| Detailed Information for Phage Pakusa |
| Discovery Information |
| Isolation Host | Gordonia rubripertincta NRRL B-16540 |
| Found By | Kashmala Mahmood |
| Year Found | 2022 |
| Location Found | Sterling, VA United States |
| Finding Institution | George Mason University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 39.009392 N, 77.378712 W Map |
| Discovery Notes | The sample was collected from the side of a relatively clear lake on the morning of August 29th. The location was a few feet off from a rough path, which divided the water body from a steep hill. The soil was moist due to contact with the lake water (similar to damp silt), and the sample was taken within 2 inches of the surface. |
| Naming Notes | The name of the phage is a reference to the discoverer's mother and birth nations. It is a concatenation of the former's first three letters and the acronym of the latter. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 23, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1214 |
| Genome length (bp) | 88438 |
| Character of genome ends | Circularly Permuted |
| GC Content | 60.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | DQ |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | George Mason University |
| Annotation Status | Being Annotated (Expected completion by 5/1/2023) |
| Plaque Notes | Small (about 1 mm), turbid plaques. No halos were seen and it was assumed that the plaques were temperate. |
| Morphotype | Myoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
161 |
| Pitt Freezer Box Grid# |
E4 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
