Microbacterium phage PhillyPhilly
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Detailed Information for Phage PhillyPhilly
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224 SEA
Found ByJack Kisielius
Year Found2018
Location FoundPhiladelphia, PA USA
Finding InstitutionUniversity of the Sciences in Philadelphia
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature24°C
GPS Coordinates39.945098 N, 75.207592 W Map
Discovery NotesLittle to no foot traffic from where the sample was collected. Exposed to sunlight; on top of a ridge; and near train tracks.
Naming NotesWe named our phage PhillyPhilly in order to forever commemorate the Philadelphia Eagles winning Super Bowl 52.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 8, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1492
Genome length (bp)62869
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length3380 bp
GC Content62.7%
Sequencing NotesThis phage was sequenced as part of a mixed DOGEMS sample and later identified by PCR.
Fasta file available?Yes: Download fasta file
Characterization
ClusterED
SubclusterED1
Cluster Life CycleLytic
Other Cluster Members
Annotation StatusBeing Annotated (Expected completion by 5/15/2019)
Plaque NotesMultiple plaque sizes, one size was 1 mm and the other plaque size was around 2.5 mm. Clear plaques were formed.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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PhillyPhilly_1

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PhillyPhilly_119

Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 4.8 x 10^9 pfu/mL
Pitt Freezer Box# 84
Pitt Freezer Box Grid# E3
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload