Mycobacterium phage Phlash4
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Detailed Information for Phage Phlash4
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJ. Valdez, N. Ayvar, J. Lipp, P. McKinzie
Year Found2021
Location FoundLongview, TX United States
Finding InstitutionLeTourneau University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates32.464611 N, 94.727161 W Map
Discovery NotesThe soil sample was collected in a small garden under a bush that provided plenty of shade. The weather was 29.4 degrees Celsius, mostly sunny, and a little humid. We collected the sample at 10:20 in the morning and dug about 1 inch deep before collecting soil. The soil we collected was dark, thick, and had some worms crawling around.
Naming NotesOur group came up with the name Phalsh4 because we ran through the processes of purification quickly and accurately. We jumped ahead of most of the class giving us the Phlash name. The 4 in the name is referring to the four guys that participated in the experiment.
Sequencing Information
Sequencing Complete?No
Sequencing FacilityPittsburgh Bacteriophage Institute
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesWe had two sizes of plaques throughout purification. We had some micro plaques due to a heat shock in the incubator which we could not get rid of during purification. The normal size plaques had an average diameter of 0.45cm. The micro plaques had an average diameter of 0.1cm. The plaques had clear centers, and rigid outsides after 48 hours of incubation.
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload