Mycobacterium phage Phlash4
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| Detailed Information for Phage Phlash4 | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | J. Valdez, N. Ayvar, J. Lipp, P. McKinzie |
| Year Found | 2021 |
| Location Found | Longview, TX United States |
| Finding Institution | LeTourneau University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 32.464611 N, 94.727161 W Map |
| Discovery Notes | The soil sample was collected in a small garden under a bush that provided plenty of shade. The weather was 29.4 degrees Celsius, mostly sunny, and a little humid. We collected the sample at 10:20 in the morning and dug about 1 inch deep before collecting soil. The soil we collected was dark, thick, and had some worms crawling around. |
| Naming Notes | Our group came up with the name Phalsh4 because we ran through the processes of purification quickly and accurately. We jumped ahead of most of the class giving us the Phlash name. The 4 in the name is referring to the four guys that participated in the experiment. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | We had two sizes of plaques throughout purification. We had some micro plaques due to a heat shock in the incubator which we could not get rid of during purification. The normal size plaques had an average diameter of 0.45cm. The micro plaques had an average diameter of 0.1cm. The plaques had clear centers, and rigid outsides after 48 hours of incubation. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 134 |
| Pitt Freezer Box Grid# | D7 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |