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Mycobacterium phage OmegaMRC
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Detailed Information for Phage OmegaMRC
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByTeam Plaque It Up
Year Found2017
Location FoundSanta Monica , CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature35°C
GPS Coordinates34.093182 N, 118.587315 W Map
Discovery NotesPhage OmegaMRC was isolated from soil, collected on 10/08/17 by O. Monifa Sawyerr at a depth of about 3 inches. This sample was a damp soil sample collected around a shrubby area in Santa Monica. There was a lot of plastic and cigarette butts in the area. There was also a lot of tree debris such as leaves and bark covering the area. Air temp at time of collection was 26.7˚C.

OmegaMRC was isolated by direct plating at 35C.

Characterization was performed by Team Plaque It Up (Carly Pierson, O. Monifa Sawyerr, Rina Watanabe).

Midi-Lysate (Archive) Titer: 4.93*10^14 PFU/mL
Date of High-titer Lysate Titering: 11/1/17

MIMG103AL-F17 Instructor: Dr. Amanda Freise
Teaching Assistant: Andrew Lund
Undergraduate Assistant: Keith Mitchell
Laboratory Support: Dr. Krisanavane Reddi and Dr. Jordan Moberg Parker
Naming NotesWe named our phage OmegaMRC because Omega means great and we wanted a connection of our phage to us, so we included our team's first initials.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesThe plaque morphology varies between halo and bullseye. The initial plaque picked was a bullseye plaque. From there, it has taken off and grown into a halo plaque
Average size (and size range) diameter in mm: The average size is approx. 6mm.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 58
Pitt Freezer Box Grid# F5
Available Files
Plaque PictureDownload
EM PictureDownload