Mycobacterium phage Parmesanjohn
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Detailed Information for Phage Parmesanjohn
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByRyan Seth and Sarah Misiukiewicz
Year Found2017
Location FoundBethlehem, PA USA
Finding InstitutionLehigh University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.604448 N, 75.374991 W Map
Discovery NotesFound outside of the Dravo building at Lehigh University
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 25, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage201
Genome length (bp)43700
Character of genome ends3' Sticky Overhang
Overhang Length13 bases
Overhang SequenceCCCGCCGCAATGG
GC Content66.3%
Sequencing Notesphage was sequenced as part of a DOGEMS sample from Lehigh 2017

at position 18963 bp approximately 30% of reads contain an A instead of consensus T
Fasta file available?Yes: Download fasta file
Characterization
ClusterN
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionLehigh University
Annotation StatusIn GenBank
Plaque NotesPlaques have diameter of 2 mm and appear 24-48 hours after plating. Center of each plaque is clear and becomes more turbid as you travel further from the center.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Parmesanjohn_1

Parmesanjohn_2
(terminase)

Parmesanjohn_3

Parmesanjohn_4
(portal protein)

Parmesanjohn_5
(capsid maturation protease)

Parmesanjohn_6
(major capsid protein)

Parmesanjohn_7

Parmesanjohn_8
(head-to-tail connector complex protein)

Parmesanjohn_9
(head-to-tail stopper)

Parmesanjohn_10

Parmesanjohn_11

Parmesanjohn_12
(tail terminator)

Parmesanjohn_13
(major tail protein)

Parmesanjohn_14
(tail assembly chaperone)

Parmesanjohn_15
(tail assembly chaperone)

Parmesanjohn_16
(tape measure protein)

Parmesanjohn_17
(minor tail protein)

Parmesanjohn_18
(minor tail protein)

Parmesanjohn_19
(minor tail protein)

Parmesanjohn_20
(minor tail protein)

Parmesanjohn_21

Parmesanjohn_22

Parmesanjohn_23

Parmesanjohn_24

Parmesanjohn_25

Parmesanjohn_26

Parmesanjohn_27

Parmesanjohn_28
(lysin A)

Parmesanjohn_29
(holin)

Parmesanjohn_30

Parmesanjohn_31

Parmesanjohn_32

Parmesanjohn_33
(HicB-like antitoxin)

Parmesanjohn_34

Parmesanjohn_35
(membrane protein)

Parmesanjohn_36
(integrase)

Parmesanjohn_37
(immunity repressor)

Parmesanjohn_38
(excise)

Parmesanjohn_39

Parmesanjohn_40

Parmesanjohn_41

Parmesanjohn_42

Parmesanjohn_43

Parmesanjohn_44

Parmesanjohn_45
(WhiB family transcription factor)

Parmesanjohn_46

Parmesanjohn_47
(RecE-like exonuclease)

Parmesanjohn_48
(RecT-like ssDNA binding protein)

Parmesanjohn_49
(resolvase)

Parmesanjohn_50
(NrdH-like glutaredoxin)

Parmesanjohn_51

Parmesanjohn_52

Parmesanjohn_53

Parmesanjohn_54

Parmesanjohn_55

Parmesanjohn_56

Parmesanjohn_57

Parmesanjohn_58

Parmesanjohn_59

Parmesanjohn_60

Parmesanjohn_61
(HNH endonuclease)

Parmesanjohn_62

Parmesanjohn_63

Parmesanjohn_64
(helix-turn-helix DNA binding domain protein)

Parmesanjohn_65
(DnaC-like helicase loader)

Parmesanjohn_66

Parmesanjohn_67

Parmesanjohn_68

Parmesanjohn_69

Parmesanjohn_70

Parmesanjohn_71

Parmesanjohn_72
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK524515
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.67E10 pfu/ml
Date of SEA Lysate Titering Mar 19, 2017
Pitt Freezer Box# 51
Pitt Freezer Box Grid# C11
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload