Mycobacterium phage PRicky
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| Detailed Information for Phage PRicky | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Valeria Villanueva |
| Year Found | 2017 |
| Location Found | Baltimore, MD USA |
| Finding Institution | Johns Hopkins University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 39.3311 N, 76.6196 E Map |
| Discovery Notes | - Performed enriched isolation and plated the sample on a spot test and discovered plaques. - Performed 4 rounds of serial dilution and obtained a pure population of phage with consistent results throughout the various rounds. - Made a webbed plate with the last round of serial dilution and flooded it to obtain my lysate. - Titer was too low at first, but with more webbed plates, I got a titer high enough for DNA prep. |
| Naming Notes | Named my phage PRicky, inspired by the show Rick and Morty. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | From the beginning, my phage's plaque morphology was medium-large (about 0.3-0.5 cm in diameter), round, and clear in the center with a few plaques having cloudy edges. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 71 |
| Pitt Freezer Box Grid# | A2 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |