appearing after about 28 hours but not as soon as 24 hours. The plaques were small (about 1 mm in diameter) and very clear.
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKF986247
Refseq NumberNC_023578
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Designator 2012STJOoakerDMD
SEA Lysate Titer 6.1 x 10^10 pfu/mL
Date of SEA Lysate Titering Oct 18, 2012
Pitt Freezer Box# 39
Pitt Freezer Box Grid# 37
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload