Mycobacterium phage OmegaMRC
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|Detailed Information for Phage OmegaMRC|
|Isolation Host||Mycobacterium smegmatis mc²155|
|Found By||Team Plaque It Up|
|Location Found||Santa Monica , CA USA|
|Finding Institution||University of California, Los Angeles|
|Program||Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science|
|From enriched soil sample?||No|
|GPS Coordinates||34.093182 N, 118.587315 W Map|
|Discovery Notes||Phage OmegaMRC was isolated from soil, collected on 10/08/17 by O. Monifa Sawyerr at a depth of about 3 inches. This sample was a damp soil sample collected around a shrubby area in Santa Monica. There was a lot of plastic and cigarette butts in the area. There was also a lot of tree debris such as leaves and bark covering the area. Air temp at time of collection was 26.7˚C. |
OmegaMRC was isolated by direct plating at 35C.
Characterization was performed by Team Plaque It Up (Carly Pierson, O. Monifa Sawyerr, Rina Watanabe).
Midi-Lysate (Archive) Titer: 4.93*10^14 PFU/mL
Date of High-titer Lysate Titering: 11/1/17
MIMG103AL-F17 Instructor: Dr. Amanda Freise
Teaching Assistant: Andrew Lund
Undergraduate Assistant: Keith Mitchell
Laboratory Support: Dr. Krisanavane Reddi and Dr. Jordan Moberg Parker
|Naming Notes||We named our phage OmegaMRC because Omega means great and we wanted a connection of our phage to us, so we included our team's first initials.|
|Genome length (bp)||Unknown|
|Character of genome ends||Unknown|
|Fasta file available?||No|
|Annotating Institution||Unknown or unassigned|
|Annotation Status||Not sequenced|
|Plaque Notes||The plaque morphology varies between halo and bullseye. The initial plaque picked was a bullseye plaque. From there, it has taken off and grown into a halo plaque |
Average size (and size range) diameter in mm: The average size is approx. 6mm.
|Has been Phamerated?||No|
|Uploaded to GenBank?||No|
|GenBank Accession||None yet|
|Refseq Number||None yet|
|Pitt Freezer Box#||58|
|Pitt Freezer Box Grid#||F5|