Mycobacterium phage Parmesanjohn
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Detailed Information for Phage Parmesanjohn
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByRyan Seth and Sarah Misiukiewicz
Year Found2017
Location FoundBethlehem, PA United States of America
Finding InstitutionLehigh University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.604448 N, 75.374991 W Map
Discovery NotesFound outside of the Dravo building at Lehigh University
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 25, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage201
Genome length (bp)43700
Character of genome ends3' Sticky Overhang
Overhang Length13 bases
Overhang SequenceCCCGCCGCAATGG
GC Content66.3%
Sequencing Notesphage was sequenced as part of a DOGEMS sample from Lehigh 2017

at position 18963 bp approximately 30% of reads contain an A instead of consensus T
Fasta file available?Yes: Download fasta file
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusOverdue (Expected completion by 12/15/2018)
Plaque NotesPlaques have diameter of 2 mm and appear 24-48 hours after plating. Center of each plaque is clear and becomes more turbid as you travel further from the center.
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.67E10 pfu/ml
Date of SEA Lysate Titering Mar 19, 2017
Pitt Freezer Box# 51
Pitt Freezer Box Grid# C11
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload